Transplantation of Human Neural Progenitor Cells into the Neonatal Rat Brain: Extensive Migration and Differentiation with Long-Distance Axonal Projections

Englund, Ulrica; Fricker-Gates, Rosemary A.; Lundberg, Cecilia; Björklund, Anders; Wictorin, Klas

doi:10.1006/exnr.2001.7750

Cited by 174 publications

(123 citation statements)

References 65 publications

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“…We hypothesize that the intact or lesioned brain environment does not provide those signals which results in a block on division and differentiation into neurons. Clearly, transplantation of similar cells into a neurogenic environment, such as the neonatal rat brain, sub ventricular zone of the forebrain or the hippocampus, does lead to neuron formation (7,8), perhaps through exposure to the correct proliferation signals and a final round of division within the brain.…”

Section: Discussionmentioning

confidence: 99%

Lesion-Induced Increase in Survival and Migration of Human Neural Progenitor Cells Releasing GDNF

et al. 2008

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The use of human neural progenitor cells (hNPC) has been proposed to provide neuronal replacement or astrocytes delivering growth factors for brain disorders such as Parkinson's and Huntington's disease. Success in such studies likely requires migration from the site of transplantation and integration into host tissue in the face of ongoing damage. In the current study, hNPC modified to release glial cell line derived neurotrophic factor (hNPC GDNF ) were transplanted into either intact or lesioned animals. GDNF release itself had no effect on the survival, migration or differentiation of the cells. The most robust migration and survival was found using a direct lesion of striatum (Huntington's model) with indirect lesions of the dopamine system (Parkinson's model) or intact animals showing successively less migration and survival. No lesion affected differentiation patterns. We conclude that the type of brain injury dictates migration and integration of hNPC which has important consequences when considering transplantation of these cells as a therapy for neurodegenerative diseases.

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Section: Discussionmentioning

confidence: 99%

Lesion-Induced Increase in Survival and Migration of Human Neural Progenitor Cells Releasing GDNF

et al. 2008

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“…Following lethal exposure to CO 2 the embryos were removed and either taken for FACS analysis or immersion fixed overnight in 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS; 0.1 M) at 8°C. Early stage (E10.5 -E13.5) or late stage (E15.5-17.5) fixed embryos were cryoprotected in 30% or 25% sucrose respectively and cryo-sectioned at a thickness of [12][13][14][15][16] µm.…”

Section: Animals and Tissue Preparationmentioning

confidence: 99%

“…The procedure for immunostaining cell cultures or tissue sections was carried out as previously described (12). Briefly, sections and culture slides were preincubated for 1 hour in blocking solution containing 5% normal serum and 0.25% triton-X (Ameresco) in KPBS.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Neurogenin2 identifies a transplantable dopamine neuron precursor in the developing ventral mesencephalon

Thompson

Andersson

Jensen

et al. 2006

Experimental Neurology

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“…Or is that capacity exclusive of the SVZ cells? While the SVZ/RMS could sustain migration of some exogenous progenitor cell populations (61)(62)(63), cells from the embryonic ventricular zone and external granular layer failed to migrate when transplanted into the postnatal SVZ (57,64). In spite of their normal radial migration it could be expected that external granular layer and ventricular zone cells would respond to the local cues of the postnatal SVZ/RMS, for, as we have seen above, many factors involved in radial migration are also present in RMS migrating cells.…”

Section: Long and Winding Road: Instruction Or Restriction?mentioning

confidence: 99%

Cell migration in the postnatal subventricular zone

Menezes

Marins

Alves

et al. 2002

Braz J Med Biol Res

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New neurons are constantly added to the olfactory bulb of rodents from birth to adulthood. This accretion is not only dependent on sustained neurogenesis, but also on the migration of neuroblasts and immature neurons from the cortical and striatal subventricular zone (SVZ) to the olfactory bulb. Migration along this long tangential pathway, known as the rostral migratory stream (RMS), is in many ways opposite to the classical radial migration of immature neurons: it is faster, spans a longer distance, does not require radial glial guidance, and is not limited to postmitotic neurons. In recent years many molecules have been found to be expressed specifically in this pathway and to directly affect this migration. Soluble factors with inhibitory, attractive and inductive roles in migration have been described, as well as molecules mediating cell-to-cell and cell-substrate interactions. However, it is still unclear how the various molecules and cells interact to account for the special migratory behavior in the RMS. Here we will propose some candidate mechanisms for roles in initiating and stopping SVZ/RMS migration.

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Transplantation of Human Neural Progenitor Cells into the Neonatal Rat Brain: Extensive Migration and Differentiation with Long-Distance Axonal Projections

Cited by 174 publications

References 65 publications

Lesion-Induced Increase in Survival and Migration of Human Neural Progenitor Cells Releasing GDNF

Lesion-Induced Increase in Survival and Migration of Human Neural Progenitor Cells Releasing GDNF

Neurogenin2 identifies a transplantable dopamine neuron precursor in the developing ventral mesencephalon

Cell migration in the postnatal subventricular zone

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