Transplantation of in vitro—cultured epidermis bearing melanocytes for repigmenting vitiligo

Falabella, Rafael; Escobar, Carlos; Borrero, Isabella

doi:10.1016/s0190-9622(89)70170-5

Cited by 84 publications

(37 citation statements)

References 34 publications

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“…They are as follows: a shave biopsy is taken with the help of a silver's skin grafting knife (E. Murray Co., UK) or a Goulian skin graft knife (E Weck Co., NJ, USA) from the gluteal area or upper thigh. The specimen is transferred to 0.25% trypsin and incubated for a varying period of 15 min to 3 h [58][59][60][61]. After incubation, the skin sample is washed, trypsin is neutralized and epidermis is separated from the dermis mechanically to obtain epidermal cells.…”

Section: Cellular Grafting: Noncultured and Cultured Techniquesmentioning

confidence: 99%

Surgical management of vitiligo

Mulekar¹

2010

Expert Review of Dermatology

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Vitiligo is a common condition characterized by hypopigmented and/or depigmented spots on the skin, affecting approximately 1-2% of the world population. Until approximately 30 years ago, it was exclusively treated by medical therapies. The most commonly used medical therapies include topical steroids, calcineurin inhibitors and phototherapy. Lesions on the face and neck respond the best to medical therapies, followed by lesions on the proximal extremities and trunk, while those distributed over acral parts of extremities and nonhairy areas, such as the wrist, feet and male genitals, respond poorly. Surgical methods complement medical therapies by providing melanocytes to these refractory lesions, and are indicated for unilateral segmental and clinically stable bilateral vitiligo, refractory to medical treatment. Tissue-grafting methods include minigrafting or punch grafting, epidermal grafting and split thickness grafting. These methods use full-thickness punch grafts, roof of epidermal blisters and shave biopsy samples, respectively, as the source of melanocytes. Cellular grafting includes noncultured and cultured melanocytes/keratinocytes suspensions obtained from trypsinizing the shaved skin biopsy sample as a source of melanocytes. Recent advances over the years have enabled dermatologists to treat extensive areas located on any anatomic site in a single operative session by surgical intervention. The purpose of this review is to describe the selection criteria for the surgical treatment, their utility and limitations in the various types of vitiligo.

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Section: Cellular Grafting: Noncultured and Cultured Techniquesmentioning

confidence: 99%

Surgical management of vitiligo

Mulekar¹

2010

Expert Review of Dermatology

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“…[26][27][28] The use of autologous epidermal cultures bearing melanocytes 29,30 has also been proposed for the surgical treatment of stable vitiligo. [31][32][33][34][35][36] This technique, although promising, is still in its embryonic stage, since the number of patients treated is very limited. 18 Moreover, data concerning the amount of melanocytes transplanted, the reproducibility of the technique, and the longterm clinical results are inconclusive.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Treatment of "Stable" Vitiligo by Timedsurgery and Transplantation of Cultured Epidermal Autografts

et al. 2000

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“…48 They are used for resurfacing skin in cases like extensive burns, chronic ulcers, recalcitrant epidermolysis bullosa and stable vitiligo. 49,50,51 NJDVL -13…”

Section: Cultured Skin Graftsmentioning

confidence: 99%

Surgical management of vitiligo - An approach to the patient

Manchanda

Bansal

Pandey

2013

Nepal J Dermatol Venereol & Leprol

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Vitiligo vulgaris is an acquired chronic pigmentary disorder with an average world wide incidence of approximately 1%. Clinical classification of vitiligo is based on the distribution and extension of lesions as localized, generalized, and universal. As vitiligo has great psychosocial implications especially in dark-skinned individuals, treatment should begin early. Currrent modalities of treatment include medical therapy, surgical modalities and laser. Medical treatment options include topical corticosteroids, calcineurin inhibitors, vitamin-D derivatives, phototherapy (ultraviolet A [UVA], narrowband UVB), photochemotherapy (psoralen plus UVA [PUVA]. Surgical methods used in the management of vitiligo gave cosmetically acceptable results in short periods. Successful treatment with various surgical techniques requires an appropriate selection of patients mainly focusing upon the stability of the clinical disease. The choice of the procedure always depends upon the age, the area involved, site and the cosmetic need of the patient. The nature of the procedure, expected outcome and the complications should be discussed with the patient in detail. This article mainly deals with various dermatosurgical approaches in brief available till date for the treatment of vitiligo.

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Transplantation of in vitro—cultured epidermis bearing melanocytes for repigmenting vitiligo

Cited by 84 publications

References 34 publications

Surgical management of vitiligo

Surgical management of vitiligo

Treatment of "Stable" Vitiligo by Timedsurgery and Transplantation of Cultured Epidermal Autografts

Surgical management of vitiligo - An approach to the patient

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