Transplantation of isologous islets of langerhans in diabetic rats

Lorenz, D.; Petermann, J; Beckert, R; Rosenbaum, K D; Ziegler, M.; Dorn, A.

doi:10.1007/bf02581110

Cited by 22 publications

(7 citation statements)

References 6 publications

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“…The peritoneum offers an unlimited space for transplanted islets and therefore offers an attractive site for concurrent use with encapsulated devices to protect the islets [81, 82]; however recovery of these islets for histological and functional assessments is difficult [83]. In rats, at least 1500 islets were required to reverse diabetes, and due to a lack of parasympathetic reinnervation at this site, abnormal glucose tolerance tests were noted [84].…”

Section: Hepatic and Alternative Transplant Sitesmentioning

confidence: 99%

Revascularization of Transplanted Pancreatic Islets and Role of the Transplantation Site

Pepper

Gala-López

Ziff

et al. 2013

Clinical and Developmental Immunology

138

101

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Since the initial reporting of the successful reversal of hyperglycemia through the transplantation of pancreatic islets, significant research efforts have been conducted in elucidating the process of revascularization and the influence of engraftment site on graft function and survival. During the isolation process the intrinsic islet vascular networks are destroyed, leading to impaired revascularization after transplant. As a result, in some cases a significant quantity of the beta cell mass transplanted dies acutely following the infusion into the portal vein, the most clinically used site of engraftment. Subsequently, despite the majority of patients achieving insulin independence after transplant, a proportion of them recommence small, supplemental exogenous insulin over time. Herein, this review considers the process of islet revascularization after transplant, its limiting factors, and potential strategies to improve this critical step. Furthermore, we provide a characterization of alternative transplant sites, analyzing the historical evolution and their role towards advancing transplant outcomes in both the experimental and clinical settings.

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Section: Hepatic and Alternative Transplant Sitesmentioning

confidence: 99%

Revascularization of Transplanted Pancreatic Islets and Role of the Transplantation Site

Pepper

Gala-López

Ziff

et al. 2013

Clinical and Developmental Immunology

138

101

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“…The peritoneum has been investigated for transplantation of free islets, as it does not limit the amount of transplanted tissue. Unfortunately, the intraperitoneal site is inferior to others from a functional and histological standpoint70. A minimum implantation mass of 1500 islets per rat is required to reverse hyperglycaemia, and the lack of parasympathetic reinnervation of islets at this site is associated with abnormal glucose tolerance tests35.…”

Section: Animal Modelsmentioning

confidence: 99%

Optimal implantation site for pancreatic islet transplantation

Merani

Toso

Emamaullee

et al. 2008

British Journal of Surgery

229

173

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“…Unfortunately, studies in the murine model showed that a high number of islets are required for hyperglycemia reversion and the lack of parasympathetic re-innervation of the graft is associated with abnormal glucose tolerance tests [65] and morphologic alteration in islet architecture [66]. The peritoneum has the advantages of accepting both unpurified and microencapsulated islets as tested in many experimental studies.…”

Section: Is It Possible To Overcome Early Challenges Of Intraliver Ismentioning

confidence: 98%

Alternative Transplantation Sites for Pancreatic Islet Grafts

Cantarelli¹,

Piemonti²

2011

Curr Diab Rep

126

100

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The liver is the current site of choice for pancreatic islet transplantation, even though it is far from being an ideal site because of immunologic, anatomic, and physiologic factors leading to a significant early graft loss. A huge amount of alternative sites have been used for islet transplantation in experimental animal models to provide improved engraftment and long-term survival minimizing surgical complications. The pancreas, gastric submucosa, genitourinary tract, muscle, omentum, bone marrow, kidney capsule, peritoneum, anterior eye chamber, testis, and thymus have been explored. Site-specific differences exist in term of islet engraftment, but few alternative sites have potential clinical translation and generally the evidence of a post-transplant islet function better than that reached after intraportal infusion is still lacking. This review discusses site-specific benefits and drawbacks taking into account immunologic, metabolic, and technical aspects to identify the ideal microenvironment for islet function and survival.

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Transplantation of isologous islets of langerhans in diabetic rats

Cited by 22 publications

References 6 publications

Revascularization of Transplanted Pancreatic Islets and Role of the Transplantation Site

Revascularization of Transplanted Pancreatic Islets and Role of the Transplantation Site

Optimal implantation site for pancreatic islet transplantation

Alternative Transplantation Sites for Pancreatic Islet Grafts

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