Transplantation of Male and Female Germ and Somatic Cells

Gosden, Roger G.

doi:10.1007/978-3-662-22189-1_2

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…It is possible that the wide range of developmental stages of follicles in adult mouse ovaries is one of the causes of this tendency. Previous reports describing ovarian histology after freeze-thawing concluded that large mature follicles are more affected by freezing injuries and small immature follicles have a higher survival rate (Parrot 1960, Smith 1961, Gosden 1992, Harp et al 1994, Cox et al 1996, Candy et al 1997. The number of large follicles that survive after freeze-thawing has been estimated to be about 5% of the total surviving follicles (Green et al 1956).…”

Section: Discussionmentioning

confidence: 99%

Fertility of mice receiving vitrified adult mouse ovaries

Hani¹,

Tachibe²,

Shingai³

et al. 2006

Reproduction

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Cryopreservation of the ovaries is a useful technology for preservation of germ cells from experimental animals, because if the female founder is infertile or has mutated mitochondrial DNA, preservation of female germ cells is necessary. Although it is possible to cryopreserve immature mouse ovaries with a high degree of viability by vitrification with a mixture of several cryoprotectants, the viability of cryopreserved adult mouse ovaries is still unknown. Here, we investigated the viability of mouse ovaries at various ages after cryopreservation by vitrification techniques. Donor ovaries were collected from 10-day-, 4-week-, 10-week-and 7-month-old, female, nulliparous, green fluorescence protein (GFP)-transgenic mice and cryopreserved by vitrification. The vitrified-warmed ovaries were orthotopically transplanted to 4-or 10-week-old mice. GFP-positive pups were obtained in all experimental groups. In the 4-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week-and 7-month-old donors were 44%, 9%, 12% and 4% respectively. In the 10-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week-and 7-month-old donors were 36%, 16%, 2% and 9% respectively. Furthermore, GFP-positive pups also were obtained from recipients transplanted with ovaries of donors without normal estrous cyclicity. Our results indicate that cryopreservation of mouse ovaries by vitrification is a useful method for the preservation of female germ cells from mice of various ages.

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Section: Discussionmentioning

confidence: 99%

Fertility of mice receiving vitrified adult mouse ovaries

Hani¹,

Tachibe²,

Shingai³

et al. 2006

Reproduction

View full text Add to dashboard Cite

show abstract

Male germ cell transplantation: an experimental approach with a clinical perspective

Schlatt

Schönfeldt

Schepers

2000

British Medical Bulletin

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Germ cell transplantation was developed in strains of mice. The infusion of germ cell preparations into the seminiferous tubules of infertile hosts led to repopulation of the testis with donor germ cells and restored fertility. Meanwhile, this technique has become a powerful tool to study the expansion of the testicular stem cell population and the kinetics of spermatogonial proliferation and re-induction of spermatogenesis. Further approaches, such as the transfer of rat/hamster/rabbit germ cells into mouse testes or cryopreservation or culture of spermatogonia, have widened the spectrum of applications associated with germ cell transplantation. Since the devastating effects of chemo- or radiotherapy on spermatogonia are the cause of infertility in oncological patients, germ cell transplantation might become an alternative approach for gonadal protection in prepubertal and postpubertal male tumour patients. This review focusses on recent developments with respect to germ cell transplantation and highlights the problems and perspectives of future clinical applications.

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Transplantation of Male and Female Germ and Somatic Cells

Cited by 2 publications

References 38 publications

Fertility of mice receiving vitrified adult mouse ovaries

Fertility of mice receiving vitrified adult mouse ovaries

Male germ cell transplantation: an experimental approach with a clinical perspective

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