Transplantation of motoneurons derived from MASH1-transfected mouse ES cells reconstitutes neural networks and improves motor function in hemiplegic mice

Ikeda, Ritsuko; Kurokawa, Manae S.; Chiba, Shunmei; Yoshikawa, Hideshi; Hashimoto, Tsuyoshi; Tadokoro, Mamoru; Suzuki, Noboru

doi:10.1016/j.expneurol.2004.05.040

Cited by 29 publications

(39 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, to generate neuronal cell types, mammalian achaetescute homolog 1 (mash1) was chosen. Mash1 is a basic helixloop-helix transcription factor that plays a critical role during murine embryonic neurogenesis [38,39] and was shown to be able to drive differentiation of murine ES cells into neuronal lineages [16]. Six days posttransfection of MF-SG with mash1, cells with a neuron-like morphology became apparent (Fig.…”

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Ectopic Expression of Single Transcription Factors Directs Differentiation of a Medaka Spermatogonial Cell Line

Thoma

Wagner

Weber

et al. 2011

Stem Cells and Development

View full text Add to dashboard Cite

The capability to form all cell types of the body is a unique feature of stem cells. However, many questions remain concerning the mechanisms regulating differentiation potential. The derivation of spermatogonial cell lines (SGs) from mouse and human, which can differentiate across germ-layer borders, suggested male germ cells as a potential stem cell source in addition to embryonic stem cells. Here, we present a differentiation system using an SG of the vertebrate model organism Oryzias latipes (medaka). We report differentiation of this cell line into 4 different ectodermal and mesodermal somatic cell types. In addition to differentiation into adipocytes by retinoic acid treatment, we demonstrate for the first time that directed differentiation of an SG can be induced by ectopic expression of single transcription factors, completely independent of culture conditions. Transient transfection with mitf-m, a transcription factor that has been shown to induce differentiation into melanocytes in medaka embryonic stem cells, resulted in the formation of the same cell type in spermatogonia. Similarly, the formation of neuron-like cells and matrix-depositing osteoblasts was induced by ectopic expression of mash1 and cbfa1, respectively. Interestingly, we found that the expression of all mentioned fate-inducing transcription factors leads to recapitulation of the temporal pattern of marker gene expression known from in vivo studies.

show abstract

Section: Resultsmentioning

confidence: 97%

“…But these changes to culture conditions often have to be determined in an empirical and time-consuming fashion. An alternative approach is to enhance lineage-specific differentiation by ectopic expression of transcription factors [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Ectopic Expression of Single Transcription Factors Directs Differentiation of a Medaka Spermatogonial Cell Line

Thoma

Wagner

Weber

et al. 2011

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…Statistically significant differences in the way the walking-beam task is performed by healthy and injured animals up to 20 days after lesion provide insights in to the impact any therapies may have on the dynamics to the recovery of function following brain damage. The rotarod skilled walking task is readily used in various experiments studying functional recovery after brain damage [33][34][35][36][37]. In our model, the use of this in additional testing affords even more complex information on the induced mild sensomotor impairment.…”

Section: Discussionmentioning

confidence: 98%

Structural and functional characteristic of a model for deep-seated lacunar infarct in rats

Janowski

Gornicka-Pawlak

Kozłowska

et al. 2008

Journal of the Neurological Sciences

View full text Add to dashboard Cite

“…[38][39][40][41][42][43][44][45][46][47] For example, several recent studies have highlighted the successful treatment of acute myocardial infarction by injection of autologous bone marrow cells. [48][49][50] Developments in materials science are proving new opportunities for tissue regeneration TE scaffolds have a dual purpose: to direct morphogenesis in vitro and maintain the structure and function of the TE construct as it is integrated with the host tissues postimplantation.…”

Section: Stem Cells Have Great Potential As Therapeutic Agents and Armentioning

confidence: 99%

Gene Therapy Progress and Prospects: In tissue engineering

Polak

Hench

2005

Gene Ther

View full text Add to dashboard Cite

Tissue engineering (TE) has existed for several years as an area spanning many disciplines, including medicine and engineering. The use of stem cells as a biological basis for TE coupled with advances in materials science has opened up an entirely new chapter in medicine and holds the promise of major contributions to the repair, replacement and regeneration of damaged tissues and organs. In this article, we review the spectrum of stem cells and scaffolds being investigated for their potential applications in medicine.

show abstract

Transplantation of motoneurons derived from MASH1-transfected mouse ES cells reconstitutes neural networks and improves motor function in hemiplegic mice

Cited by 29 publications

References 51 publications

Ectopic Expression of Single Transcription Factors Directs Differentiation of a Medaka Spermatogonial Cell Line

Ectopic Expression of Single Transcription Factors Directs Differentiation of a Medaka Spermatogonial Cell Line

Structural and functional characteristic of a model for deep-seated lacunar infarct in rats

Gene Therapy Progress and Prospects: In tissue engineering

Contact Info

Product

Resources

About