Transplantation of porcine umbilical cord matrix cells into the rat brain

Weiss, Mark L.; Mitchell, Kathy E.; Hix, Julie; Medicetty, Satish; El-Zarkouny, S. Z.; Grieger, David M.; Troyer, Deryl L.

doi:10.1016/s0014-4886(03)00128-6

Cited by 106 publications

(85 citation statements)

References 47 publications

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“…MSCs exist in the connective tissue (Wharton's jelly (WJ)) of human umbilical cord and can be harvested easily [214,218,222,223]. Several researchers have demonstrated that umbilical cord-derived stem cells can be differentiated into neuronal phenotype [214,218,222,223] and have demonstrated potential utility in neurodegenerative diseases [224,225]. Our group research has vast experience in the application of mesenchymal stem cells in peripheral nerve regeneration [50,52,53,[56][57][58][59][60][61].…”

Section: Cellular Systemsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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Section: Cellular Systemsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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show abstract

“…There are cells isolated in large numbers from the Wharton's jelly of human cords (25)(26)(27)(28). Two other research labs have published on the isolation and characterization of cells from the Wharton's jelly: Dr. Davies' lab at the University of Toronto (29) and Dr. Y. S. Fu at the National Yang-Ming University, Taipei (30)(31)(32).…”

Section: Characterization Of Umbilical Cord-derived Mscsmentioning

confidence: 99%

“…We have shown that porcine UCM stem cells can be xeno-transplanted into nonimmunesuppressed rats, where they engrafted, proliferated in a controlled fashion, and exhibited TH expression in some cells (27). Most recently, our lab (28), and others (31) have reported that UCM cells ameliorate behavioral deficits in the hemi-parkinsonian rat, and UCM cell transplantation resulted in significantly more dopaminergic neurons in the substantia nigra compared with lesioned, nontransplanted rats that responded to the transplant (28).…”

Section: Properties Of Umbilical Cord Matrix Stem Cellsmentioning

confidence: 99%

“…There is indirect support for an immune-suppressive effect of the MSC-like cells derived from umbilical cord: two labs have transplanted UCM cells xenogenically in nonimmunesuppressed hosts without observation of frank immune rejection (25,27,28,31). In preliminary work, we have found that human UCM cells suppress the proliferation of rat splenocytes exposed to the mitogen ConA, and that a diffusible factor is likely involved (Anderson, Medicetty, and Weiss, unpublished observations).…”

Section: Immune Suppressionmentioning

confidence: 99%

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Stem cells in the umbilical cord

2006

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Stem cells are the next frontier in medicine. Stem cells are thought to have great therapeutic and biotechnological potential. This will not only to replace damaged or dysfunctional cells, but also rescue them and/or deliver therapeutic proteins after they have been engineered to do so. Currently, ethical and scientific issues surround both embryonic and fetal stem cells and hinder their widespread implementation. In contrast, stem cells recovered postnatally from the umbilical cord, including the umbilical cord blood cells, amnion/placenta, umbilical cord vein, or umbilical cord matrix cells, are a readily available and inexpensive source of cells that are capable of forming many different cell types (i.e., they are "multipotent"). This review will focus on the umbilical cord-derived stem cells and compare those cells with adult bone marrow-derived mesenchymal stem cells.

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“…2,3 Thus, they may offer an immediate avenue for cytotherapy, when time is of the essence for such therapy, for example, in cases of malignant neoplasia. UCMS cells transplanted into rodent neurodegenerative models [4][5][6] have been extensively characterized 3 and they express many of the genes expressed by primitive stem cells such as embryonic stem cells (ESCs). 7 They can be differentiated into a variety of cell types including muscle, 8 cartilage, 9 bone, 9 and neural 1 cells and have been used for tissue-engineered artificial blood vessels and heart valves.…”

Section: Introductionmentioning

confidence: 99%

Development of human umbilical cord matrix stem cell-based gene therapy for experimental lung tumors

et al. 2007

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Umbilical cord matrix stem (UCMS) cells are unique stem cells derived from Wharton's jelly, which have been shown to express genes characteristic of primitive stem cells. To test the safety of these cells, human UCMS cells were injected both intravenously and subcutaneously in large numbers into severe combined immunodeficiency (SCID) mice and multiple tissues were examined for evidence of tumor formation. UCMS cells did not form gross or histological teratomas up to 50 days posttransplantation. Next, to evaluate whether UCMS cells could selectively engraft in xenotransplanted tumors, MDA 231 cells were intravenously transplanted into SCID mice, followed by intravenous transplantation of UCMS cells 1 and 2 weeks later. UCMS cells were found near or within lung tumors but not in other tissues. Finally, UCMS cells were engineered to express human interferon beta -designated 'UCMSÀIFN-b'. UCMSÀIFN-b cells were intravenously transplanted at multiple intervals into SCID mice bearing MDA 231 tumors and their effect on tumors was examined. UCMSÀIFN-b cells significantly reduced MDA 231 tumor burden in SCID mouse lungs indicated by wet weight. These results clearly indicate safety and usability of UCMS cells in cancer gene therapy. Thus, UCMS cells can potentially be used for targeted delivery of cancer therapeutics.

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Transplantation of porcine umbilical cord matrix cells into the rat brain

Cited by 106 publications

References 47 publications

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Stem cells in the umbilical cord

Development of human umbilical cord matrix stem cell-based gene therapy for experimental lung tumors

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