Transplantation of primordial germ cells (PGCs) into enucleated oocytes in sheep

This study was performed to culture and preliminarily identify the primordial germ cells (PGCs) isolated from the genital ridge of the Mongolian sheep fetus. The growth characteristics of the sheep PGCs were detected in different culture systems such as culture media, resources, and state and passages of feeder cells. The obtained embryonic germ (EG) cells were identified by morphology, enzymology, and immunofluorescence. The results showed that the sheep EG cell colonies were ridgy, typically nest like, and compact, and had regular edges. Alkaline phosphatase staining reaction was weakly positive. EG cells expressed Kit, Rex-1, Nanog, and Oct-4. Immunofluorescence detection was weakly positive for Oct3/4, whereas positive for SSEA-1, SSEA-3, SSEA-4, TRA-1-61, and TRA-1-80.

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Tissue Maturation in Vivo and in Vitro

Leibfried-Rutledge

Dominko

Critser

et al. 1997

Reproductive Tissue Banking

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Transplantation of primordial germ cells (PGCs) into enucleated oocytes in sheep

Cited by 3 publications

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Chimeric pigs following blastocyst injection of transgenic porcine primordial germ cells

Chimeric pigs following blastocyst injection of transgenic porcine primordial germ cells

Isolation, culture, and characterization of primordial germ cells in Mongolian sheep

Tissue Maturation in Vivo and in Vitro

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