Transplantation of the gonads of hypogonadal (hpg) mice

Abstract

Testes from hypogonadal (hpg) mice transplanted under the tunica albuginea of the testes of normal mice displayed full spermatogenic activity after 84 days. When ovaries of hpg mice were transplanted to the periovarian capsule of ovariectomized normal females ripe follicles and corpora lutea developed. Although small remnants of normal ovary were found after this operation, the fact that 5 out of 11 normal females bearing transplanted ovaries and mating with heterozygous males raised litters containing hpg mice shows that the ovary of the mutant is capable of producing ova which can be fertilized.

Distribution of 125I-labelled follicle-stimulating hormone and human chorionic gonadotrophin in the gonads of hypogonadal (hpg) mice

“…The present study supports these previous findings, but has also demon¬ strated that a similar pattern of binding is found in hypogonadal mice, with the exception that LH receptors were not found on follicular granulosa cells in the untreated animal used in these studies. This conclusion agrees with previous evidence that the gonads of both hypogonadal males and females possess the capacity for normal development (Bamber et al 1980). It must be noted that FSH receptors could not be detected in the testes of either normal or hypogonadal males.…”

“…The fact that the gonads of both hypogonadal males and females possess the capacity for normal development has been demonstrated by transplanting testes from hypogonadal males beneath the tunica albugínea of normal mice, and ovaries from mutant females into the periovarian capsules of normal females (Bamber, Iddon, Charlton & Ward, 1980). The present studies demonstrate that all the normal mechanisms involved in gonadal responses to follicle-stimulating hormone (FSH) and luteinizing hormone (LH) can be fully developed in hypogonadal mice.…”

Effect of eCG Administration on the Reproductive Function in Hypogonadal (hpg) Male Mice.

“…These results agreed with previous observations in hypophysectomized animals [14]. The duration for these processes and the composition of the germ cells in hpg mice were quite similar to those in normal mice [15], although the response to hormone therapy of the hpg mice was much weaker than that of hypophysectomized mice [5,16,17].…”

Gonadotrophin Release in Hypogonadal and Normal Mice After Electrical Stimulation of the Median Eminence or Injection of Luteinizing Hormone Releasing Hormone

“…DISCUSSION Although the hpg mouse has reduced pituitary stores of gonadotrophins and virtually undetectable hypothalamic LH-RH activity (Cattanach et al 1977), these animals respond to chronic treatment with exogenous synthetic LH-RH with increased growth of the gonads and accessory organs (C. A. Iddon, H. M. Charlton, S. Bamber & G. Fink, unpublished observations). Furthermore, ovaries and testes transplanted to normal mice develop full gametogenic function (Bamber, Iddon, Charlton & Ward, 1980). These findings indicate that the hypogonadism is caused by an abnormality of central origin, probably at the hypothalamic level.…”