Transplantation of the Intact Mammalian Heart

Mann, Frank C.; Priestley, James T.; Markowitz, Jacob; Yater, Wallace M.

doi:10.1001/archsurg.1933.01170020053003

Cited by 196 publications

(41 citation statements)

References 3 publications

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“…When heterotopic cardiac xenotransplantation is performed in this combination, the transplant is functionally identical to the rat (and mouse) allogeneic situation, the vascular supply being provided by the coronary flow alone, according to Mann et al 23 Indeed, blood enters the heterotopically transplanted heart coming from the donor aorta to the graft aorta, and is diverted by the aortic valve to the coronaries. The perfused myocardium drains the blood via the coronary sinus to the right atrium.…”

Section: Discussionmentioning

confidence: 99%

Heterotopic cardiac xenotransplantation in rodents: Report of a refined technique in a hamster‐to‐rat model

et al. 2005

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Hamster-to-rat heterotopic cardiac xenotransplantation is widely used as an experimental model to study xenograft rejection, accommodation, and tolerance, as well as in studies aimed at developing immunosuppressive strategies in xenotransplantation. Despite its widespread application, no detailed description of a surgical technique for this model has been provided in the literature. Indeed, all publications so far on the use of this species combination refer to the rat allotransplantation technique. Hence the present paper provides a detailed, up-to-date description of the surgical method adopted at our center for the hamster-to-rat heterotopic cardiac xenotransplantation model. Considerable effort went into developing a reliable, reproducible experimental model in rodents, and the description given here is enriched with "tips" that we learned in the process. The discussion of the technique also addresses several significant related issues, e.g., the anesthesia and organ preservation solution used (aspects that, in our experience, are crucial to a good surgical outcome).

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Section: Discussionmentioning

confidence: 99%

Heterotopic cardiac xenotransplantation in rodents: Report of a refined technique in a hamster‐to‐rat model

et al. 2005

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“…[27]. 27 mon grel dogs of both sexes (body weight: 8-35 kg) received hearts (weight: 8.5-92 g) from 27 donor dogs (body weight: 2.5-7 kg).…”

Section: Methodsmentioning

confidence: 99%

Heterotopic Allogeneic Heart Transplantation in Dogs

Gams¹,

Bornikoel²,

Hagl³

et al. 1973

Eur Surg Res

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The mean survival time of allogeneic cervical heart transplantations in dogs (6.8 ± 0.8 days) was prolonged by immunosuppressive treatment with azathioprine up to 10.5 ± 1.5 days (p = 0.03) and with horse anti-dog lymphocytic serum up to 16.0 ± 3.6 days (p = 0.02). The ECG voltage recorded daily by epicardial ECG leads decreased constantly in untreated transplants until terminal loss of function, while in all transplants it dropped suddenly 1–2 days before rejection. Some days before and at the time of rejection transient increases of lymphocytes in peripheral venous blood were seen in all dogs, although intravenous ALS injections and azathioprine treatment caused lowered lymphocyte counts. All groups showed a significant and prognostically important drop of peripheral thrombocyte counts between the 5th and the 3rd day before rejection. The activities of CPK, GOT, ALD, and with certain reservations LDH, turned out to be early indicators of rejection, while the activity of GPT was of less and that of MDH of no diagnostic value. Symptoms of liver damage by azathioprine were manifest as increases in activities of ALD, LDH, MDH, GOT and GPT. Increases in LDH and MDH activities may be due to WBC damage caused by ALS treatment.

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“…In our experiments we have used our own modification of the original model of heterotopic cervicle homotransplantation of the heart according to Mann [7], Meisner [8] and Nasseri [9],…”

Section: Methodsmentioning

confidence: 99%