Transplantation of Wharton's jelly mesenchymal stem cells encapsulated with Hydroactive® Gel promotes diabetic wound antifibrotic healing in type 2 diabetic rats

Jiao, Yiren; Chen, Xiaolin; Nong, Baoting; Luo, Mingxun; Niu, Yongxia; Huang, Sunxing; Zhang, Jue; Wei, Aisheng; Huang, Junjiu

doi:10.1039/d2tb01649d

Cited by 4 publications

(3 citation statements)

References 83 publications

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“…[ 9 ] As illustrated in Figure S11 (Supporting Information), the glucose levels were above 11.2 × 10 −3 m for 7 days and eventually exceeded 16.7 × 10 −3 m after the streptozotocin intraperitoneal injection, which validated the success of the diabetic mouse model. [ 29 ] The PVA/SA and PCPS‐gel were applied to the full‐thickness (10 mm in diameter) diabetic wounds in diabetic mice, respectively. Diabetic mice without treatment were evaluated as the control group.…”

Section: Resultsmentioning

confidence: 99%

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Li,

Lu,

Yang

et al. 2024

Adv Healthcare Materials

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Nonhealing diabetic wounds are predominantly attributed to the inhibition of angiogenesis, re‐epithelialization, and extracellular matrix synthesis caused by hypoxia. Although oxygen therapy has demonstrated efficacy in promoting healing, its therapeutic impact remains suboptimal due to unsustainable oxygenation. Here we propose an oxygen‐releasing hydrogel patch embedded with polyethylene glycol‐modified calcium peroxide microparticles, which sustainably releases oxygen for 7 days without requiring any supplementary conditions. The released oxygen effectively promotes cell migration and angiogenesis under hypoxic conditions as validated in vitro. The in vivo tests in diabetic mice models show that the sustainably released oxygen significantly facilitates the synthesis of extracellular matrix, induces angiogenesis, and decreases the expression of inflammatory cytokines, achieving a diabetic wound healing rate of 84.2% on day 7, outperforming the existing oxygen‐releasing approaches. Moreover, the proposed hydrogel patch is designed with porous, soft, antibacterial, biodegradable, and storage stability for 15 days. The proposed hydrogel patch is expected to be promising in clinics treating diabetic wounds.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Li,

Lu,

Yang

et al. 2024

Adv Healthcare Materials

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“…Researchers have employed a range of naturally derived and synthetic materials as biomaterials. Natural materials, such as alginate, 142 chitosan, dextran, 143 gelatin, 144 hyaluronic acid, 145–147 silk fibroin, 148–150 elastin 151 and its monomer tropoelastin 152,153 are favoured due to their natural cell recognition sites and biocompatibility. However, natural biomaterials often exhibit poor mechanical strength, rapid degradation, batch-to-batch variation, and limited availability.…”

Section: Biomaterials For Msc Transplantationmentioning

confidence: 99%

Cellular modifications and biomaterial design to improve mesenchymal stem cell transplantation

Wong

Mehta

et al. 2023

Biomater. Sci.

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“…The methods for RNA extraction and PCR analysis were previously described. 62 Briefly, total RNAs were extracted from BMSCs, TSPCs, and HUVECs using TRIzol reagent (Invitrogen) following the manufacturer's instructions. RT-PCR was performed using PCR Master Mix (SYBR Green; Yeasen).…”

Section: Rna Extraction and Qrt-pcrmentioning

confidence: 99%

Three‐dimensional printed Zn₂SiO₄/sodium alginate composite scaffold with multiple biological functions for tendon‐to‐bone repair

Wang,

Jiao,

Zeng

et al. 2023

MedComm – Biomaterials and Applications

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The intricate nature of the tendon–bone interface poses significant challenges for current surgical methods aimed at repairing tendon–bone interface injuries. Despite notable progress in surgical techniques, these methods continue to grapple with hurdles such as complications and suboptimal healing effects. In this study, we prepared a three‐dimensional‐printed composite scaffold by incorporating bioactive ceramic zinc silicate (Zn2SiO4) into sodium alginate (SA) hydrogel. The physicochemical properties and mechanical strength of the SA hydrogel scaffold composited with Zn2SiO4 (ZnSi/SA) were investigated in vitro. Impressively, the 0.5‐ZnSi/SA scaffold exhibited a sustained release of Zn and Si ions, while exhibiting mechanical properties compatible with tendon–bone interface repair. Moreover, cell viability, cell migration, and osteogenic differentiation assay results showed that 0.5‐ZnSi/SA scaffold facilitated the viability, mobility, and osteogenic differentiation of bone marrow stromal cells. In parallel, assessments of cell viability, cell migration, and tendon differentiation indicated that 0.5‐ZnSi/SA scaffold promoted the viability, migration, and tenogenic differentiation of tendon stem/progenitor cells. Moreover, cell viability, cell migration, and tube formation assay results demonstrated that 0.5‐ZnSi/SA scaffold enhanced the viability, migration rate, and angiogenic performance of human umbilical vein endothelial cells. Collectively, our findings suggest a promising therapeutic avenue employing ZnSi/SA scaffold for tendon–bone interface healing.

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Transplantation of Wharton's jelly mesenchymal stem cells encapsulated with Hydroactive® Gel promotes diabetic wound antifibrotic healing in type 2 diabetic rats

Abstract: Diabetic cutaneous ulcers (DCU) are a complication of diabetes patient, which mostly occurring in foot and causing non-healing diabetic foot ulcer. Mesenchymal stem cells (MSCs)-based therapy is currently being investigated...

Cited by 4 publications

References 83 publications

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Cellular modifications and biomaterial design to improve mesenchymal stem cell transplantation

Three‐dimensional printed Zn₂SiO₄/sodium alginate composite scaffold with multiple biological functions for tendon‐to‐bone repair

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Transplantation of Wharton's jelly mesenchymal stem cells encapsulated with Hydroactive® Gel promotes diabetic wound antifibrotic healing in type 2 diabetic rats

Abstract: Diabetic cutaneous ulcers (DCU) are a complication of diabetes patient, which mostly occurring in foot and causing non-healing diabetic foot ulcer. Mesenchymal stem cells (MSCs)-based therapy is currently being investigated...

Cited by 4 publications

References 83 publications

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Calcium Peroxide‐Based Hydrogel Patch with Sustainable Oxygenation for Diabetic Wound Healing

Cellular modifications and biomaterial design to improve mesenchymal stem cell transplantation

Three‐dimensional printed Zn2SiO4/sodium alginate composite scaffold with multiple biological functions for tendon‐to‐bone repair

Contact Info

Product

Resources

About

Three‐dimensional printed Zn₂SiO₄/sodium alginate composite scaffold with multiple biological functions for tendon‐to‐bone repair