Transplante de células-tronco epiteliais límbicas alógenas expandidas ex vivo sobre membrana amniótica: relato de caso

Gomes, José Álvaro Pereira; Pazos, Henrique Santiago Baltar; Silva, André Berger Emiliano; Cristovam, Priscila Cardoso; Júnior, Rubens Belfort

doi:10.1590/s0004-27492009000200025

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…Although less frequently applied in children, amniotic membrane was a useful option here to revert chronic epithelial defect. Moreover, it could also be considered as a carrier of limbal stem cells, as recently reported (9)(10) . The results remain the same until the moment when 8 months of follow-up was completed.…”

Section: Discussionmentioning

confidence: 97%

Ichthyosis follicularis, alopecia and photophobia syndrome (IFAP): report of the first case with ocular and cutaneous manifestations in Brazil with a favorable response to treatment

Höpker

Ribeiro

Oliveira

et al. 2011

Arq. Bras. Oftalmol.

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Section: Discussionmentioning

confidence: 97%

Ichthyosis follicularis, alopecia and photophobia syndrome (IFAP): report of the first case with ocular and cutaneous manifestations in Brazil with a favorable response to treatment

Höpker

Ribeiro

Oliveira

et al. 2011

Arq. Bras. Oftalmol.

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“…Only seven clinical studies (substudies excluded) involving transplantation of ex vivo cultured LEC have applied intact AM (Table ) as a culture substrate, whereas 29 clinical studies used denuded AM to culture LEC (Table ).…”

Section: Culture Techniques and Use Of Intact And Denuded Ammentioning

confidence: 99%

“…The remaining studies using denuded AM as a culture substrate for LEC used both allogeneic and autologous explants, with and without the use of a 3T3 fibroblast feeder layer or air‐lifting (Table ). Immunosuppression was used in all studies using allogeneic limbal explants except for one , and in some studies using autologous explants (Table ).…”

Section: Culture Techniques and Use Of Intact And Denuded Ammentioning

confidence: 99%

Concise Review: Altered Versus Unaltered Amniotic Membrane as a Substrate for Limbal Epithelial Cells

Utheim

Salvanos

et al. 2018

Stem Cells Translational Medicine

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Limbal stem cell deficiency (LSCD) can result from a variety of corneal disorders, including chemical and thermal burns, infections, and autoimmune diseases. The symptoms of LSCD may include irritation, epiphora, blepharospasms, photophobia, pain, and decreased vision. There are a number of treatment options, ranging from nonsurgical treatments for mild LSCD to various forms of surgery that involve different cell types cultured on various substrates. Ex vivo expansion of limbal epithelial cells (LEC) involves the culture of LEC harvested either from the patient, a living relative, or a cadaver on a substrate in the laboratory. Following the transfer of the cultured cell sheet onto the cornea of patients suffering from LSCD, a successful outcome can be expected in approximately three out of four patients. The phenotype of the cultured cells has proven to be a key predictor of success. The choice of culture substrate is known to affect the phenotype. Several studies have shown that amniotic membrane (AM) can be used as a substrate for expansion of LEC for subsequent transplantation in the treatment of LSCD. There is currently a debate over whether AM should be denuded (i.e., de‐epithelialized) prior to LEC culture, or whether this substrate should remain intact. In addition, crosslinking of the AM has been used to increase the thermal and mechanical stability, optical transparency, and resistance to collagenase digestion of AM. In the present review, we discuss the rationale for using altered versus unaltered AM as a culture substrate for LEC. Stem Cells Translational Medicine 2018;7:415–427

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“…Neste mesmo ano foi apresentado o primeiro relato de caso acerca de um paciente com baixa acuidade visual progressiva e irritação ocular em ambos os olhos há sete anos. O paciente apresentava exotropia desde a infância, tendo sido submetido à cirurgia para correção quando tinha sete anos (GOMES et al, 2009).…”

Section: Uso Clínico Na Oftalmologiaunclassified

Uso De Terapia Celular Com Células-Tronco Nas Especialidades Médicas No Brasil: Revisão Sistemática

Barbosa¹,

Menezes²,

Emanuelli³

2018

Rev. Uningá

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O uso de células-tronco na medicina demonstra inúmeras possibilidades terapêuticas, entretanto, não há resultados a longo prazo destes tratamentos até o momento, gerando incerteza quanto à sua utilização, tanto na comunidade médica e para os pacientes. Um estudo que apresente sistematicamente o estado da arte do uso clínico destas células seria esclarecedor tanto para o meio médico quanto para os pacientes. Diante disso, este trabalho tem por objetivo descrever as aplicações clínicas disponíveis no Brasil para o uso de células-tronco em diferentes especialidades médicas. Realizou-se uma revisão sistemática de caráter exploratório da literatura presente nas bases de dados Lilacs, Ibecs, Medline e Scielo, com os descritores Células-tronco, Medicina regenerativa, Terapia baseada em transplante de células e tecidos e Transplante de células-tronco. Foram selecionados 20 artigos de acordo com o cumprimento do objetivo e critérios metodológicos, abrangendo ás áreas médicas da hematologia, cardiologia, oftalmologia, reumatologia, ortopedia, cirurgia vascular e dermatologia. Conclui-se que o tratamento com células-tronco é empregado de forma definitiva ou adjuvante a outras terapias nas áreas médicas supracitadas, diminuindo em alguns casos o emprego de terapias mais agressivas ou a minimização dos efeitos colaterais associados a estas, além de trazer resultados que não poderiam ser conseguidos anteriormente.

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Transplante de células-tronco epiteliais límbicas alógenas expandidas ex vivo sobre membrana amniótica: relato de caso

Cited by 8 publications

References 11 publications

Ichthyosis follicularis, alopecia and photophobia syndrome (IFAP): report of the first case with ocular and cutaneous manifestations in Brazil with a favorable response to treatment

Ichthyosis follicularis, alopecia and photophobia syndrome (IFAP): report of the first case with ocular and cutaneous manifestations in Brazil with a favorable response to treatment

Concise Review: Altered Versus Unaltered Amniotic Membrane as a Substrate for Limbal Epithelial Cells

Uso De Terapia Celular Com Células-Tronco Nas Especialidades Médicas No Brasil: Revisão Sistemática

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