Transplantion of predominant Lactobacilli from native hens to commercial hens could indirectly regulate their ISC activity by improving intestinal microbiota

Abstract

Summary In poultry, HyLine (HL) Hens are known for their excellent laying performance. However, ZhenNing (ZN) Hens, a native chicken breed in China, are known for their unique flavour. The intestinal mucosa, which is the main organ for nutrient absorption, could affect livestock product quality. In ZN Hens’ intestinal mucosa, we found more villus wrinkles, larger villus circumference and higher amino acid transporters mRNA abundance compared with HL Hens. Among three laying periods of ZN Hens, in the intestinal lumen, Lactobacillus salivarius (L. sa.), Lactobacillus agilis (L. ag.) and Lactobacillus aviarius were the predominant species in the laying peak period. Furthermore, multiple‐antibiotics feeding in ZN Hens and predominant Lactobacillus feeding in HL Hens suggested that these Lactobacilli could indeed increase villus wrinkles and improve intestinal absorption. In HL Hens, L. sa. + L. ag. treatment could promote organoids budding in vitro, and promote epithelial proliferation in vivo. Collectively, the unique intestinal mucosa morphology in ZN Hens was due to the high abundance of intestinal L. sa. and L. ag. Transplant these Lactobacilli to HL Hens could increase their intestinal probiotics abundance, fine adjust the intestinal stem cell function and promote the epithelial proliferation, in turn, increase villus winkles and mucosal absorption area.

Lactobacillus salivarius Promotion of Intestinal Stem Cell Activity in Hens Is Associated with Succinate-Induced Mitochondrial Energy Metabolism

“…In our previous study, cofeeding of L. salivarius and L. agilis in laying hens was demonstrated to decrease the ISCs differentiation ( 11 ). Currently, after the administration of Lactobacillus component (supernatant, precipitation) on hens individually or combined, the LGR5 protein expression in vivo was downregulated, the mRNA abundance of amino acid/peptide transporters presented a similar alteration trend.…”

“…Isolation of chicken’s intestinal crypt was performed based on the protocol previously published ( 11 , 12 ). Briefly, the duodenum was collected from HL hens at D280 (laying peak period) sterility, cut into 2–4 mm 3 pieces and shaken gently in 2 mM cold EDTA (ethylenediaminetetraacetic acid, pH 7.4).…”

“…The quantification Real-Time PCR (qPCR) or RT-PCR were performed base on the protocol previously published ( 11 ). Briefly, total RNA from the intestinal tissue or organoids were extracted using TRIzol reagent and reverse transcribed into cDNA.…”

“…Lactobacillus has been widely used in the poultry industry to ameliorate egg-laying performance and egg quality. In our previous studies, predominant Lactobacilli ( L. salivarius and L. agilis ), which were screened from native hens and fed in HyLine hens, were demonstrated to improve egg-laying rate, egg weight, and albumen’s amino acid levels ( 11 , 12 ). Similarly, L. salivarius CML352 (isolated from Chinese native chicken) and L. agilis were proved to improve egg quality or increase weight gains in chicken ( 13 , 14 ), feeding of L. plantarum or cofeeding of L. pentosus ITA23 and L. acidophilus ITA44 were proved to strengthen intestinal mucosal absorption ( 15 , 16 ).…”

Lactobacillus Plantarum injection at the embryonic stage alters the early growth performance and lipid metabolism of broilers by specific genera of bacteria

“…In addition, commensal organisms contribute to the colonization and function of lactobacilli via cross-feeding. Lactobacillus has been demonstrated to mitigate inflammation through its surface components and metabolites, thereby enhancing the intestinal epithelial barrier function ( Liu et al, 2022 ). Interactions between the ligands from lactobacilli and immunomodulatory receptors are implicated in immunomodulation ( Huang et al, 2022 ).…”