Transport and Toxicity of Silver Nanoparticles in HepaRG Cell Spheroids

Senyavina, N. V.; Gerasimenko, T. N.; Pulkova, N. V.; Maltseva, D. V.

doi:10.1007/s10517-016-3321-6

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…Cells in a 3D system tolerate higher silver concentrations than in a 2D system, which further supports the probability of success of such a material as coating for prosthetic joints. 65,66 In an in vivo setting rather than in cell cultures, the therapeutic window may therefore be improved.…”

Section: Discussionmentioning

confidence: 99%

Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria

Hérault

Wagner

Abram

et al. 2020

IJN

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Background: Joint arthroplasty has improved the quality of life of patients worldwide, but infections of the prosthesis are frequent and cause significant morbidity. Antimicrobial coatings for implants promise to prevent these infections. Methods: We have synthesized nanocapsules of titanium dioxide in amorphous or anatase form containing silver as antibacterial agent and tested their impact on bacterial growth. Furthermore, we explored the possible effect of the nanocapsules on the immune system. First, we studied their uptake into macrophages using a combination of electron microscopy and energy-dispersive spectroscopy. Second, we exposed immune cells to the nanocapsules and checked their activation state by flow cytometry and enzyme-linked immunosorbent assay. Results: Silver-containing titanium dioxide nanocapsules show strong antimicrobial activity against both E. coli and S. aureus and even against a multidrug-resistant strain of S. aureus. We could demonstrate the presence of the nanocapsules in macrophages, but, importantly, the nanocapsules did not affect cell viability and did not activate proinflammatory responses at doses up to 20 μg/mL. Conclusion: Our bactericidal silver-containing titanium dioxide nanocapsules fulfill important prerequisites for biomedical use and represent a promising material for the coating of artificial implants.

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Section: Discussionmentioning

confidence: 99%

Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria

Hérault

Wagner

Abram

et al. 2020

IJN

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“…Rather, ROS production following minimal cellular insult induces mast cell activation. Silver NPs can either damage cell membranes or enter cells by diffusion for some cell types, including endothelial cells or hepatocytes, and by endocytosis in phagocytic cells . The K + efflux followed by membrane damage may trigger histamine release from mast cells directly or indirectly via the production of intracellular ROS .…”

Section: Resultsmentioning

confidence: 99%

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

Kang

Kim

Lee

et al. 2016

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The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.

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“…Although many in vitro studies focusing on NP-induced toxicity used 2D cultivated cells, recently 3D microtissues have been used in this context. 37,39,67 Nanosilica have been described to induce cellular oxidative stress and to cause inflammation and DNA damage leading to cell death in various 2D cell types. 68,69 Landgraf et al reported only weak biological effects of SiO 2 NPs on HepG2 cells.…”

Section: Cytotoxicity Of Sio 2 Nps In 3d Liver Microtissuesmentioning

confidence: 99%

“…32,35 In recent years, liver microtissues were used as a model for NP toxicity assessment. 34,[36][37][38][39] Dubiak-Szepietowska et al observed a stronger cytotoxic effect of Ag, ZnO, and SiO 2 NPs on HepG2 cells when grown in 2D compared to cells grown in 3D hydrogels. 34 The lower sensitivity to toxic concentrations of Ag NPs was also detected in HepaRG cell spheroids.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of SiO2 nanoparticles in 3D liver microtissues

Fleddermann

Susewind²,

Peuschel

et al. 2019

IJN

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Introduction: Nanoparticles (NPs) are used in numerous products in technical fields and biomedicine; their potential adverse effects have to be considered in order to achieve safe applications. Besides their distribution in tissues, organs, and cellular localization, their impact and penetration during the process of tissue formation occurring in vivo during liver regeneration are critical steps for establishment of safe nanomaterials. Materials and methods: In this study, 3D cell culture of human hepatocarcinoma cells (HepG2) was used to generate cellular spheroids, serving as in vitro liver microtissues. In order to determine their differential distribution and penetration depth in HepG2 spheroids, SiO 2 NPs were applied either during or after spheroid formation. The NP penetration was comprehensively studied using confocal laser scanning microscopy and scanning electron microscopy. Results: Spheroids were exposed to 100 µg mL −1 SiO 2 NPs either at the beginning of spheroid formation, or during or after formation of spheroids. Microscopy analyses revealed that NP penetration into the spheroid is limited. During and after spheroid formation, SiO 2 NPs penetrated about 20 µm into the spheroids, corresponding to about three cell layers. In contrast, because of the addition of SiO 2 NPs simultaneously to cell seeding, NP agglomerates were located also in the spheroid center. Application of SiO 2 NPs during the process of spheroid formation had no impact on final spheroid size. Conclusion: Understanding the distribution of NPs in tissues is essential for biomedical applications. The obtained results indicate that NPs show only limited penetration into already formed tissue, which is probably caused by the alteration of the tissue structure and cell packing density during the process of spheroid formation.

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Transport and Toxicity of Silver Nanoparticles in HepaRG Cell Spheroids

Cited by 9 publications

References 14 publications

<p>Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria</p>

<p>Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria</p>

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

<p>Distribution of SiO<sub>2</sub> nanoparticles in 3D liver microtissues</p>

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Transport and Toxicity of Silver Nanoparticles in HepaRG Cell Spheroids

Cited by 9 publications

References 14 publications

<p>Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria</p>

<p>Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria</p>

5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells

<p>Distribution of SiO<sub>2</sub>&nbsp;nanoparticles&nbsp;in 3D liver microtissues</p>

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<p>Distribution of SiO<sub>2</sub> nanoparticles in 3D liver microtissues</p>