Transport of a synaptotagmin–YFP fusion protein in sympathetic neurons during early neurite outgrowth in vitro after transfection in vivo

Narayan, Sujatha; Greif, Karen F.

doi:10.1016/j.jneumeth.2003.10.002

Cited by 4 publications

(5 citation statements)

References 42 publications

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“…The second one corresponds to the TCC1 neurons and the fast propagation of this TCC over the OT surface (DS2‐DS3/ED4‐ED6). These neurons first accumulate at the PMZ and begin the expression of βIIITub and Syt, indicating the onset of somal differentiation and axonogenesis (Moody et al,1989; Littleton et al,1995; Haendel et al,1996; Molea et al,1999; Bergmann et al,1999,2000; Narayan and Greif,2004). The earliest and the latest of these neurons will originate the SGC and the SGP neurons, respectively.…”

Section: Discussionmentioning

confidence: 99%

The chick optic tectum developmental stages. A dynamic table based on temporal‐ and spatial‐dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis

Rapacioli¹,

Celin²,

Duarte³

et al. 2011

Journal of Morphology

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Development is often described as temporal sequences of developmental stages (DSs). When tables of DS are defined exclusively in the time domain they cannot discriminate histogenetic differences between different positions along a spatial reference axis. We introduce a table of DSs for the developing chick optic tectum (OT) based on time- and space-dependent changes in quantitative morphometric parameters, qualitative histogenetic features and immunocytochemical pattern of several developmentally active molecules (Notch1, Hes5, NeuroD1, β-III-Tubulin, synaptotagmin-I and neurofilament-M). Seven DSs and four transitional stages were defined from ED2 to ED12, when the basic OT cortical organization is established, along a spatial developmental gradient axis extending between a zone of maximal and a zone of minimal development. The table of DSs reveals that DSs do not only progress as a function of time but also display a spatially organized propagation along the developmental gradient axis. The complex and dynamic character of the OT development is documented by the fact that several DSs are simultaneously present at any ED or any embryonic stage. The table of DSs allows interpreting how developmental cell behaviors are temporally and spatially organized and explains how different DSs appear as a function of both time and space. The table of DSs provides a reference system to characterize the OT corticogenesis and to reliably compare observations made in different specimens.

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Section: Discussionmentioning

confidence: 99%

The chick optic tectum developmental stages. A dynamic table based on temporal‐ and spatial‐dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis

Rapacioli¹,

Celin²,

Duarte³

et al. 2011

Journal of Morphology

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“…E8 chick forebrain neurons were infected either at the time of plating or 18 to 24 h after plating with syt1‐YFP constructs controlled by the constitutive CMV promoter using adenoviral mediated transfer (Narayan and Greif,2004). We added adenovirus containing either the syt1‐YFP construct or a control GFP construct (QBiogene) at a MOI of 100 to 200.…”

Section: Methodsmentioning

confidence: 99%

“…Syt1 is targeted to axon terminals by sequences in its C‐terminal domain (Krasnov and Enikopolov,2000; Adolfsen et al, 2004). Since syt1 is expressed in many developing systems during axon growth before synapse formation (Greif and Reichardt,1982; Chun and Shatz,1988; Lou and Bixby, 1995; Lin et al,1996) and is targeted throughout axons (Adolfsen et al,1994; Basarsky et al,1994; Kraszewski et al,1995; Nakata et al,1998; Krasnov et al,2000; Narayan and Greif,2004; Gumy et al,2011), it may have a role in axon extension and branching.…”

Section: Introductionmentioning

confidence: 99%

Synaptotagmin‐1 promotes the formation of axonal filopodia and branches along the developing axons of forebrain neurons

Greif

Asabere

Lutz

et al. 2012

Developmental Neurobiology

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Synaptotagmin-1 (syt1) is a Ca(2+)-binding protein that functions in regulation of synaptic vesicle exocytosis at the synapse. Syt1 is expressed in many types of neurons well before synaptogenesis begins both in vivo and in vitro. To determine if expression of syt1 has a functional role in neuronal development before synapse formation, we examined the effects of syt1 overexpression and knockdown on the growth and branching of the axons of cultured primary embryonic day 8 chicken forebrain neurons. In vivo these neurons express syt1, and most have not yet extended axons. We present evidence that syt1 plays a role in regulating axon branching, while not regulating overall axon length. To study the effects of overexpression of syt1, we used adenovirus-mediated infection to introduce a syt1-YFP construct, or control GFP construct, into neurons. Syt1 levels were reduced using RNA interference. Overexpression of syt1 increased the formation of axonal filopodia and branches. Conversely, knockdown of syt1 decreased the number of axonal filopodia and branches. Time-lapse analysis of filopodial dynamics in syt1-overexpressing cells demonstrated that elevation of syt1 levels increased both the frequency of filopodial initiation and their lifespan. Taken together these data indicate that syt1 regulates the formation of axonal filopodia and branches before engaging in its conventional functions at the synapse.

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“…A utilização de genes marcadores, que codificam para proteínas fluorescentes, apresentam muitas vantagens, pois podem ser detectadas "in situ" em células isoladas, mesmo que localizadas em sistemas complexos, usando microscópio de fluorescência (Normander et al, 1999) e a fluorescência é independente da presença de substrato, apesar de que para a expressão adequada da proteína é necessário a presença de oxigênio (Heim et al, 1994). Griesbeck et al (2001) Vários outros estudos foram realizados utilizando a expressão do gene yfp como, por exemplo, em neurônios de rato (Narayan & Greif, 2004), em células de humanos (Kretzschmar et al, 2004, Sorkina et al, 2003, em células de plantas como de…”

Section: Discussionunclassified

“…A expressão do gene yfp tem sido utilizada para estudos com microorganismos (Griesbeck et al, 2001, Miller et al, 2000, Stuurman et al 2000, células de plantas (Nowak et al, 2004, Tse et al, 2004, Bolte et al, 2004, de animais (Narayan & Greif, 2004), e em células de humanos (Kretzschmar et al, 2004, Sorkina et al, 2003).…”

Section: O Genoma De Mais De 100 Raças De Bactérias Está Completamentunclassified

Disrupção das ORFs XF-810, XF-818, XF-1940, XF-2359 e XF-2708 de >i<Xylella fastidiosa>/i<-CVC possivelmente relacionadas à patogenicidade da bactéria

Gonzalez¹

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Página Linha Onde se lê: Leia-se: xvi 11 ... primer "reverse" que é complementar à .. .primer "reverse" que é complementar à porção 3' do gene porção 3' de uma seqüência de DNA xix 7 Variegated Clorose ofthe citros ... Citrus Variegated Chlorosis ... 4 24 ... gram-negativas ... ... Gram-negativas ... 7 12 ... Lopes et ai. (1999) em São Paulo ... ... Lopes et ai. (1999) nas análises realizadas com amostras do estado de São Paulo ... 8 ... encurtamento dos intemódios ... ... encurtamento dos entrenós ... 8 21 ... Este bloqueio dos vasos pelas células ... Sugeriu-se que este bloqueio dos vasos bacterianas é associado com alterações pelas células bacterianas pode estar fisiológicas ... associado com alterações :fisiológicas ... 9 15 ... para detectar raça de X fastidiosa .... .. para detectar linhagem de X fastidiosa ... 12 ... ela é incapaz de crescer mais rápido ... ... apresenta baixa velocidade de replicação celular ... 12 ... mas nutricionalmente diluído ... ... mas nutricionalmente mais pobre ... 15 ... Na planta, a adesão na parede do ... Acredita-se que na planta a adesão na xilema ... parede do xilema ... 16 9 ... 100 raças de bactérias .... .. 100 linhagens,de bactérias ... 19 .. Actinobacillus ... .. Actinobacillus sp ... 19 ... um pedaço desses genes ... ... fragmentos desses genes ... 20 ... FimA-e FimP-, estes mutantes ... ... FimA-e FimF-analisado "in vitro". Estes mutantes ... 21 ... que apesar de ideal é inapropriada, está ... está no fato de que o desenvolvimento no fato de que o desenvolvimento dos dos sintomas ... sintomas ... 24 ... é restrito às membranas ... ... é restrito às membranas de pontuação ... 28 3 ... exoplissacarídeos ... ... exopolissacarídeos ... 28 ... MsrA de raças ... ... MsrA de linhagens ... 36 A transformação dos produtos de ligação ... Os produtos de ligação foram transformados ...

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Transport of a synaptotagmin–YFP fusion protein in sympathetic neurons during early neurite outgrowth in vitro after transfection in vivo

Cited by 4 publications

References 42 publications

The chick optic tectum developmental stages. A dynamic table based on temporal‐ and spatial‐dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis

The chick optic tectum developmental stages. A dynamic table based on temporal‐ and spatial‐dependent histogenetic changes: A structural, morphometric and immunocytochemical analysis

Synaptotagmin‐1 promotes the formation of axonal filopodia and branches along the developing axons of forebrain neurons

Disrupção das ORFs XF-810, XF-818, XF-1940, XF-2359 e XF-2708 de >i<Xylella fastidiosa>/i<-CVC possivelmente relacionadas à patogenicidade da bactéria

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