Transport of living cells with magnetically assembled nanowires

Choi, Daniel S.; Fung, Andrew O.; Moon, Hyejin; Ho, Dean; Chen, Y.; Kan, Eunsung; Rheem, Youngwoo; Yoo, B-Y; Myung, Nosang V.

doi:10.1007/s10544-006-9008-4

Cited by 37 publications

(32 citation statements)

References 6 publications

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“…54 In the case of Ni NWs, the surface charge (almost neutral) is favorable to internalization efficacy, which under the conditions tested here was rather high, given that some NWs were found to be fully internalized after only 1 hour of incubation. Similar results were previously reported for thick Ni NWs (200 nm diameter) that were observed to cross the cell membrane after 40 minutes of incubation with rat neuroblastoma cells 17 and for thin To analyze the uptake and intracellular fate of Ni NWs, colon cancer cells incubated with Ni NWs were fixed, carefully sliced, and imaged by TEM. Two cell samples were prepared and imaged in replicate: a control sample (no NWs added) and a sample of cells incubated with Ni NWs.…”

Section: Magnetic Nws-cells Interactionsupporting

confidence: 82%

“…Using direct current fields, MNPs can be trapped, concentrated, [11][12][13][14] or used in cell separation. [15][16][17] Under alternating fields, MNPs can be heated 18 or rotated, 19,20 and, in cases of elongated structures, they can transmit forces or torques to whatever they are in contact with.…”

Section: Introductionmentioning

confidence: 99%

“…Out of these two, Fe NWs tend to aggregate more, since they have a higher remanence magnetization value, 23 making Ni a better material in that regard. Furthermore, Ni NWs have proven to be efficient in cell separation, manipulation, and purification 16,17,21,22,[24][25][26] as well as in delivering cargoes of various macro-particles including biological entities. 27 Recent studies have investigated their use as therapeutic agents for hyperthermia 28 and cell inflammation induction 29 in cultures of human embryonic cells.…”

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confidence: 99%

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Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

102

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In this paper, we show that magnetic nanowires with weak magnetic fields and low frequencies can induce cell death via a mechanism that does not involve heat production. We incubated colon cancer cells with two concentrations (2.4 and 12 μg/mL) of nickel nanowires that were 35 nm in diameter and exposed the cells and nanowires to an alternating magnetic field (0.5 mT and 1 Hz or 1 kHz) for 10 or 30 minutes. This low-power field exerted a force on the magnetic nanowires, causing a mechanical disturbance to the cells. Transmission electron microscopy images showed that the nanostructures were internalized into the cells within 1 hour of incubation. Cell viability studies showed that the magnetic field and the nanowires separately had minor deleterious effects on the cells; however, when combined, the magnetic field and nanowires caused the cell viability values to drop by up to 39%, depending on the strength of the magnetic field and the concentration of the nanowires. Cell membrane leakage experiments indicated membrane leakage of 20%, suggesting that cell death mechanisms induced by the nanowires and magnetic field involve some cell membrane rupture. Results suggest that magnetic nanowires can kill cancer cells. The proposed process requires simple and low-cost equipment with exposure to only very weak magnetic fields for short time periods.

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Section: Magnetic Nws-cells Interactionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

102

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“…The energies of the 3d and 4s orbitals in the neutral atoms are quite similar, and their configurations are 3dn4s2 except for Cu (3d104s1), which is attributed to the stability of the filled d shell. In addition, cobalt containing compounds recently attracted considerable interest as systemic anticancer agents [12], while nickel (II) is vital for living beings [13] and it has been reported that Ni has apoptogenic capabilities [14].…”

Section: Introductionmentioning

confidence: 99%

Can metal replacement increase the antitumor activity of azurin? (in vitro study)

Ali¹,

Mohamed²,

Sedek³

2017

Gen Med Open

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Azurin is a member of blue-copper proteins family which induces apoptosis in cancer cells. In the present work, azurin (Az) has been modified via replacement of copper at active site with cobalt and nickel. FTIR revealed that its secondary structure is practically indistinguishable from that of its derivatives. UV spectroscopy and DSC results revealed that native form of Az is more stable than Co-and Ni forms. SRB was used to elucidate their possible anti-tumor effect on cancer cell lines from breast and colon cells (MCF-7 and HCT116, respectively). To our best knowledge, this is the first time metal replacement of Az has been used with cell line. It appears that this process is the predominant reason for the effectiveness of Az. Experimental results showed that the anti-tumor activity of Cu-and Co Az (at concentration ≤ 25 µg/L) is higher for colon-relative to breast cells, while that for Ni-Az is higher for both cells (at low concentration, 5 µg/L). Hence, according to our findings it was revealed that Ni-Az form has the most extensive anti-antitumor effect on breast-and colon cancer cells. This highlights that Ni may be bona fide copper ion impersonator in vivo.

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“…Among the broad range of nanomaterials that are currently being investigated for bioapplications, one dimensional (1D) nanostructures have received special attention due to their variable aspect ratio leading to tunable properties [5][6][7]. In particular, nickel nanowires (NiNW) exhibit ferromagnetic properties that allow easy magnetic manipulation at low external magnetic fields, thus appearing as good candidates for several biomedical applications, including cell manipulation and separation [8][9][10][11][12][13][14][15][16][17][18][19][20]. According to reports on comparative studies of magnetic cell separation involving spheroidal nanoparticles and nanowires, the latter systems seem more efficient due to their magnetic anisotropy and strong magnetic moment [8,12,13,17,21,22].…”

Section: Introductionmentioning

confidence: 99%

Functionalization of nickel nanowires with a fluorophore aiming at new probes for multimodal bioanalysis

Pinheiro

Sousa

Araújo

et al. 2013

Journal of Colloid and Interface Science

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This work reports research on the development of bimodal magnetic and fluorescent 1D nanoprobes. First, ferromagnetic nickel nanowires (NiNW) have been prepared by Ni electrodeposition in anodic aluminum oxide (AAO) template. The highly ordered self-assembled AAO nanoporous templates were fabricated using a two-step anodization method of aluminum foil. The surface of the NiNW were then modified with polyethyleneimine (PEI) which was previously labeled with an organic dye (fluorescein isothiocyanate: FITC) via covalent bonding. The ensuing functionalized NiNW exhibited the characteristic green fluorescence of FITC and could be magnetically separated from aqueous solutions by using a NdFeB magnet. Finally, the interest of these bimodal NiNW as nanoprobes for in vitro cell separation and biolabeling was preliminary assessed in a proof of principle experiment that involved the attachment of biofunctionalized NiNW to blood cells.

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Transport of living cells with magnetically assembled nanowires

Cited by 37 publications

References 6 publications

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Can metal replacement increase the antitumor activity of azurin? (in vitro study)

Functionalization of nickel nanowires with a fluorophore aiming at new probes for multimodal bioanalysis

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