Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis

Rayens, Nathan T; Cook, Keisha; McKinley, Scott A.; Payne, Christine K.

doi:10.1016/j.bpj.2022.02.032

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…Lysosomes in the perinuclear region exhibit also reduced mobility compared with those in the periphery. This diminished transport within the perinuclear domain may be due to variances in cytoskeletal connections within this region, an effect of progressive lysosomal maturation state, or underscore the emerging role of the endoplasmic reticulum-lysosome contact sites (Cabukusta and Neefjes, 2018;Rayens et al, 2022). Various signals or conditions affect lysosome positioning.…”

Section: Hämälistö Et Almentioning

confidence: 99%

Endolysosomal vesicles at the center of B cell activation

Hämälistö,

Del Valle Batalla,

Yuseff

et al. 2024

Journal of Cell Biology

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The endolysosomal system specializes in degrading cellular components and is crucial to maintaining homeostasis and adapting rapidly to metabolic and environmental cues. Cells of the immune system exploit this network to process antigens or promote cell death by secreting lysosome-related vesicles. In B lymphocytes, lysosomes are harnessed to facilitate the extraction of antigens and to promote their processing into peptides for presentation to T cells, critical steps to mount protective high-affinity antibody responses. Intriguingly, lysosomal vesicles are now considered important signaling units within cells and also display secretory functions by releasing their content to the extracellular space. In this review, we focus on how B cells use pathways involved in the intracellular trafficking, secretion, and function of endolysosomes to promote adaptive immune responses. A basic understanding of such mechanisms poses an interesting frontier for the development of therapeutic strategies in the context of cancer and autoimmune diseases.

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Section: Hämälistö Et Almentioning

confidence: 99%

Endolysosomal vesicles at the center of B cell activation

Hämälistö,

Del Valle Batalla,

Yuseff

et al. 2024

Journal of Cell Biology

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Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility

Rayens

Cook

McKinley

et al. 2023

Biophysical Journal

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Neurons and astrocytes have distinct organelle signatures and responses to stress

Rhoads,

Dong,

Hsu

et al. 2024

Preprint

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Neurons and astrocytes play critical yet divergent roles in brain physiology and neurological conditions. Intracellular organelles are integral to cellular function. However, an in-depth characterization of organelles in live brain cells has not been performed. Here, we used multispectral imaging to simultaneously visualize six organelles - endoplasmic reticulum (ER), lysosomes, mitochondria, peroxisomes, Golgi, and lipid droplets - in live primary rodent neurons and astrocytes. We generated a dataset of 173 Z-stack and 99 time-lapse images accompanied by quantitative analysis of 1418 metrics (the "organelle signature"). Comparative analysis revealed clear cell-type speciﬁcity in organelle morphology and interactions. Neurons were characterized by prominent mitochondrial composition and interactions, while astrocytes contained more lysosomes and lipid droplet interactions. Additionally, neurons displayed a more robust organelle response than astrocytes to acute oxidative or ER stress. Our data provide a systems-level characterization of neuron and astrocyte organelles that can be a reference for understanding cell-type-speciﬁc physiology and disease.

show abstract

Transport of lysosomes decreases in the perinuclear region: Insights from changepoint analysis

Cited by 5 publications

References 45 publications

Endolysosomal vesicles at the center of B cell activation

Endolysosomal vesicles at the center of B cell activation

Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility

Neurons and astrocytes have distinct organelle signatures and responses to stress

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