Transport of monosaccharides in kidney-cortex cells

Kleinzeller, A.; Kolínská, J; Beneš, I

doi:10.1042/bj1040852

Cited by 94 publications

(33 citation statements)

References 16 publications

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“…A reduction of the external Na+ concentration did not change the apparent Km of the carrier for metaraminol but lowered the apparent Vmax (Sugrue & Shore, 1969b). Na+ had a similar effect on the transport of sugars by rabbit kidney slices (Kleinzeller, Kolinska & Benes, 1967) and by rabbit ileum (Goldner, Schultz & Curran, 1969) but a reduction in Na+ altered the Km and left the Vm.,, unchanged for sugar transport by hamster intestine (Crane, Forstner & Eichholz, 1965) and for amino-acid transport by rabbit lymph node cells (Kipnis & Parrish, 1965). It is possible that the adrenergic neurone membrane transport site for noradrenaline and metaraminol combines with both Na+ and amine to form a ternary complex.…”

Section: Discussionmentioning

confidence: 99%

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Paton

1971

British J Pharmacology

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4. The inhibitory effects on transport of either a marked reduction in the concentration of Na+ or of omission of K+ were rapidly reversible on exposure of slices to Krebs solution. 5. The inhibitory effect of ouabain on [3H]-metaraminol transport was markedly time dependent, being significantly increased by preincubation with ouabain. The inhibitory action of ouabain was not affected, however, by the Na+ concentration present during preincubation. 6. An inwardly directed Na+ gradient did not increase [3H]-metaraminol transport in slices in which Na+ pumiping had been prevented by 1O-5M ouabain, 40 C, omission of K+ or by metabolic inhibition. 7. These findings provide additional evidence that the Na+ and K+ activated adenosine triphosphatase may participate in the transport of metaraminol and related amines. In the absence of Na+ pumping, induced Na+ gradients were unable to produce transport of amine as would be predicted by the co-transport model proposed by . IntroductionTissues accumulate noradrenaline and structurally related compounds such as metaraminol, by a process which is temperature dependent, requires energy, is Na+ and K+ dependent and is inhibited by ouabain (Dengler, Michaelson, Spiegel &

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Section: Discussionmentioning

confidence: 99%

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Paton

1971

British J Pharmacology

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“…Studies in the rabbit indicate that 2-deoxyglucose enters renal tubular cells (20) and studies in the rat show that after phosphorylation, 2-deoxyglucose inhibits glycolysis at the phosphoglucose isomerase step (21). In Ehrlich ascites tu-mor cells (6, 10), 2-deoxyglucose is phosphorylated by hexokinase, and in the absence of glucose, causes a Crabtree effect.…”

Section: Resultsmentioning

confidence: 99%

“…cose readily enters renal tubular cells (20), undergoes phosphorylation by hexokinase, and then competitively inhibits further glycolysis of glucose-6-phosphate at the phosphoglucose isomerase enzyme (21). In the presence of 2-deoxyglucose and the absence of intraluminal Pi, Jv averaged 1.01±0.15 nl/mm min and the glucose absorptive flux averaged 34.0±3.5 pmol/mm min, which was not different from the base-line glucose transport rate (Table I).…”

Section: Resultsmentioning

confidence: 99%

Metabolic Requirement for Inorganic Phosphate by the Rabbit Proximal Tubule

Brazy¹,

Gullans²,

Mandel³

et al. 1982

J. Clin. Invest.

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A B S T R A C T These studies examine the effects of acute changes in the availability of inorganic phosphate on the function of isolated proximal renal tubules from rabbit kidney. We removed phosphate from the extracellular fluids and measured fluid absorption rates in isolated. perfused tubules and oxygen consumption rates in suspensions of cortical tubules. In proximal convoluted tubules, the selective removal of phosphate from the luminal fluid reduced fluid absorption rates from 1.11±0.12 to -0.01±0.08 nl/mmmin. This effect on fluid absorption was dependent on the presence of glucose transport and metabolism. The addition of phlorizin to the phosphate-free luminal fluid preserved fluid absorption rates (1.12±0.12 nl/ mm -min) as did the substitution of nonmetabolized a-methyl D-glucopyranoside for glucose (1.05±0.21 nl/mm min) or the addition of 2-deoxyglucose, an inhibitor of glycolysis, to the bathing medium (1.01±0.15 nl/mm -min). There was no effect on fluid absorption if phosphate was removed from the bath only. Additionally, removal of phosphate from the luminal fluid of proximal straight rather than convoluted tubules had no effect on fluid absorption rates. Oxygen consumption rates in suspensions of cortical tubules were reduced from 18.9±0.6 to 10.6±0.6 nmol O2/mg tubular protein * min by the removal of phosphate from the medium. This inhibition was prevented by the substitution of a-methyl D-glucopyranoside for glucose in the phosphate-free medium. The data indicate that under certain conditions, proximal convoluted tubules require the presence of phosphate in the luminal fluid to preserve tubular function. In the absence of intraluminal phosphate, glucose metabolism causes a reduction in both oxidative metab-

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“…In the small intestines 3-OMeG and glucose are actively transported by a common transport system but 2-dOG is not a substrate for this system (27,28, see also ref. 29). In erythrocytes, however, which transport sugars by facilitated diffusion, 3-OMeG, glucose, and 2-dOG use the same transport system (30).…”

Section: Competition Experimentsmentioning

confidence: 99%

Sugar Transport in Normal and Rous Sarcoma Virus-Transformed Chick-Embryo Fibroblasts

Venuta

Rubin

1973

Proc. Natl. Acad. Sci. U.S.A.

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ABSTRACT3-O-methylglucose (3-OMeG) is a nonmetabolizable glucose analog and is, therefore, suitable for transport studies. 3-OMeG and glucose compete for entry into normal and Rous sarcoma virus (RSV)-transformed chick-embryo fibroblasts. Therefore, 3-OMeG can be used to study the transport of glucose in these cells. Chickembryo fibroblasts infected and transformed by RSV take up 3-OMeG at a faster rate than uninfected cells when both cell types are growing at the same rate. The rate of efflux of 3-OMeG also increases after transformation. When the uptake and the efflux reach a steady state, the intracellular concentration of 3-OMeG is equal to the concentration in the medium. This finding indicates that glucose is transported across the plasma membrane by facilitated diffusion. The V. of the transport system for 3-OMeG increases after transformation, while the affinity or Km of the system remains unchanged. We conclude that viral transformation causes a change in the plasma membrane of the infected cells by increasing either the number of molecules or the mobility of the glucose carrier.Avian or mammalian cells can be transformed in vitro by RNA tumor viruses. The transformed cells are characterized by altered morphology, random orientation, and release from density-dependent inhibition (1). One of the earliest manifestations of the transformation is an increased rate of glucose accumulation inside the cells (2, 3).Temperature-sensitive mutants of Rous sarcoma virus (RSV) have been isolated (3-5). These mutants transform chick-embryo fibroblasts (CK) only at low temperature (36°), while they can infect and replicate at both high (410) and low temperatures. Thus, it is possible to distinguish between early and late events of the infection-transformation process because transformation can be initiated synchronously in the whole cell population simply by shifting the temperature of cells infected at 41°-36°.We studied (2) the rate of 2-deoxy-D-glucose (2-dOG) accumulation inside chick-embryo fibroblasts infected with one of the mutants described above, T5 (4). 2-deoxy-D-glucose is a glucose analog that is taken up and phosphorylated by the cell, but is not further metabolized (6, 7). 2-dOG and glucose compete with one another in normal and RSV-transformed chick-embryo fibroblasts, CK(RSV) (Venuta, unpublished).

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Transport of monosaccharides in kidney-cortex cells

Cited by 94 publications

References 16 publications

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Metabolic Requirement for Inorganic Phosphate by the Rabbit Proximal Tubule

Sugar Transport in Normal and Rous Sarcoma Virus-Transformed Chick-Embryo Fibroblasts

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Transport of monosaccharides in kidney-cortex cells

Cited by 94 publications

References 16 publications

Effects of Na+ and K+ on the uptake of metaraminol by rabbit ventricular slices

Effects of Na+ and K+ on the uptake of metaraminol by rabbit ventricular slices

Metabolic Requirement for Inorganic Phosphate by the Rabbit Proximal Tubule

Sugar Transport in Normal and Rous Sarcoma Virus-Transformed Chick-Embryo Fibroblasts

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Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices

Effects of Na⁺ and K⁺ on the uptake of metaraminol by rabbit ventricular slices