Transport of nucleic acid bases and nucleosides across the bacterial membrane

Pandey, Narendra Kumar

doi:10.1111/j.1574-6968.1984.tb00177.x

Cited by 8 publications

(1 citation statement)

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“…The group translocation hypothesis used to explain nucleic acid bases transport was prevalent in the 1970s (Rader & Hochstadt, 1976), and this hypothesis states that the essential salvage pathway enzymes such as phosphoribosyltransferase are situated in the plasma membrane and facilitate the transport of nucleotide bases (Hochstadt, 1978). As the group translocation hypothesis has since been excluded by accumulated evidence (Pandey, 1984), purine nucleoside phosphorylases have been shown to be associated with the internal surface of the plasma membrane, whereas phosphoribosyltransferases appear to be located in the cytoplasm (Page & Burton, 1978). These early studies and hypotheses are inspiring, but were limited by the lack of visualization techniques.…”

Section: Introductionmentioning

confidence: 99%

Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme

Lin

Zhang

Zhu

et al. 2011

FEMS Microbiol Lett

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N-deoxyribosyltransferases are essential enzymes in the nucleotide salvage pathway of lactobacilli. They catalyze the exchange between the purine or pyrimidine bases of 2'-deoxyribonucleosides and free pyrimidine or purine bases. In general, N-deoxyribosyltransferases are referred to as cytoplasmic enzymes, although there is no experimental evidence for this subcellular localization. In this work, the subcellular localization of N-deoxyribosyltransferase II (NTD) from Lactobacillus fermentum was examined by subcellular fractionation, transmission electron microscopy, and fluorescence microscopy. Our results indicate that L. fermentum NTD are distributed not only in the cytoplasm but also on the cell wall surface, and further studies showed that surface-attached NTD can be released into the culture broth and conventional buffers.

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Section: Introductionmentioning

confidence: 99%

Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme

Lin

Zhang

Zhu

et al. 2011

FEMS Microbiol Lett

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DNA transfer through cell membranes in bacteria

Sabelnikov¹

1989

Journal of Electroanalytical Chemistry and Interfacial Electroc

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Nucleic acid transfer through cells membranes: Towards the underlying mechanisms

Sabelnikov

1994

Progress in Biophysics and Molecular Biology

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Various cases of DNA (RNA) transfer through membranes of living cells are reviewed. They are classified into two major categories: those which occur in Nature (natural transfer) and those imposed by various physical and chemical treatments of cells (induced transfer). Among the examples of natural transfer surveyed are the transfer during bacterial conjugation, genetic transformation, viral infection of bacteria, and nuclear membrane trafficking. Consideration of the induced transfer is focused on the two methods most widely used at present to introduce foreign genetic information into pro- and eukaryotic cells: Ca2+ (and some other divalent cations)-induced and calcium phosphate-induced transfer, and transfer during electroporation of cells. Emphasis is made on the underlying mechanisms of transfer, or rather on what is currently known about them. Energetic aspects of transfer are also discussed and different tentative models of transfer are presented.

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Transport of nucleic acid bases and nucleosides across the bacterial membrane

Cited by 8 publications

References 25 publications

Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme

Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme

DNA transfer through cell membranes in bacteria

Nucleic acid transfer through cells membranes: Towards the underlying mechanisms

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