At all times, prior to slaughter, pigs may experience stress from a range of handling practices, such as fasting, loading and transport, mixing, and interaction with humans. These factors can affect the welfare of pigs and carcass and meat quality, both individually and collectively. Preslaughter stress is both an animal welfare and a meat quality issue. Behavioral and physiological studies have revealed that poor handling practices at the farm, during transport and at the slaughter plant, have an adverse effect on pigs and may result in the loss of profits due to animal losses during transport and in lairage. Also, poor preslaughter handling can also lead to losses in carcass value as a result of reduced yield, the presence of lesions and bacterial contamination, and meat quality defects (e.g., pale, soft, exudative and dark, firm, dry pork). These economic losses can be limited by improving the design of facilities, controlling the environmental conditions, and implementing training programs for the correct animal handling at any stage preslaughter. The objective of this review was to review research findings on the effects of preslaughter practices on ante-mortem behavioral and physiological response in pigs, including muscle metabolism, and to provide recommendations aimed at limiting the impact of preslaughter handling on animal losses and pork quality.