Transport of <sup>13</sup>C-oleate in adipocytes measured using multi imaging mass spectrometry

Kleinfeld, Alan M.; Kampf, J. Patrick; Lechène, C.

doi:10.1016/j.jasms.2004.07.010

Cited by 68 publications

(83 citation statements)

References 37 publications

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“…Multiple laboratories have now shown that LCFAs enter cells by both a facilitated, regulatable, proteinmediated transport process and diffusion. [3][4][5][28][29][30][31][32][33][34][35][36][37][38][39] At the unbound LCFA concentrations that commonly exist in most mammals, X90% of LCFA uptake into key tissues occurs by Correlation between body weight and total epididymal fat pad weights over time in fat-fed OM and S5B/Pl rats in protocol 3. The illustrated correlation with a third-order polynomial has a correlation coefficient of r ¼ 0.9790 (Po0.001).…”

Section: Discussionmentioning

confidence: 99%

“…(3) Weights of both strains increased significantly with time (b 3 for S), but (4) the rate of increase from week to week was maximal during week 1 and thereafter slowed down with time (b 4 for S). (5) The fatfed rats of both strains gained weight faster than the chow (b 5 for the HFD excess), but (6) their weekly rate of increase diminished faster (b 6 for the HFD excess fall-off). A more detailed explanation of the approach will be found in Brown and Prescott.…”

Section: Protocol 2: Time Course Studymentioning

confidence: 99%

“…Uptake occurs via two discrete processes: regulatable, protein-mediated, facilitated transport and passive flip-flop (diffusion) across the plasma membrane. [3][4][5] We have demonstrated several fold upregulation of saturable LCFA uptake in adipocytes from single-gene obesity models such as the Zucker rat, 6 ob, db, fat and tubby mouse, 7 and in dietary models of obesity in both rats and mice. 7 A similar upregulation of LCFA uptake occurs in omental adipocytes from obese patients undergoing bariatric surgery.…”

Section: Introductionmentioning

confidence: 99%

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Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets

et al. 2008

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Objective: To determine whether strain differences in adipocyte uptake of long chain fatty acids (LCFAs) contribute to differences in weight gain by Osborne-Mendel (OM) and S5B/Pl rats (S) fed a high-fat diet (HFD). Subjects: Ninety-four adult (12-14-week old) male OM and S rats. Measurements: Body weight; epididymal fat pad weight; adipocyte size, number, LCFA uptake kinetics; and plasma insulin and leptin during administration of HFD or chow diets (CDs). Results: In both strains, rate of weight gain (RWG) was greater on an HFD than a CD; RWG on an HFD was greater, overall, in OM than S. A significant RWG increase occurred on days 1 and 2 in both strains. It was normalized in S by days 6-9 but persisted at least till day 14 in OM. RWGs were significantly correlated (Po0.001) with the V max for saturable adipocyte LCFA uptake (V max ). In S, an increase in V max on day 1 returned to baseline by day 7 and was correlated with both plasma insulin and leptin levels throughout. In OM, a greater increase in V max was evident by day 2, and persisted for at least 14 days, during which both insulin and leptin levels remained elevated. Growth in epididymal fat pads on the HFD correlated with body weight, reflecting hypertrophy in OM and both hypertrophy and hyperplasia in S. Conclusions: (a) Changes in V max contribute significantly to changes in RWG on HFDs. (b) There are important strain differences in circulating insulin and leptin responses to an HFD. (c) Both insulin and leptin responses to an HFD are closely correlated with V max of adipocyte fatty acid uptake in S animals, but suggest early onset of insulin resistance in OM. Thus, differences in hormonal regulation of adipocyte LCFA uptake may underlie the different responses of OM and S to HFD.

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Section: Discussionmentioning

confidence: 99%

Section: Protocol 2: Time Course Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets

et al. 2008

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“…1 Numerous analytical techniques, including electrochemical detection, 2-6 separation-based methods, 7-9 fluorescence microscopy, 10-13 and mass spectrometry [14][15][16][17][18][19][20][21][22][23][24] have been used to surmount the inherent difficulties of measurements down to the μm-, pL-, and zmole-scale to study the intricate chemistry that exists within single cells.…”

Section: Introductionmentioning

confidence: 99%

Secondary Ion MS Imaging To Relatively Quantify Cholesterol in the Membranes of Individual Cells from Differentially Treated Populations

et al. 2007

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Time of flight secondary ion mass spectrometry (ToF-SIMS) is a well-established bioanalytical method for directly imaging the chemical distribution across single-cells. Here we report a protocol for the use of SIMS imaging to comparatively quantify the relative difference in lipid composition between the plasma membranes of two cells. This development enables direct comparison of the chemical effects of different drug treatments and incubation conditions in the plasma membrane at the single-cell level. Relative, quantitative ToF-SIMS imaging has been used here to compare macrophage cells treated to contain elevated levels of cholesterol with respect to control cells. Insitu fluorescence microscopy with two different membrane dyes was used to discriminate morphologically similar but differentially treated cells prior to SIMS analysis. SIMS images of fluorescently identified cells reveal that the two populations of cells have distinct outer leaflet membrane compositions with the membranes of the cholesterol-treated macrophages containing more than twice the amount of cholesterol of control macrophages. Relative quantification with SIMS to compare the chemical composition of single-cells can provide valuable information about normal biological functions, causative agents of diseases, and possible therapies for diseases.

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“…We have synthesized 13 C and 15 N labeled polyglycine samples with the distance between the two labels and the local atomic environment of the 13 C label systematically varied. We have measured four masses in parallel: 12 C, 13 C, and two of 12 e are developing the methodology of multiisotope imaging mass spectrometry (MIMS), which combines a new generation of SIMS instrument, labeling with stable isotopes and quantitative image analysis software [1][2][3][4]. We use this methodology to measure molecular turnover in subcellular compartments using stable isotopes, in particular 15 N, as tracers.…”

mentioning

confidence: 99%

CN⁻ secondary ions form by recombination as demonstrated using multi-isotope mass spectrometry of ¹³C- and ¹⁵N-labeled polyglycine

McMahon

Saint-Cyr

Lechène

et al. 2006

J. Am. Soc. Mass Spectrom.

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Transport of ¹³C-oleate in adipocytes measured using multi imaging mass spectrometry

Cited by 68 publications

References 37 publications

Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets

Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets

Secondary Ion MS Imaging To Relatively Quantify Cholesterol in the Membranes of Individual Cells from Differentially Treated Populations

CN⁻ secondary ions form by recombination as demonstrated using multi-isotope mass spectrometry of ¹³C- and ¹⁵N-labeled polyglycine

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Transport of 13C-oleate in adipocytes measured using multi imaging mass spectrometry

Cited by 68 publications

References 37 publications

Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets

Differences in adipocyte long chain fatty acid uptake in Osborne–Mendel and S5B/Pl rats in response to high-fat diets

Secondary Ion MS Imaging To Relatively Quantify Cholesterol in the Membranes of Individual Cells from Differentially Treated Populations

CN− secondary ions form by recombination as demonstrated using multi-isotope mass spectrometry of 13C- and 15N-labeled polyglycine

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Transport of ¹³C-oleate in adipocytes measured using multi imaging mass spectrometry

CN⁻ secondary ions form by recombination as demonstrated using multi-isotope mass spectrometry of ¹³C- and ¹⁵N-labeled polyglycine