Transport Systems in Pseudomonas

Hoshino, T

doi:10.1007/978-1-4899-0120-0_6

Cited by 3 publications

(3 citation statements)

References 116 publications

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“…In addition, we showed that the expression of the crcZ promoter responds similarly to both P. aeruginosa and P. putida CbrA () even if slight differences in the overall hierarchy of the utilization of carbon sources could be observed. These variations in crcZ expression in the different media could reflect some differences in the carbon source uptake efficiency between the two species (Hoshino, 1998). Moreover, we also observed that crcZ expression in P. putida is modulated depending on the carbon source used, similarly as in P. aeruginosa , emphasizing the fact that the TCSs of both Pseudomonas species work similarly and that both TCSs respond to the same signals ().…”

Section: Discussionmentioning

confidence: 99%

Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida

et al. 2014

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The CbrA/B system in pseudomonads is involved in the utilization of carbon sources and carbon catabolite repression (CCR) through the activation of the small RNAs crcZ in Pseudomonas aeruginosa, and crcZ and crcY in Pseudomonas putida. Interestingly, previous works reported that the CbrA/B system activity in P. aeruginosa PAO1 and P. putida KT2442 responded differently to the presence of different carbon sources, thus raising the question of the exact nature of the signal(s) detected by CbrA. Here, we demonstrated that the CbrA/B/CrcZ(Y) signal transduction pathway is similarly activated in the two Pseudomonas species. We show that the CbrA sensor kinase is fully interchangeable between the two species and, moreover, responds similarly to the presence of different carbon sources. In addition, a metabolomics analysis supported the hypothesis that CCR responds to the internal energy status of the cell, as the internal carbon/nitrogen ratio seems to determine CCR and non-CCR conditions. The strong difference found in the 2-oxoglutarate/glutamine ratio between CCR and non-CCR conditions points to the close relationship between carbon and nitrogen availability, or the relationship between the CbrA/B and NtrB/C systems, suggesting that both regulatory systems sense the same sort or interrelated signal.

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Section: Discussionmentioning

confidence: 99%

Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida

et al. 2014

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“…coli , the low‐affinity Pit transporters assimilate P i under P i ‐replete conditions and the high‐affinity Pst system is induced under P i ‐depleting conditions (Hoffer et al ., 2001a; Hsieh and Wanner, 2010; McCleary, 2017). Similar trends of transitioning to the Pst system with reduced P i availability were observed in other soil bacteria such as Bacillus species, Burkholderia cenocepacia , Pseudomonas aeruginosa , Sinorhizobium meliloti , Streptomyces coelicolor and Staphylococcus aureus (Qi et al ., 1997; Voegele et al ., 1997; Hoshino, 1998; Antelmann et al ., 2000; Saier Jr et al ., 2002; Hoi et al ., 2006; Voigt et al ., 2006; Martín et al ., 2011; Kelliher et al ., 2018; Barreiro and Martínez‐Castro, 2019; Shropshire et al ., 2021). Though P i is the most favourable form of P to bacteria, soil bacteria are also capable of assimilating P o compounds such as organophosphates and phosphonates (Table 1).…”

Section: Acquisition Of Pi and Po Compounds By Soil Bacteriamentioning

confidence: 99%

Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation

Park

Solhtalab

Thongsomboon

et al. 2022

Environ Microbiol Rep

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Summary Critical to meeting cellular phosphorus (P) demand, soil bacteria deploy a number of strategies to overcome limitation in inorganic P (Pi) in soils. As a significant contributor to P recycling, soil bacteria secrete extracellular enzymes to degrade organic P (Po) in soils into the readily bioavailable Pi. In addition, several Po compounds can be transported directly via specific transporters and subsequently enter intracellular metabolic pathways. In this review, we highlight the strategies that soil bacteria employ to recycle Po from the soil environment. We discuss the diversity of extracellular phosphatases in soils, the selectivity of these enzymes towards various Po biomolecules and the influence of the soil environmental conditions on the enzyme's activities. Moreover, we outline the intracellular metabolic pathways for Po biosynthesis and transporter‐assisted Po and Pi uptake at different Pi availabilities. We further highlight the regulatory mechanisms that govern the production of phosphatases, the expression of Po transporters and the key metabolic changes in P metabolism in response to environmental Pi availability. Due to the depletion of natural resources for Pi, we propose future studies needed to leverage bacteria‐mediated P recycling from the large pools of Po in soils or organic wastes to benefit agricultural productivity.

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“…The PTS is the sole route of fructose entry into P. aeruginosa (29). Mutants in the PTS pathway failed to take up fructose but were able to grow on mannitol (which is converted to fructose before being metabolized).…”

Section: Phosphotransferase Systemmentioning

confidence: 99%

On the mechanism of solute uptake in Pseudomonas

Hancock¹

2003

Front Biosci

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Pseudomonas species have over 300 known and putative nutrient uptake systems enabling them to metabolize a large number of organic compounds, and thus inhabit many diverse ecological niches. The outer membrane of these organisms acts as a semi-permeable barrier, excluding many classes of potentially toxic molecules from the cell. Nutrients use specialized water-filled channels called porins to traverse this barrier. Entry into the cell is mediated by one of four classes of cytoplasmic membrane transporters: glycerol facilitators, phosphotransferase systems, primary active transporters, and secondary active transporters. The class of transporter used is dependent on the environmental conditions, as well as the type and concentration of solute. The recent advances in elucidating the structures and functional mechanisms of these uptake systems will be discussed in this review.

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Transport Systems in Pseudomonas

Cited by 3 publications

References 116 publications

Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida

Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida

Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation

On the mechanism of solute uptake in Pseudomonas

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