Transportable hyperpolarized metabolites

Abstract

Nuclear spin hyperpolarization of 13C-labelled metabolites by dissolution dynamic nuclear polarization can enhance the NMR signals of metabolites by several orders of magnitude, which has enabled in vivo metabolic imaging by MRI. However, because of the short lifetime of the hyperpolarized magnetization (typically <1 min), the polarization process must be carried out close to the point of use. Here we introduce a concept that markedly extends hyperpolarization lifetimes and enables the transportation of hyperpolarized metabolites. The hyperpolarized sample can thus be removed from the polarizer and stored or transported for use at remote MRI or NMR sites. We show that hyperpolarization in alanine and glycine survives 16 h storage and transport, maintaining overall polarization enhancements of up to three orders of magnitude.

Reifung von Proben beeinflusst die Effizienz der Kernpolarisation

“…Dies ist wahrscheinlich eine Folge von NPS,wie es von Ji et al [14] anhand von heterogenen Proben gezeigt wurde.D iese enthielten sowohl PA -arme Phasen, die langsame Aufbauprozesse aufwiesen, als auch PA -reiche Phasen, die schnellere Prozesse aufwiesen. Dies ist wahrscheinlich eine Folge von NPS,wie es von Ji et al [14] anhand von heterogenen Proben gezeigt wurde.D iese enthielten sowohl PA -arme Phasen, die langsame Aufbauprozesse aufwiesen, als auch PA -reiche Phasen, die schnellere Prozesse aufwiesen.…”

Multiple Quantum Coherences Hyperpolarized at Ultra‐Low Fields

“…During the past decades, various hyperpolarization techniques have been developed [1][2][3][4][5][6][7][8][9][10][11][12][13][14] to boost the magnetic resonance (MR) signal in order to bring new applications to industry [15][16][17] and life-sciences. During the past decades, various hyperpolarization techniques have been developed [1][2][3][4][5][6][7][8][9][10][11][12][13][14] to boost the magnetic resonance (MR) signal in order to bring new applications to industry [15][16][17] and life-sciences.…”

Preservation of Nuclear Spin Order by Precipitation

“…[13] Onea pproach employs heterogeneousm aterials, in which the hyperpolarized spin order is transportedb ys pin diffusion to regions distant from the unpaired electron sites. [4] Another approach is to induce radical formation at low temperature by ultraviolet irradiation. The UV-induced radicals are quenched when the sample is warmed.…”

“…[1][2][3][4][5][6][7][8][9][10] Clinical studies involving hyperpolarized [1-13 C]pyruvate have been used to monitor tumour metabolism. [2] In vivo imaging studies have also been performed using hyperpolarized [1-13 C]fumarate.…”

“…[1][2][3][4][5][6][7][8][9][10] Clinical studies involving hyperpolarized [1-13 C]pyruvate have been used to monitor tumour metabolism. [2] In vivo imaging studies have also been performed using hyperpolarized [1][2][3][4][5][6][7][8][9][10][11][12][13] C]fumarate. [3] Hyperpolarization methods are limited by an intrinsic longitudinalr elaxation time (T 1 )t hat governst he return of nonequilibriumm agnetization to thermal equilibrium.T he time for extractingt he sample from the hyperpolarizatione quipment, purification, transport, delivery,a nd signal acquisition is often similar to the nuclear T 1 ,m eaning that the polarizer needs to be located close to the point of use.…”