Transporter engineering for the development of cyanobacteria as cell factories: A text analytics guided survey

Sengupta, Shinjinee; Sahasrabuddhe, Deepti; Wangikar, Pramod P.

doi:10.1016/j.biotechadv.2021.107816

Cited by 7 publications

(5 citation statements)

References 140 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The above results confirmed the significant effects of inactivating fructokinase to unleash the potential of fructose synthesis in Syn7942, and they also refreshed our understanding of the flexibility of the cyanobacterial sugar metabolism and transport. Previously, the importance of transporter engineering has been widely emphasized in cyanobacterial metabolic engineering to facilitate the secretion and uptake of sugar metabolites. ,– The secretion of the target metabolites could relieve the intracellular metabolic stress, prevent potential feedback inhibition, and increase the final titer and productivities, and it would also fit the technological requirements of in situ product separation from the culture broth . Thus, Niederholtmeyer et al introduced a hexose transporter Glf into Syn7942 and achieved the secretion of both fructose and glucose, while in this study, we found that the blocking of fructokinase activities would stimulate the natural exporting capacities of Syn7942 for intracellular fructose, and over 97% of the synthesized fructose was secreted into the culture broth, which would decrease the costs and difficulties for product recovery in the subsequent routine.…”

Section: Resultsmentioning

confidence: 51%

“…16,23−25 The secretion of the target metabolites could relieve the intracellular metabolic stress, prevent potential feedback inhibition, and increase the final titer and productivities, and it would also fit the technological requirements of in situ product separation from the culture broth. 26 Thus, Niederholtmeyer et al introduced a hexose transporter Glf into Syn7942 and achieved the secretion of both fructose and glucose, 20 while in this study, we found that the blocking of fructokinase activities would stimulate the natural exporting capacities of Syn7942 for intracellular fructose, and over 97% of the synthesized fructose was secreted into the culture broth, which would decrease the costs and difficulties for product recovery in the subsequent routine. Although the exact transport mechanisms of the release of fructose from Syn7942 remain to be identified, it may be related to the following two explanations.…”

Section: ■ Results and Discussionmentioning

confidence: 54%

See 1 more Smart Citation

Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose

Sun,

Zhang,

Zhang

et al. 2023

ACS Synth. Biol.

View full text Add to dashboard Cite

Fructose is an important monosaccharide product widely applied in the food, medicine, and chemical industries. Currently, fructose is mainly manufactured with plant biomass-sourced polysaccharides through multiple steps of digestion, conversion, separation, and purification. The development of cyanobacterial metabolic engineering provides an attractive alternative route for the one-step direct production of fructose utilizing carbon dioxide and solar energy. In this work, we developed a paradigm for engineering cyanobacterial chassis cells into efficient cell factories for the photosynthetic production of fructose. In a representative cyanobacterial strain, Synechococcus elongatus PCC 7942, knockout of fructokinase effectively activated the synthesis and secretion of fructose in hypersaline conditions, independent of any heterologous transporters. The native sucrose synthesis pathway was identified as playing a primary role in fructose synthesis. Through combinatory optimizations on the levels of metabolism, physiology, and cultivation, the fructose yield of the Synechococcus cell factories was stepwise improved to 3.9 g/L. Such a paradigm was also adopted to engineer another Synechococcus strain, the marine species Synechococcus sp. PCC 7002, and facilitated an even higher fructose yield of over 6 g/L. Finally, the fructose synthesized and secreted by the cyanobacterial photosynthetic cell factories was successfully extracted and prepared from the culture broth in the form of products with 86% purity through multistep separation−purification operations. This work demonstrated a paradigm for systematically engineering cyanobacteria for photosynthetic production of desired metabolites, and it also confirmed the feasibility and potential of cyanobacterial photosynthetic biomanufacturing as a simple and efficient route for fructose production.

show abstract

Section: Resultsmentioning

confidence: 51%

Section: ■ Results and Discussionmentioning

confidence: 54%

Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose

Sun,

Zhang,

Zhang

et al. 2023

ACS Synth. Biol.

View full text Add to dashboard Cite

show abstract

“… 70 , 71 Glucose transporters thus facilitate the transport of glucose into cells while glycolytic enzymes catalyze the breakdown of glucose into energy. 69 – 72 Moreover, mTORC1 has been found to enhance the translation of the HIF-1α which in turn activates the expression of key enzymes involved in glycolysis like phospho-fructokinase. The mTORC1-mediated SREBP stimulation caused increased flux by the pentose phosphate pathway leading to the generation of NADPH from the glucose and other intermediate metabolites for proliferation and growth.…”

Section: Upstream Regulators Of Mtor Signalingmentioning

confidence: 99%

Multifaceted role of mTOR (mammalian target of rapamycin) signaling pathway in human health and disease

Panwar,

Singh,

Bhatt

et al. 2023

Sig Transduct Target Ther

Self Cite

190

View full text Add to dashboard Cite

The mammalian target of rapamycin (mTOR) is a protein kinase that controls cellular metabolism, catabolism, immune responses, autophagy, survival, proliferation, and migration, to maintain cellular homeostasis. The mTOR signaling cascade consists of two distinct multi-subunit complexes named mTOR complex 1/2 (mTORC1/2). mTOR catalyzes the phosphorylation of several critical proteins like AKT, protein kinase C, insulin growth factor receptor (IGF-1R), 4E binding protein 1 (4E-BP1), ribosomal protein S6 kinase (S6K), transcription factor EB (TFEB), sterol-responsive element-binding proteins (SREBPs), Lipin-1, and Unc-51-like autophagy-activating kinases. mTOR signaling plays a central role in regulating translation, lipid synthesis, nucleotide synthesis, biogenesis of lysosomes, nutrient sensing, and growth factor signaling. The emerging pieces of evidence have revealed that the constitutive activation of the mTOR pathway due to mutations/amplification/deletion in either mTOR and its complexes (mTORC1 and mTORC2) or upstream targets is responsible for aging, neurological diseases, and human malignancies. Here, we provide the detailed structure of mTOR, its complexes, and the comprehensive role of upstream regulators, as well as downstream effectors of mTOR signaling cascades in the metabolism, biogenesis of biomolecules, immune responses, and autophagy. Additionally, we summarize the potential of long noncoding RNAs (lncRNAs) as an important modulator of mTOR signaling. Importantly, we have highlighted the potential of mTOR signaling in aging, neurological disorders, human cancers, cancer stem cells, and drug resistance. Here, we discuss the developments for the therapeutic targeting of mTOR signaling with improved anticancer efficacy for the benefit of cancer patients in clinics.

show abstract

“…The combined regulatory strain GalP93-GIk37 significantly increased the rate of glucose consumption. In addition to the promoter library, the regulation of single genes of the succinic acid transporters DcuB and DcuC by RBS library can also improve the ability to transport succinic acid to the cell and increase the yield of the final product 2,3-butanediol [ 109 – 111 ]. Multi-gene regulation …”

Section: Technical Approaches For Overcoming Low Efficiency In Carbon...mentioning

confidence: 99%

Biosynthesis pathways of expanding carbon chains for producing advanced biofuels

Lin

2023

Biotechnol Biofuels

View full text Add to dashboard Cite

Because the thermodynamic property is closer to gasoline, advanced biofuels (C ≥ 6) are appealing for replacing non-renewable fossil fuels using biosynthesis method that has presented a promising approach. Synthesizing advanced biofuels (C ≥ 6), in general, requires the expansion of carbon chains from three carbon atoms to more than six carbon atoms. Despite some specific biosynthesis pathways that have been developed in recent years, adequate summary is still lacking on how to obtain an effective metabolic pathway. Review of biosynthesis pathways for expanding carbon chains will be conducive to selecting, optimizing and discovering novel synthetic route to obtain new advanced biofuels. Herein, we first highlighted challenges on expanding carbon chains, followed by presentation of two biosynthesis strategies and review of three different types of biosynthesis pathways of carbon chain expansion for synthesizing advanced biofuels. Finally, we provided an outlook for the introduction of gene-editing technology in the development of new biosynthesis pathways of carbon chain expansion.

show abstract

Transporter engineering for the development of cyanobacteria as cell factories: A text analytics guided survey

Cited by 7 publications

References 140 publications

Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose

Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose

Multifaceted role of mTOR (mammalian target of rapamycin) signaling pathway in human health and disease

Biosynthesis pathways of expanding carbon chains for producing advanced biofuels

Contact Info

Product

Resources

About