Transposable elements and genomics

Kim, Namsoo

doi:10.1007/s13258-014-0262-1

Cited by 2 publications

(3 citation statements)

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“…Research on plasmid-mediated homologous recombination showed some successful examples using intermolecular recombination between two circular molecules (Poustka et al, 1984;Philippe et al, 2004;2014b;2015). In this method, the host cell must have the genetic background of recA + , which is called RecA-dependent strategy.…”

Section: Plasmid-mediated Homologous Recombinationmentioning

confidence: 99%

“…Transposons are mobile genetic elements that can transpose from one location in a genome to another (Judson and Mekalanos, 2000). Transposon insertions can alter the regulation and expression of genes, so the application of it has provided valuable functional genomic information to (Kim, 2015). However, the drawbacks of this method are that it is difficult to identify the transposon insertion sites, and requires complex procedures (Suzuki et al, 2006).…”

Section: Gene Inactivation Based On Insertional Inactivationmentioning

confidence: 99%

“…(Hou et al, 2012;2014a;2014b;2015). Although the high-producing strains were obtained via repeated physical and/or chemical mutagenesis, there are many disadvantages to the processes, such as slow-growth, low sugar consumption rating, and low stress tolerance .…”

Section: Introductionmentioning

confidence: 99%

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Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression

Zhang

2016

J. Zhejiang Univ. Sci. B

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Abstract:With the availability of the whole genome sequence of Escherichia coli or Corynebacterium glutamicum, strategies for directed DNA manipulation have developed rapidly. DNA manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. DNA manipulation involves modifying the autologous genes and expressing the heterogenous genes. Two alternative approaches, using electroporation linear DNA or recombinant suicide plasmid, allow a wide variety of DNA manipulation. However, the over-expression of the desired gene is generally executed via plasmid-mediation. The current review summarizes the common strategies used for genetically modifying E. coli and C. glutamicum genomes, and discusses the technical problem of multi-layered DNA manipulation. Strategies for gene over-expression via integrating into genome are proposed. This review is intended to be an accessible introduction to DNA manipulation within the bacterial genome for novices and a source of the latest experimental information for experienced investigators.

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Section: Plasmid-mediated Homologous Recombinationmentioning

confidence: 99%