2021
DOI: 10.3791/62843
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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a <em>Burkholderia gladioli</em> Symbiont of <em>Lagria villosa</em> Beetles

Abstract: Inferring the function of genes by manipulating their activity is an essential tool for understanding the genetic underpinnings of most biological processes. Advances in molecular microbiology have seen the emergence of diverse mutagenesis techniques for the manipulation of genes. Among them, transposon-insertion sequencing (Tn-seq) is a valuable tool to simultaneously assess the functionality of many candidate genes in an untargeted way. The technique has been key to identify molecular mechanisms for the colo… Show more

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“…Alternatively, genome-wide manipulation (e.g. via transposon-insertion sequencing) can be leveraged to identify candidate genes (Ganesan et al 2021). Following identification, targeted manipulation of candidate genes and complementation of knock-out mutants allows for the determination of biochemical pathways underlying genetic basis of detoxification (Sato et al 2021).…”
Section: The Molecular/microbiological Routementioning
confidence: 99%
“…Alternatively, genome-wide manipulation (e.g. via transposon-insertion sequencing) can be leveraged to identify candidate genes (Ganesan et al 2021). Following identification, targeted manipulation of candidate genes and complementation of knock-out mutants allows for the determination of biochemical pathways underlying genetic basis of detoxification (Sato et al 2021).…”
Section: The Molecular/microbiological Routementioning
confidence: 99%