Transpulmonary cardiac output measurement in a rat model of cardiac arrest and CPR: Impact of vascular access

Kelm, Robert F.; Wagenführer, Jürgen; Schmidtmann, Irene; Engelhard, Kristin; Werner, Christian; Noppens, Rüdiger

doi:10.1016/j.resuscitation.2009.10.024

Cited by 4 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All animals were fasted overnight with free access to water before surgery. Experimental procedures on rats were performed as previously described (16). In brief, animals were anesthetized (induction, sevoflurane 4%; maintenance, sevoflurane 2.5 %), orally intubated, and ventilated (30%/70% inspiratory oxygen/air at all times); end-tidal carbon dioxide tension was controlled at values of 40 mm Hg using capnography.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*

Noppens

Kelm

Lindemann

et al. 2012

Critical Care Medicine

Self Cite

View full text Add to dashboard Cite

Despite promising results in other models of brain injury, hypertonic saline hydroxyethyl starch failed to improve the outcome when administered after asphyxic cardiac arrest/cardiopulmonary resuscitation in rats. One major difference between the cardiac arrest/cardiopulmonary resuscitation model and other models of brain ischemia is that the effects of asphyxic cardiac arrest involve the whole organism (post-cardiac arrest syndrome) and not exclusively the brain leading to a more severe injury. This might explain why hypertonic saline hydroxyethyl starch has failed to improve outcome in the present model.

show abstract

Section: Methodsmentioning

confidence: 99%

“…CA was defined as a MAP 20 mm Hg. Nine minutes after induction of apnea, CPR was initiated with external chest compressions (200 min −1 ) and controlled ventilation was started (16). A single dose of adrenaline was injected IV (10 µg/kg) via the right femoral vein catheter.…”

Section: Methodsmentioning

confidence: 99%

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*

Noppens

Kelm

Lindemann

et al. 2012

Critical Care Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Experimental procedures were performed as previously described (29). Briefly, anesthesia was induced with sevoflurane 4 vol% and an Fio 2 of 0.8.…”

Section: Animal Preparationmentioning

confidence: 99%

Effects of Levosimendan on Hemodynamics, Local Cerebral Blood Flow, Neuronal Injury, and Neuroinflammation After Asphyctic Cardiac Arrest in Rats

Kelm

Wagenführer

Bauer

et al. 2014

Critical Care Medicine

Self Cite

View full text Add to dashboard Cite

Levosimendan increased cerebral blood flow after experimental cardiac arrest/cardiopulmonary resuscitation. This effect coincided with reduced neuronal injury and improved neurologic outcome. Findings seem to be independent of inflammatory effects because no effects by levosimendan on cerebral or systemic inflammation could be detected. In summary, levosimendan is a promising agent to improve neurological outcome after cardiac arrest/cardiopulmonary resuscitation.

show abstract

“…The pre-DEM fraction is much larger than the 7.4% lung retention in vivo reported by Neirinckx et al (20). This difference is largely due to the large difference between the pump flow (10 mL/min) and the much higher resting cardiac output of an adult Sprague-Dawley rat (~100 mL/min) (15). In this case 99m Tc-HMPAO lung uptake predicted by the model using the parameters in Table 1 is~6%, providing additional validation of the model.…”

Section: Discussionmentioning

confidence: 90%

“…Simulations were performed to address questions pertinent to in vivo conditions that cannot be readily answered experimentally. A flow of 100 mL/min, corresponding to resting cardiac output of an adult male 350-g Sprague-Dawley rat (15), was used to determine the effect of the key model parameters on steady-state lung uptake. The baseline simulation was performed with model parameters in Table 2, which were rescaled from experiments performed with F ϭ 10 mL/min or obtained from previous studies (23).…”

Section: Pbpk Model Of 99m Tc-hmpao Pulmonary Dispositionmentioning

confidence: 99%

Pharmacokinetics of ^99mTc-HMPAO in isolated perfused rat lungs

Clough

Barry

Rizzo

et al. 2019

Journal of Applied Physiology

View full text Add to dashboard Cite

Lung uptake of technetium-labeled hexamethylpropyleneamine oxime (HMPAO) increases in rat models of human acute lung injury, consistent with increases in lung tissue glutathione (GSH). Since 99mTc-HMPAO uptake is the net result of multiple cellular and vascular processes, the objective was to develop an approach to investigate the pharmacokinetics of 99mTc-HMPAO uptake in isolated perfused rat lungs. Lungs of anesthetized rats were excised and connected to a ventilation-perfusion system. 99mTc-HMPAO (56 MBq) was injected into the pulmonary arterial cannula, a time sequence of images was acquired, and lung time-activity curves were constructed. Imaging was repeated with a range of pump flows and perfusate albumin concentrations and before and after depletion of GSH with diethyl maleate (DEM). A pharmacokinetic model of 99mTc-HMPAO pulmonary disposition was developed and used for quantitative interpretation of the time-activity curves. Experimental results reveal that 99mTc-HMPAO lung uptake, defined as the steady-state value of the 99mTc-HMPAO lung time-activity curve, was inversely related to pump flow. Also, 99mTc-HMPAO lung uptake decreased by ~65% after addition of DEM to the perfusate. Increased perfusate albumin concentration also resulted in decreased 99mTc-HMPAO lung uptake. Model simulations under in vivo flow conditions indicate that lung tissue GSH is the dominant factor in 99mTc-HMPAO retention in lung tissue. The approach allows for evaluation of the dominant factors that determine imaging biomarker uptake, separation of the contributions of pulmonary versus systemic processes, and application of this knowledge to in vivo studies. NEW & NOTEWORTHY We developed an approach for studying the pharmacokinetics of technetium-labeled hexamethylpropyleneamine oxime (99mTc-HMPAO) in isolated perfused lungs. A distributed-in-space-and-time computational model was fit to data and used to investigate questions that cannot readily be addressed in vivo. Experimental and modeling results indicate that tissue GSH is the dominant factor in 99mTc-HMPAO retention in lung tissue. This modeling approach can be readily extended to investigate the lung pharmacokinetics of other biomarkers and models of lung injury and treatment thereof.

show abstract

Transpulmonary cardiac output measurement in a rat model of cardiac arrest and CPR: Impact of vascular access

Cited by 4 publications

References 59 publications

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*

Effects of Levosimendan on Hemodynamics, Local Cerebral Blood Flow, Neuronal Injury, and Neuroinflammation After Asphyctic Cardiac Arrest in Rats

Pharmacokinetics of ^99mTc-HMPAO in isolated perfused rat lungs

Contact Info

Product

Resources

About

Transpulmonary cardiac output measurement in a rat model of cardiac arrest and CPR: Impact of vascular access

Cited by 4 publications

References 59 publications

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*

Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats*

Effects of Levosimendan on Hemodynamics, Local Cerebral Blood Flow, Neuronal Injury, and Neuroinflammation After Asphyctic Cardiac Arrest in Rats

Pharmacokinetics of 99mTc-HMPAO in isolated perfused rat lungs

Contact Info

Product

Resources

About

Pharmacokinetics of ^99mTc-HMPAO in isolated perfused rat lungs