Transvaginal, ultrasound-guided biopsy of the corpus luteum in cattle

Kot, Karolina; Anderson, L. E.; Tsai, Shaw-Jenq; Wiltbank, Milo C.; Ginther, O.J.

doi:10.1016/s0093-691x(99)00188-0

Cited by 21 publications

(11 citation statements)

References 14 publications

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“…Functionally, luteal production of progesterone decreases by 4 h after PGF 2α treatment and reaches basal concentrations by 48 h after PGF 2α treatment (for example see Rodgers et al (1995)). Transvaginal ultrasonography can be used to collect a small amount (20 µl) of follicular fluid (Ginther et al, 1997) or luteal tissue (Kot et al, 1999) without altering subsequent ovarian function. These studies involved either cell culture techniques or removal of corpora lutea at specific times after treatment to analyse PGF 2α action.…”

Section: Introductionmentioning

confidence: 99%

Temporal gene expression in bovine corpora lutea after treatment with PGF2alpha based on serial biopsies in vivo

Tsai¹,

Kot²,

Ginther³

et al. 2001

Reproduction

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There is growing evidence to indicate that PGF(2alpha)-induced luteolysis involves altered gene expression in the corpus luteum. Concentrations of mRNA encoding nine different gene products were quantified at three time points from corpora lutea in situ. Serial luteal biopsies (2.1-5.5 mg per biopsy) were collected using an ultrasound-guided transvaginal method and mRNA concentrations were quantified with standard curve quantitative competitive RT-PCR. In the first experiment, three luteal biopsies were collected from three heifers and analysed in multiple assays to evaluate the repeatability of the methods. Concentrations of mRNA for glyceraldehyde 3-phosphate dehydrogenase (GAPDH), PGF(2alpha) receptor (FP receptor) and LH receptor were found to be highly repeatable between assays, between multiple biopsies and between animals (coefficients of variation 1.3-17.3%). In the second experiment, heifers on days 9-11 after ovulation were assigned randomly to receive saline only (n = 6), saline with biopsies taken at t = 0, 0.5 and 4.0 h after injection (n = 6), PGF(2alpha) only (n = 6) or PGF(2alpha) with biopsies taken at t = 0, 0.5 and 4.0 h after treatment (n = 7). Biopsy alone did not change corpus luteum diameter, serum progesterone concentrations or days to next ovulation within the saline- or PGF(2alpha)-treated groups. Concentrations of mRNA for steroidogenic acute regulatory protein, FP receptor, 3beta-hydroxysteroid dehydrogenase, cytosolic phospholipase A(2) and LH receptor were decreased at 4.0 h after PGF(2alpha) injection. In contrast, PGF(2alpha) increased mRNA concentrations for prostaglandin G/H synthase-2, monocyte chemoattractant protein-1 and c-fos but the time course differed for induction of these mRNAs. Concentrations of mRNA for GAPDH did not change after PGF(2alpha) treatment. In conclusion, the techniques allowed analysis of multiple, specific mRNAs in an individual corpus luteum at multiple time points without altering subsequent luteal function. Use of these techniques confirmed that luteolysis involves both up- and downregulation of specific mRNA by PGF(2alpha).

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Section: Introductionmentioning

confidence: 99%

Temporal gene expression in bovine corpora lutea after treatment with PGF2alpha based on serial biopsies in vivo

Tsai¹,

Kot²,

Ginther³

et al. 2001

Reproduction

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“…By using the ultrasound technique we have the opportunity to observe the follicle and CL development without any influence on their physiology (Roelofs et al 2004). Moreover, the ultrasound technique offers additionally the possibility to collect target-oriented samples from follicles by ultrasound-guided follicle aspiration (Cech et al 2013) or from the CL by an ultrasound-guided biopsy (Kot et al 1999). In heifers, as well as in cows, it is described that following sample collection of follicle fluid by follicle aspiration from a preovulatory follicle (after the luteinising hormone surge), development of a CL does occur (Hayashi et al 2006;O'Hara et al 2012).…”

mentioning

confidence: 99%

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

Vernunft¹,

Weitzel²,

Viergutz³

2013

Vet. Med. - Czech

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Secretion of adequate levels of progesterone from a proper corpus luteum (CL), which develops out of the cells of a healthy preovulatory follicle, is a key-factor for establishment of a pregnancy. The aim of this study was to investigate the relationship between morphological and secretory characteristics of preovulatory follicles and their corresponding corpus luteum with regard to the post-partum period in high-yielding dairy cows. Therefore, ultrasound-guided aspirations of preovulatory follicles were performed repeatedly, using 20 first lactating cows between 26 and 121 days after parturition. Heat was induced with a PGF analogon followed by administration of a GnRH analogon. The dominant follicle was aspirated 21 h after administration of the GnRH analogon. The diameters of the follicles were estimated at aspiration and the morphology of the resulting luteal tissue was examined on day 14 after follicle aspiration using ultrasonographic examinations. Concentrations of progesterone (P 4 ) and 17-beta-oestradiol (E 2 ) were determined in the follicular fluids (FF) and P 4 concentration was estimated at the time of CL examination in plasma. A CL development occurred in 82% after dominant follicle aspiration. The interval of time between parturition and follicle aspiration did not affect the investigated variables. The diameter of the aspirated preovulatory follicle was positively correlated to the cross-section area of the developed luteal tissue (R = 0.60; P < 0.01) as well as to the plasma P 4 concentration on day 14 after follicle aspiration (R = 0.47; P < 0.05). Also, E 2 concentrations in FF were positively correlated to cross-section area of the luteal tissue (R = 0.54; P < 0.05). Comparing the FF of the follicles that gave rise to a CL after aspiration to follicles that did not, both types had comparable P 4 , but the former type harboured higher E 2 concentrations. In conclusion, preovulatory follicle diameter as well as steroid concentrations in the follicular fluid could be used prospectively to identify cows which will have well-developed CLs and high plasma P 4 levels later. On the other hand, CL development after follicle aspiration can be used as a retrospective quality parameter of dominant follicles. These results will help to identify suitable animals for breeding or recipients for embryo transfer.Keywords: post-partum period; ultrasound; fertility; progesterone; oestradiol; follicle fluid List of abbreviations CL = corpus luteum, DM = dry matter, E 2 = 17-beta-oestradiol, FF = follicle fluid, GnRH = gonadotropin realising hormone, P 4 = progesterone, PGF = prostaglandin F2 alfa, TMR = total mixed ration

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“…Other possible in vivo procedures with OTU include injections in individual follicles (Beg & Ginther 2006), ovarian stroma (Oropeza et al, 2004) and corpus luteum (CL) (Yamashita et al, 2008). Ovarian biopsies can also be performed with OTU for the collection of CL (Kot et al, 1999) and ovarian cortical samples (Aerts et al, 2005). Moreover, OTU allows the in vivo transfer of oocytes from one ovarian follicle to another (i.e.…”

Section: Methods For In Vivo Gene Delivery In the Reproductive Tract mentioning

confidence: 99%

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

Velazquez¹,

Kues²

2011

Biomedical Engineering, Trends, Research and Technologies

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Transvaginal, ultrasound-guided biopsy of the corpus luteum in cattle

Cited by 21 publications

References 14 publications

Temporal gene expression in bovine corpora lutea after treatment with PGF2alpha based on serial biopsies in vivo

Temporal gene expression in bovine corpora lutea after treatment with PGF2alpha based on serial biopsies in vivo

Corpus luteum development and its morphology after aspiration of a preovulatory follicle is related to size and steroid content of the follicle in dairy cows

In Vivo Gene Transfer in the Female Bovine: Potential Applications for Biomedical Research in Reproductive Sciences

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