Transverse and axial resolution of femtosecond laser ablation

Abstract

Femtosecond lasers are capable of precise ablation that produce surgical dissections in vivo. The transverse and axial resolution of the laser damage inside the bulk are important parameters of ablation. The transverse resolution is routinely quantified, but the axial resolution is more difficult to measure and is less commonly performed. In some in vivo samples, fine dissections can also be difficult to visualize, but in vitro samples may allow clear imaging. Using a 1040-nm, 400-fs pulsed laser, we performed ablation inside agarose and glass, producing clear and persistent damage spots. Near the ablation threshold of both media, we found that the axial resolution is similar to the transverse resolution. We also ablated neuron cell bodies and fibers in C. elegans and demonstrate submicrometer resolution in both the transverse and axial directions, consistent with our results in agarose and glass. Using simple yet rigorous methods, we define the resolution of laser ablation in transparent media along all directions.

Expression of thioredoxin-1 in the ASJ neuron corresponds with and enhances intrinsic regenerative capacity under lesion conditioning inC. elegans

“…We followed established procedures for animal immobilization by sodium azide (Chung, et al ., 2006) and femtosecond laser surgery (Harreguy, et al ., 2020; Wang, et al ., 2022). We severed only the axon (within 2 μm from the cell body), only the dendrite (2/3 rd the length distal from the cell body), or both the axon and dendrite (axon+dendrite), and we reimaged 48 hours afterwards.…”

“…We followed established procedures for animal immobilization by sodium azide (Chung, et al, 2006) and femtosecond laser surgery (Harreguy, et al, 2020;Wang, et al, 2022).…”