Transwell Insert-Embedded Microfluidic Devices for Time-Lapse Monitoring of Alveolar Epithelium Barrier Function under Various Stimulations

Chang, Shu-Han; Ko, Ping-Liang; Liao, Wei-Hao; Peng, Chien-Chung; Tung, Yi-Chung

doi:10.3390/mi12040406

Cited by 11 publications

(8 citation statements)

References 34 publications

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“…This cell line, known as called hALEVI, has been shown to form tight junctions and to be culturable for many passages in submerged and ALI conditions [185]. It also was used to study the influence of volatile ethanol on tight junctional structures [186].…”

Section: Cell Linesmentioning

confidence: 99%

“…Tumor derived [157] Yes [5,56,57,[158][159][160][161][162][163] Calu-3 Epithelial adenocarcinoma Tumor derived [166] Yes [165,167,168] BEAS-2B Bronchial epithelium SV40 T-antigen [170] Yes [113,171,172] 16HBE14o-Bronchial epithelium SV40 T-antigen [187] Yes [173][174][175][176] NuLi-1 Bronchial epithelium hTERT [188] Yes [181,189] HBEC3-KT Bronchial epithelium hTERT [190] No BCi-NS1.1 Large airways basal cell hTERT [182] No hAELVI Primary alveolar epithelim Lentivirus [185] Yes [186] hSABCi-NS1.1 Small airway basal cell hTERT [183] No…”

Section: A549 Epithelial Adenocarcinomamentioning

confidence: 99%

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In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends

Cerimi

Jäckel

Meyer

et al. 2022

JoF

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Microbial volatile organic compounds (mVOC) are metabolic products and by-products of bacteria and fungi. They play an important role in the biosphere: They are responsible for inter- and intra-species communication and can positively or negatively affect growth in plants. But they can also cause discomfort and disease symptoms in humans. Although a link between mVOCs and respiratory health symptoms in humans has been demonstrated by numerous studies, standardized test systems for evaluating the toxicity of mVOCs are currently not available. Also, mVOCs are not considered systematically at regulatory level. We therefore performed a literature survey of existing in vitro exposure systems and lung models in order to summarize the state-of-the-art and discuss their suitability for understanding the potential toxic effects of mVOCs on human health. We present a review of submerged cultivation, air-liquid-interface (ALI), spheroids and organoids as well as multi-organ approaches and compare their advantages and disadvantages. Furthermore, we discuss the limitations of mVOC fingerprinting. However, given the most recent developments in the field, we expect that there will soon be adequate models of the human respiratory tract and its response to mVOCs.

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Section: Cell Linesmentioning

confidence: 99%

Section: A549 Epithelial Adenocarcinomamentioning

confidence: 99%

In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends

Cerimi

Jäckel

Meyer

et al. 2022

JoF

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show abstract

“…They demonstrated that such a device allowed the analysis of the effect of environmental agents on the airway epithelium with higher sensitivity compared to static models . More recently, the group of Tung demonstrated a device compatible with Transwell inserts and capable of vapor exposure and time-lapse monitoring of alveolar epithelium barrier function . However, both research’s groups did not explore the possibility to use the device for replicating airway disease, such as CF.…”

Section: Introductionmentioning

confidence: 99%

Easy-to-Build and Reusable Microfluidic Device for the Dynamic Culture of Human Bronchial Cystic Fibrosis Epithelia

Mazio

Scognamiglio

Passariello

et al. 2023

ACS Biomater. Sci. Eng.

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Cystic fibrosis (CF) is one of the most frequent genetic diseases, caused by dysfunction of the CF transmembrane conductance regulator (CFTR) chloride channel. CF particularly affects the epithelium of the respiratory system. Therapies aim at rescuing CFTR defects in the epithelium, but CF genetic heterogeneity hinders the finding of a single and generally effective treatment. Therefore, in vitro models have been developed to study CF and guide patient therapy. Here, we show a CF model on-chip by coupling the feasibility of the human bronchial epithelium differentiated in vitro at the air−liquid interface and the innovation of microfluidics. We demonstrate that the dynamic flow enhanced cilia distribution and increased mucus quantity, thus promoting tissue differentiation in a short time. The microfluidic devices highlighted differences between CF and non-CF epithelia, as shown by electrophysiological measures, mucus quantity, viscosity, and the analysis of ciliary beat frequency. The described model on-chip may be a handy instrument for studying CF and setting up therapies. As a proof of principle, we administrated the corrector VX-809 on-chip and observed a decrease in mucus thickness and viscosity.

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“…[ 8,11,22 ] To address the lack of flow capabilities in conventional open‐well assays, microfluidic platforms (e.g., microphysiological systems) that incorporate a porous culture membrane between the individually addressable top (luminal) and bottom (abluminal) fluidic channels have emerged. [ 23–40 ]…”

Section: Introductionmentioning

confidence: 99%

The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow

Mansouri

Ahmed

Ahmad

et al. 2022

Adv Healthcare Materials

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Microfluidic tissue barrier models have emerged to address the lack of physiological fluid flow in conventional "open-well" Transwell-like devices. However, microfluidic techniques have not achieved widespread usage in bioscience laboratories because they are not fully compatible with traditional experimental protocols. To advance barrier tissue research, there is a need for a platform that combines the key advantages of both conventional open-well and microfluidic systems. Here, a plug-and-play flow module is developed to introduce on-demand microfluidic flow capabilities to an open-well device that features a nanoporous membrane and live-cell imaging capabilities. The magnetic latching assembly of this design enables bi-directional reconfiguration and allows users to conduct an experiment in an open-well format with established protocols and then add or remove microfluidic capabilities as desired. This work also provides an experimentally-validated flow model to select flow conditions based on the experimental needs. As a proof-of-concept, flow-induced alignment of endothelial cells and the expression of shear-sensitive gene targets are demonstrated, and the different phases of neutrophil transmigration across a chemically stimulated endothelial monolayer under flow conditions are visualized. With these experimental capabilities, it is anticipated that both engineering and bioscience laboratories will adopt this reconfigurable design due to the compatibility with standard open-well protocols.

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Transwell Insert-Embedded Microfluidic Devices for Time-Lapse Monitoring of Alveolar Epithelium Barrier Function under Various Stimulations

Cited by 11 publications

References 34 publications

In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends

In Vitro Systems for Toxicity Evaluation of Microbial Volatile Organic Compounds on Humans: Current Status and Trends

Easy-to-Build and Reusable Microfluidic Device for the Dynamic Culture of Human Bronchial Cystic Fibrosis Epithelia

The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow

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