Trapping and SERS identification of extracellular vesicles using nanohole arrays

Kaufman, Lauren; Cooper, Tyler T.; Wallace, Gregory Q.; Hawke, David Connor; Betts, Dean H.; Hess, David A.; Lagugné‐Labarthet, François

doi:10.1117/12.2506633

Cited by 11 publications

(11 citation statements)

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“…14c ) 271 . In 2019, Lagugné-Labarthet et al proposed the use of plasmonic-well-based structures as a means of isolating, trapping and controlling the position of biologically relevant vesicles on plasmonic platforms 272 . These plasmonic devices provide a practical platform for trapping and identification of extracellular vesicles without the use of labelling agents, allowing characterization of the molecular details of individual extracellular vesicles.…”

Section: Applications Of Plasmonic Tweezersmentioning

confidence: 99%

Plasmonic tweezers: for nanoscale optical trapping and beyond

et al. 2021

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Optical tweezers and associated manipulation tools in the far field have had a major impact on scientific and engineering research by offering precise manipulation of small objects. More recently, the possibility of performing manipulation with surface plasmons has opened opportunities not feasible with conventional far-field optical methods. The use of surface plasmon techniques enables excitation of hotspots much smaller than the free-space wavelength; with this confinement, the plasmonic field facilitates trapping of various nanostructures and materials with higher precision. The successful manipulation of small particles has fostered numerous and expanding applications. In this paper, we review the principles of and developments in plasmonic tweezers techniques, including both nanostructure-assisted platforms and structureless systems. Construction methods and evaluation criteria of the techniques are presented, aiming to provide a guide for the design and optimization of the systems. The most common novel applications of plasmonic tweezers, namely, sorting and transport, sensing and imaging, and especially those in a biological context, are critically discussed. Finally, we consider the future of the development and new potential applications of this technique and discuss prospects for its impact on science.

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Section: Applications Of Plasmonic Tweezersmentioning

confidence: 99%

Plasmonic tweezers: for nanoscale optical trapping and beyond

et al. 2021

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“…For these reasons, they have been largely preferred for biosensing applications over direct approaches. Nonetheless, the current literature survey on exosome SERS analysis shows a larger number of reports based on direct approaches [ 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] than indirect ones [ 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 ]. This apparent anomaly may be explained by taking into considerations the intrinsic exosomal heterogeneity that currently burdens the identification of specific disease-related biomarkers for selective separation and labelling of clinically relevant exosome subpopulations [ 90 , 99 , 102 ].…”

Section: Isolation and Characterization Of Exosomesmentioning

confidence: 99%

Surface-Enhanced Raman Scattering (SERS) Spectroscopy for Sensing and Characterization of Exosomes in Cancer Diagnosis

Guerrini

Garcia‐Rico

O’Loghlen

et al. 2021

Cancers

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Exosomes are emerging as one of the most intriguing cancer biomarkers in modern oncology for early cancer diagnosis, prognosis and treatment monitoring. Concurrently, several nanoplasmonic methods have been applied and developed to tackle the challenging task of enabling the rapid, sensitive, affordable analysis of exosomes. In this review, we specifically focus our attention on the application of plasmonic devices exploiting surface-enhanced Raman spectroscopy (SERS) as the optosensing technique for the structural interrogation and characterization of the heterogeneous nature of exosomes. We summarized the current state-of-art of this field while illustrating the main strategic approaches and discuss their advantages and limitations.

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“…We have recently demonstrated that the secretome of Panc-MSC contains vesicle-like structures which harbour cargoes including proteins, lipids, and nucleic using custom fabricated nanohole trapping and surface enhanced Raman spectrometry 22 . Ultrafiltration was used to concentrate CM; however it remains unknown if this method could be modified to effectively segregate EVs from EV-independent cargo within the secretome of Panc-MSC.…”

Section: Ultrafiltration Enriched For Evs Generated By Human Panc-mscmentioning

confidence: 99%

“…It is well established the secretome of MSC is enriched with bioactive stimuli, derived from amino acids, lipids or nucleic acids, that mediate regenerative processes within target cell populations. 14,22 . Notably, EVs provide regenerative stimuli protection within a lipid bi-layer protected from enzymatic degradation.…”

Section: Introductionmentioning

confidence: 99%

Ultrafiltration Segregates Tissue Regenerative Stimuli Harboured Within and Independent of Extracellular Vesicles

Cooper

Sherman

Dayarathna

et al. 2020

Preprint

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The release of extracellular vesicles (EV) from human multipotent stromal cells (MSC) has been proposed as a mechanism by which MSC mediate regenerative functions in vivo. Our recent work has characterized MSC derived from human pancreatic tissues (Panc-MSC) that generated a tissue regenerative secretome which could be used as an injectable biotherapeutic agent in vivo. Despite these advancements, it remains unknown whether tissue regenerative stimuli released by Panc-MSC are released independent or within extracellular vesicles (EVs).Herein, this study demonstrates ultrafiltration is a simple method to enrich for EVs which can be injected in murine models of blood vessel or pancreatic islet regeneration. The enrichment of EVs from Panc-MSC conditioned media (CM) was validated using nanoscale flow cytometry and atomic force microscopy; in addition to the exclusive detection of classical EV-markers CD9, CD81, CD63 using label-free mass spectrometry. Additionally, we identified several proregenerative stimuli, such as WNT5A or ANGPT1, exclusively detected in Panc-MSC EVenriched versus EV-depleted conditioned media. Human microvascular endothelial cell tubule formation was enhanced in response to both Panc-MSC CM fractions in vitro yet only intramuscular injection of EV-enriched CM demonstrated vascular regenerative functions in NOD/SCID mice with unilateral hind-limb ischemia (*

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Trapping and SERS identification of extracellular vesicles using nanohole arrays

Cited by 11 publications

References 0 publications

Plasmonic tweezers: for nanoscale optical trapping and beyond

Plasmonic tweezers: for nanoscale optical trapping and beyond

Surface-Enhanced Raman Scattering (SERS) Spectroscopy for Sensing and Characterization of Exosomes in Cancer Diagnosis

Ultrafiltration Segregates Tissue Regenerative Stimuli Harboured Within and Independent of Extracellular Vesicles

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