Trapping of phosphate solubilizing bacteria on hyphae of the arbuscular mycorrhizal fungus Rhizophagus irregularis DAOM 197198

Taktek, Salma; Trépanier, Martin; Servin, Paola Magallon; St‐Arnaud, Marc; Piché, Yves; Fortin, J. A.; Antoun, Hani

doi:10.1016/j.soilbio.2015.07.016

Cited by 87 publications

(55 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Results indicated that the optimum biofilm formation was obtained in flat bottom 96- or 6-well polystyrene microtiter plates after 5 days incubation at 28 °C without agitation. This period of time also allowed maximum P mobilization from Quebec PR ground to 150 μm (Taktek et al 2015) or hydroxyapatite. All tests were performed in a completely randomized block design with four to nine blocks as specified below by using as sole P source either the low reactive Quebec igneous PR (total P 146.44 mg g −1 ; 4.97 mg g −1 soluble in 2 % formic acid; and 7.05 mg g −1 soluble in 2 % citric acid) or the hydroxyapatite, a synthetic micropowder of calcium apatite (Sigma-Aldrich; 20–50 μm) used as a control for its high reactivity.…”

Section: Methodsmentioning

confidence: 98%

“…Rs11 (GenBank KC241900) and B. phenazinium Bph12 (GenBank KC241901) isolated from the mycorrhizosphere of different mycorrhizal plants (Taktek et al 2015). Spores of Ri DAOM 197198 were kindly supplied by Premier Tech, Rivière-du-Loup, Québec, Canada.…”

Section: Methodsmentioning

confidence: 99%

“…After 5 days of incubation at 28 °C without agitation on modified NBRIP, aliquots were removed from each well to determine the amount of soluble P released and the pH. The amount of soluble P was determined as described by Taktek et al (2015). Briefly, 100 μL of the supernatant was diluted in an appropriate volume of distilled water and mixed with 100 μL of 5.5 % ( w / v ) trichloroacetic acid (Sigma-Aldrich) and incubated for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…We have recently described a method to isolate phosphate-solubilizing hyphobacteria strongly attached to the hyphae of the AM fungus Rhizoglomus irregulare DAOM 197198 ( Ri ; formerly Glomus irregulare or Rhizophagus irregularis ; Sieverding et al 2014) and observed that in a two-compartment Petri plate system, the extraradical Ri hyphal exudates supported the growth of PSB (Taktek et al 2015). Under these experimental conditions, Ri hyphae mobilized very little P from PR; however, in the presence of PSB, substantially more P was mobilized.…”

Section: Introductionmentioning

confidence: 99%

“…Since hyphobacteria strongly attached to Ri DAOM 197198 hyphae solubilized more efficiently a low reactive igneous PR from Québec Canada than loosely attached mycorrhizobacteria (Taktek et al 2015), we hypothesized that the superiority of hyphobacteria can be attributed to their ability to form important biofilms with high P mobilizing activity. Here, we describe bacterial biofilms formed on abiotic surfaces by hyphobacteria trapped on Ri hyphae and by mycorrhizobacteria isolated from roots of different mycorrhizal plants, and we compare their P mobilizing activities from a Quebec PR or hydroxyapatite.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Igneous phosphate rock solubilization by biofilm-forming mycorrhizobacteria and hyphobacteria associated with Rhizoglomus irregulare DAOM 197198

et al. 2016

Self Cite

View full text Add to dashboard Cite

Biofilm formation on abiotic and biotic surfaces was studied with two hyphobacteria, strongly attached to the surface of the arbuscular mycorrhizal fungus (AMF) Rhizoglomus irregulare (Ri) DAOM 197198 and two mycorrhizobacteria, loosely attached to the roots of different mycorrhizal plants. When the sparingly soluble igneous phosphate rock (PR) from Quebec, or when the chemical hydroxyapatite were used as sole phosphorus (P) source, hyphobacteria Rhizobium miluonense Rm3 and Burkholderia anthina Ba8 produced significantly more biofilms than mycorrhizobacteria Rahnella sp. Rs11 and Burkholderia phenazinium Bph12, as indicated by the crystal violet assay or by quantifying biofilm exopolysaccharides. As previously observed with planktonic bacteria, biofilms mobilized P by lowering the pH and releasing gluconic acid. The high efficiency of P mobilization by the hyphobacteria Ba8 was linked to the presence of more viable cells in its biofilm as revealed by the hydrolysis of fluorescein diacetate. Scanning electron microscopy micrographs showed a high adherence of the best P-solubilizer hyphobacteria Ba8 on the surface of Quebec PR. Hydroxyapatite porous structure did not allow a good adherence of Ba8. Ba8 formed an important biofilm on the hyphae of Ri DAOM 197198 with low reactive Quebec PR while no biofilm was observed with the high reactive hydroxyapatite. Results confirm the possible presence of specificity between the Ri DAOM 197198 and the hyphobacteria and suggest that the interaction would be regulated by the availability of P.Electronic supplementary materialThe online version of this article (doi:10.1007/s00572-016-0726-z) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Igneous phosphate rock solubilization by biofilm-forming mycorrhizobacteria and hyphobacteria associated with Rhizoglomus irregulare DAOM 197198

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

Spatial differences in soil microbial diversity caused by pH ‐driven organic phosphorus mineralization

et al. 2020

View full text Add to dashboard Cite

Microbial diversity response to abiotic and biotic factors provides a sensitive indicator for estimating the potential stability and degradation of soils in agro‐ecosystems. To determine the effects of pH on organic phosphorus mineralization and microbial diversity, phospholipid fatty acid analysis, quantitative polymerase chain reaction (qPCR), and multiple ecological analyses were performed. Significant correlations were found between phosphorus components and alkaline phosphatase, phytase, and pH, between phoD and phytase, between bpp and alkaline phosphatase. phoD and bpp gene abundance presented significant linear relationships with soil pH and microbial diversity. Abiotic and biotic factors explained 25.1% of the total variation in organic phosphorus‐mineralizing‐related gene abundance, and abiotic factors accounted for 13.2% of the total variation in microbial community composition. Soil pH was the determinant, accounting for 11.2 and 7.7% of the total variation in organic phosphorus‐mineralizing‐related gene abundance and microbial community composition, respectively. Our results emphasized that the phosphorus components, pH, and organic phosphorus‐mineralizing‐related gene abundance were responsible for organic phosphorus‐mineralizing‐related enzyme activity. To our knowledge, this is the first report that pH is a key factor in directly and indirectly determining organic phosphorus‐mineralizing‐related gene abundance, which in turn affects microbial diversity, on a large spatial scale. The differences in phosphorus components, enzyme activity, organic phosphorus‐mineralizing‐related gene abundance, microbial community composition and diversity caused by pH might explain crop yield reduction.

show abstract

Carbon Fluxes in Mycorrhizal Plants

Řezáčová

Konvalinková

Jansa

2017

Mycorrhiza - Eco-Physiology, Secondary Metabolites, Nanomaterials

View full text Add to dashboard Cite

Trapping of phosphate solubilizing bacteria on hyphae of the arbuscular mycorrhizal fungus Rhizophagus irregularis DAOM 197198

Cited by 87 publications

References 47 publications

Igneous phosphate rock solubilization by biofilm-forming mycorrhizobacteria and hyphobacteria associated with Rhizoglomus irregulare DAOM 197198

Igneous phosphate rock solubilization by biofilm-forming mycorrhizobacteria and hyphobacteria associated with Rhizoglomus irregulare DAOM 197198

Spatial differences in soil microbial diversity caused by pH ‐driven organic phosphorus mineralization

Carbon Fluxes in Mycorrhizal Plants

Contact Info

Product

Resources

About