We report production rates for H 2 O and eight trace molecules (CO, C 2 H 6 , CH 4 , CH 3 OH, NH 3 , H 2 CO, HCN, C 2 H 2 ) in the dynamically new, Sun-grazing Comet C/2012 S1 (ISON), using high-resolution spectroscopy at Keck II and the NASA IRTF on 10pre-perihelion dates encompassing heliocentric distances R h =1.21-0.34 AU. Measured water production rates spanned two orders of magnitude, consistent with a long-term heliocentric power law) . Abundance ratios for CO, C 2 H 6 , and CH 4 with respect to H 2 O remained constant with R h and below their corresponding mean values measured among a dominant sample of Oort Cloud comets. CH 3 OH was also depleted for R h > 0.5 AU, but was closer to its mean value for R h ≤0.5 AU. The remaining four molecules exhibited higher abundance ratios within 0.5 AU: for R h > 0.8 AU, NH 3 and C 2 H 2 were consistent with their mean values while H 2 CO and HCN were depleted. For R h < 0.5 AU, all four were enriched, with NH 3 , H 2 CO, and HCN increasing most. Spatial profiles of gas emission in ISON consistently peaked sunward of the dust continuum, which was asymmetric antisunward and remained singly peaked for all observations. NH 3 within 0.5 AU showed a broad spatial distribution, possibly indicating its release in the coma provided thatoptical depth effects were unimportant. The column abundance ratio NH 2 /H 2 O at 0.83 AU was close to the "typical" NH/OH from optical wavelengths, but was higher within 0.5 AU. Establishing its production rate and testing its parentage (e.g., NH 3 ) requiremodeling of coma outflow.