Trauma-induced opening of the the blood-spinal cord barrier is reduced by indomethacin, an inhibitor of prostaglandin biosynthesis. Experimental observations in the rat using [131I]-sodium, Evans blue and lanthanum as tracers

Sharma, Hari Shanker; Olsson, Yngve; Persson, Sabine Gebert

doi:10.3233/rnn-1995-7403

Cited by 14 publications

(29 citation statements)

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“…BSCB permeability was examined using either Evans blue and/or [131] I-sodium in the perifocal T9 and T12 segments. 17,20 The intravascular tracer was washed out by a brief saline rinse through the heart. 20 Tissue pieces from T9, T10-11, and T12 were dissected out, weighed immediately, and counted in a 3-inch-well type gamma counter (energy window: 500-800 keV).…”

Section: Bscb Permeabilitymentioning

confidence: 99%

“…17,20 The intravascular tracer was washed out by a brief saline rinse through the heart. 20 Tissue pieces from T9, T10-11, and T12 were dissected out, weighed immediately, and counted in a 3-inch-well type gamma counter (energy window: 500-800 keV). After counting the radioactivity, tissue samples were processed for Evans blue measurement using colorimetric techniques.…”

Section: Bscb Permeabilitymentioning

confidence: 99%

“…After counting the radioactivity, tissue samples were processed for Evans blue measurement using colorimetric techniques. 17,20…”

Section: Bscb Permeabilitymentioning

confidence: 99%

“…16 The water content of the cord was calculated from the differences in the wet and dry weights as described earlier. 20…”

Section: Edema Formationmentioning

confidence: 99%

“…The passage of tracer transfer across the BSCB was examined using extravasation of lanthanum. 20 Animals were perfused with fixative containing lanthanum: lanthanum chloride (2.5%). 16,20 Lanthanum is an electron dense tracer seen as black particle within the lumen at the ultrastructural level.…”

Section: Lanthanum Extravasationmentioning

confidence: 99%

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Neuroprotective Effects of Neurotrophins and Melanocortins in Spinal Cord Injury: An Experimental Study in the Rat Using Pharmacological and Morphological Approaches

Sharma¹

2005

Annals of the New York Academy of Sciences

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Spinal cord injury (SCI) induces lifetime disability, and no suitable therapy is available to treat victims or to minimze their sufferings. Recently, neurotrophins and compounds acting at melanocortin receptors have been been identified as potential neuroprotective agents. In this investigation, the neuroprotective effects of neurotrophins and melanocortins on the pathophysiology of SCI were examined in a rat model. The SCI was produced by making a longitudinal incision into the right dorsal horn of the T10-11 segments under equithesin anesthesia. In separate groups, neurotrophins [BDNF or IGF-1 (0.1-1 microg/10 microL in saline)] or melanocortins (ME10092, ME10354, ME10393, ME10431, and ME10501, having affinities to melanocortin receptors; 1-10 mug in saline) were applied topically over the traumatized cord segment within 5-10 min after SCI and the rats were allowed to survive for 5 h. A focal SCI resulted in widespread disruption of the blood-spinal cord barrier (BSCB) to Evans blue albumin (EBA), ([131])iodine, or lanthanum tracers and exhibited profound edema formation and cell or tissue destruction. Topical application of BDNF, IGF-1, or ME10501 (having high affinity to melanocortin-4 receptor, MCR-4) in high quantity markedly attenuated BSCB disruption, edema formation, and nerve cell, glial cell, and axonal injuries. On the other hand, low doses of neurotrophins or melanocortins were not effective in attenuating pathophysiology of SCI. These observations suggest that neurotrophins (BDNF and IGF-1) and melanocortins (with high affinity to MCR-4) are capable of inducing neuroprotection if applied shortly after trauma in high doses. Taken together, the results indicate that neurotrophins and melanocortins participate in the pathophysiology of spinal cord cell and tissue injury following trauma.

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Section: Bscb Permeabilitymentioning

confidence: 99%

Section: Bscb Permeabilitymentioning

confidence: 99%

“…After counting the radioactivity, tissue samples were processed for Evans blue measurement using colorimetric techniques. 17,20…”

Section: Bscb Permeabilitymentioning

confidence: 99%

“…16 The water content of the cord was calculated from the differences in the wet and dry weights as described earlier. 20…”

Section: Edema Formationmentioning

confidence: 99%

Section: Lanthanum Extravasationmentioning

confidence: 99%

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Neuroprotective Effects of Neurotrophins and Melanocortins in Spinal Cord Injury: An Experimental Study in the Rat Using Pharmacological and Morphological Approaches

Sharma¹

2005

Annals of the New York Academy of Sciences

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Low molecular weight compounds with affinity to melanocortin receptors exert neuroprotection in spinal cord injury — an experimental study in the rat

Sharma

Lundstedt²,

Flärdh³

et al. 2003

Brain Edema XII

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A Bradykinin BK2 Receptor Antagonist HOE-140 Attenuates Blood-Spinal Cord Barrier Permeability Following a Focal Trauma to the Rat Spinal Cord

Sharma

2000

Brain Edema XI

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Trauma-induced opening of the the blood-spinal cord barrier is reduced by indomethacin, an inhibitor of prostaglandin biosynthesis. Experimental observations in the rat using [131I]-sodium, Evans blue and lanthanum as tracers

Cited by 14 publications

References 0 publications

Neuroprotective Effects of Neurotrophins and Melanocortins in Spinal Cord Injury: An Experimental Study in the Rat Using Pharmacological and Morphological Approaches

Neuroprotective Effects of Neurotrophins and Melanocortins in Spinal Cord Injury: An Experimental Study in the Rat Using Pharmacological and Morphological Approaches

Low molecular weight compounds with affinity to melanocortin receptors exert neuroprotection in spinal cord injury — an experimental study in the rat

A Bradykinin BK2 Receptor Antagonist HOE-140 Attenuates Blood-Spinal Cord Barrier Permeability Following a Focal Trauma to the Rat Spinal Cord

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