Police officers are frequently confronted with various stressors that may affect their mental health. Psychological resilience may protect against these effects. For this purpose, a Mental Strength Training (MST) was developed by the Dutch Police Academy aimed at psychological resilience enhancement. The present three-wave study examined efficacy of this training using a quasiexperimental study design among police officers (N Total = 305, n Experimenal = 138, n Comparison = 167). Additionally, we compared between officers in the experimental and comparison group recently confronted with a potentially traumatic event (N Total = 170, n Experimenal = 74, n Comparison = 96). Questionnaires on resilience (Mental Toughness Questionnaire-48 (MTQ-48) and Resilience Scale-nl (RS-nl)), mental health disturbances (Symptoms CheckList 90-R (SCL-90-R) and Self-Rating Inventory for PTSD (SRIP)), were administered pre-training, and about 3 and 9 months post-training. Mixed-effects models showed training effects on Interpersonal Confidence. Similar analyses among officers with recent potentially traumatic event experience showed significant training effects for the RS-nl subscale of Acceptance of Self and Life, MTQ-48 total score, and the MTQ-48 subscale of Interpersonal Confidence. However, all effects yielded small effect sizes according to Cohen's d, and are therefore of limited practical relevance. Officer's appraisal of training benefits on resilience enhancement was largely negative. We found no indications that 4-day training substantially improved officer's psychological resilience or mental health.