Traumatic injury pattern is of equal relevance as injury severity for experimental (poly)trauma modeling

Yang, Bing; Bundkirchen, Katrin; Krettek, Christian; Relja, Borna; Neunaber, Claudia

doi:10.1038/s41598-019-42085-1

Cited by 12 publications

(25 citation statements)

References 71 publications

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“…However, considering the definition of polytrauma according to the ISS or the new Berlin Group allocation and experimental model. The experimental setting was described before 30 . Prior to experimental procedures randomization of mice were performed to one of ten groups.…”

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confidence: 99%

“…The multi-trauma (MT) group received HS, Lap, TxT, and bsTT was included instead of Fx. The injury severity was calculated as described before [29][30][31] in all multiple trauma groups according to the ISS system. The group allocation with animal number is shown in Table 1.…”

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confidence: 99%

“…Osteotomy with external fixation (Fx) was induced as described before 30 . Here, a longitudinal approach of the skin was performed followed by the opening of the tensor fasciae latae.…”

mentioning

confidence: 99%

“…the vastus lateralis biceps femoris muscles were split bluntly. Afterwards, MouseExFix simple L 100% external fixator system (RISystem AG, Davos, Switzerland) was attached to the femur 24,30,25 . Subsequent osteotomy was induced by a 0.44 mm Gigli wire saw (RISystem AG) at the middle of the femur.…”

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confidence: 99%

“…Afterwards, the fascia lata was closed with continuous and the skin with interrupted sutures. As described before laparotomy (Lap) was performed in a supine position 30 . 1 ml of preheated 0.9% sodium chloride solution was provided into the cavity.…”

mentioning

confidence: 99%

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Different experimental multiple trauma models induce comparable inflammation and organ injury

Relja

Yang

Bundkirchen

et al. 2020

Sci Rep

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Multiple injuries appear to be a decisive factor for experimental polytrauma. Therefore, our aim was to compare the inflammatory response and organ damage of five different monotrauma with three multiple trauma models. For this, mice were randomly assigned to 10 groups: Healthy control (Ctrl), Sham, hemorrhagic shock (HS), thoracic trauma (TxT), osteotomy with external fixation (Fx), bilateral soft tissue trauma (bsTT) or laparotomy (Lap); polytrauma I (PT I, TxT + HS + Fx), PT II (TxT + HS + Fx + Lap) and one multi-trauma group (MT, TxT + HS + bsTT + Lap). The inflammatory response and organ damage were quantified at 6 h by analyses of IL-6, IL-1β, IL-10, CXCL1, SAA1, HMGB1 and organ injury. Systemic IL-6 increased in all mono and multiple trauma groups, while CXCL1 increased only in HS, PT I, PT II and MT vs. control. Local inflammatory response was most prominent in HS, PT I, PT II and MT in the liver. Infiltration of inflammatory cells into lung and liver was significant in all multiple trauma groups vs. controls. Hepatic and pulmonary injury was prominent in HS, PT I, PT II and MT groups. These experimental multiple trauma models closely mimic the early post-traumatic inflammatory response in human. Though, the choice of read-out parameters is very important for therapeutic immune modulatory approaches.

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confidence: 99%

mentioning

confidence: 99%

“…Osteotomy with external fixation (Fx) was induced as described before 30 . Here, a longitudinal approach of the skin was performed followed by the opening of the tensor fasciae latae.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Different experimental multiple trauma models induce comparable inflammation and organ injury

Relja

Yang

Bundkirchen

et al. 2020

Sci Rep

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A randomized controlled trial of simulation‐based mastery learning to teach the extended focused assessment with sonography in trauma

Smith

Lobo

Anderson

et al. 2021

AEM Education and Training

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Background: Mastery learning has gained popularity for training residents in procedural skills due to its demonstrated superiority over traditional methods. However, no studies have compared the efficacy of traditional versus mastery learning methods in residency point-of-care ultrasound education. We hypothesized that mastery learning would improve residents' skills in performing the extended focused assessment with sonography in trauma (eFAST).Methods: All first-year emergency medicine (EM) resident physicians at a single university hospital underwent a crossover randomized controlled trial to receive masterylearning eFAST training either at the beginning of the academic year or 6 months into intern year. Participants were taught using a checklist validated by a panel of experts using Mastery Angoff methods and were given feedback on missed tasks until each trainee completed the eFAST with a minimum passing standard (MPS). Our primary outcome was technical proficiency between the two groups for eFAST examinations performed in the emergency department during the academic year.Results: Sixteen interns were enrolled; eight were randomized to each group. The group that received mastery training at the beginning of the year had mean clinical eFAST proficiency scores above the MPS in the first two quarters of the academic year, while the control group did not. Once the control group underwent eFAST mastery training at the midpoint of the year, both groups had mean proficiency scores above the MPS for the remainder of the year. Conclusion:Simulation-based mastery learning is an effective method of teaching the eFAST examination. This training during intern orientation conferred early proficiency in clinical performance of eFAST among EM residents. This difference in proficiency was no longer present after the control group received mastery learning education halfway through the academic year.

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A novel model of urosepsis in mice developed by ureteral ligation and injection of Escherichia coli into the renal pelvis

Hu,

Yan,

Tang

et al. 2024

Heliyon

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Traumatic injury pattern is of equal relevance as injury severity for experimental (poly)trauma modeling

Cited by 12 publications

References 71 publications

Different experimental multiple trauma models induce comparable inflammation and organ injury

Different experimental multiple trauma models induce comparable inflammation and organ injury

A randomized controlled trial of simulation‐based mastery learning to teach the extended focused assessment with sonography in trauma

A novel model of urosepsis in mice developed by ureteral ligation and injection of Escherichia coli into the renal pelvis

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