Traumatic stress‐induced persistent changes in <scp>DNA</scp> methylation regulate neuropeptide Y expression in rat jejunum

Sagarkar, Sneha; Mahajan, Supriya D.; Choudhary, Amit; Borkar, Chandrashekhar D.; Kokare, Dadasaheb M.; Sakharkar, Amul J.

doi:10.1111/nmo.13074

Cited by 12 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gastrointestinal function is closely related to the level of fatigue in CFS patients. Relevant research shows that the patient with CFS has intestinal barrier dysfunction, gut immune dysfunction, altered gut motility, altered gastrointestinal secretion, altered intestinal flora, and altered gut-brain axis [35,36].These changes make CFS patients prone to diarrhea, abdominal pain, constipation and other digestive symptoms [37][38][39][40][41]. At the same time, some studies found that abnormal alteration to intestinal flora are linearly related to decreased motivation, increased levels of fatigue, anxiety, depression in CFS patient [42,43].…”

Section: Discussionmentioning

confidence: 99%

Effect of Prolong-life-with-nine-turn-method (Yan Nian Jiu Zhuan) Qigong on fatigue and gastrointestinal function in patients with chronic fatigue syndrome: Study protocol for a randomized controlled trial

Gu,

You,

Guo

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Introduction Chronic fatigue syndrome (CFS) is a physical and mental disorder in which long-term fatigue is the main symptom. CFS patients are often accompanied by functional gastrointestinal diseases (FGIDs), which lead to decreased quality of life and increased fatigue. Prolong-life-with-nine-turn-method (PLWNT) is a kind of physical and mental exercise. Its operation includes adjusting the mind, breathing and cooperating with eight self-rubbing methods and one upper body rocking method. PLWNT was used to improve the digestive function in ancient China and to treat FGIDs such as functional dyspepsia and irritable bowel syndrome in modern times. Previous studies have shown that PLWNT can reduce fatigue in patients with CFS. But it is unclear whether the effect of PLWNT on CFS fatigue is related to gastrointestinal function. The aim of this study was to explore the relationship between PLWNT and fatigue and gastrointestinal function in patients with CFS. Methods This study is a non-inferiority randomized controlled trial (RCT). The whole study period is 38 weeks, including 2 weeks of baseline evaluation, 12 weeks of intervention and 6 months of follow-up. Ninety-six CFS patients will be stratified random assigned to the intervention group (PLWNT) and the control group (cognitive behavior treatment) in the ratio of 1:1 through the random number table generated by SPSS. In the evaluation of results, Multidimensional Fatigue Inventory-20 (MFI-20), Gastrointestinal Symptom Rating Scale (GSRS), Bristol Stool Form Scale (BSFS), and Short Form 36 item health survey (SF-36) will be evaluated at week 0 (baseline), week 6 (midterm), week 12 (endpoint) and month 9 (follow up). The intestinal flora will be evaluated at week 0 (baseline) and week 12 (endpoint). The data results will be processed by statistical experts. The data analysis will be based on the intention to treat principle and per-protocol analysis. In the efficacy evaluation, repeated measurement analysis of variance will be used for data conforming to normal distribution or approximate normal distribution. The data which do not conform to the analysis of repeated measurement variance will be analyzed by the generalized estimation equation Linear discriminant analysis will be used to clarify the difference species of intestinal flora. The significance level sets as 5%. The safety of interventions will be evaluated after each treatment session. Discussion This trial will provide evidence to PLWNT exerting positive effects on fatigue and gastrointestinal function of CFS. It will further explore whether the improvement of PLWNT on CFS fatigue is related to gastrointestinal function. Trial registration The trial was registered at Chinese Clinical Trial Registry http://www.chictr.org.cn/showproj.aspx?proj=151456 (Registration No.: ChiCTR2200056530). Date: 2022-02-07.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Prolong-life-with-nine-turn-method (Yan Nian Jiu Zhuan) Qigong on fatigue and gastrointestinal function in patients with chronic fatigue syndrome: Study protocol for a randomized controlled trial

Gu,

You,

Guo

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The rat model with closed head diffused brain injury was employed to induce rMTBI, as described previously [9,14,15,44]. To induce rMTBI, the rats were subjected to 5 hits of MTBI on alternate days over 9 days.…”

Section: Repeated Mild Traumatic Brain Injurymentioning

confidence: 99%

“…The mRNA expression of the HDACs, CBP, and CREB was estimated using RT-qPCR, as described previously [14,15,44]. The total RNA from the Arc tissues was isolated using the TRIzol (Ambion, Austin, TX, USA) method, and the DNA contamination was removed using the DNA-free TM DNA Removal Kit (Life Technologies, Carlsbad, CA, USA).…”

Section: Rt-qpcr For Mrna Measurementsmentioning

confidence: 99%

See 1 more Smart Citation

Role for Histone Deacetylation in Traumatic Brain Injury-Induced Deficits in Neuropeptide Y in Arcuate Nucleus: Possible Implications in Feeding Behavior

et al. 2020

View full text Add to dashboard Cite

Introduction: Repeated traumatic events result in long-lasting neuropsychiatric ailments, including neuroendocrine imbalances. Neuropeptide Y (NPY) in the arcuate nucleus (Arc) is an important orexigenic peptide. However, the molecular underpinnings of its dysregulation owing to traumatic brain injury remain unknown. Methods: Rats were subjected to repeated mild traumatic brain injury (rMTBI) using the closed-head weight drop model. Feeding behaviour and the regulatory epigenetic parameters of NPY expression were measured at 48 hrs and 30 days post rMTBI. Further, sodium butyrate, a pan-HDAC inhibitor, was administered to examine whether histone deacetylation is involved in NPY expression post rMTBI. Results: The rMTBI attenuated food intake, which was coincident with a decrease in NPY mRNA and protein levels in the Arc post rMTBI. Further, rMTBI also reduced the mRNA levels of the cAMP response element-binding protein (CREB) and CREB-binding protein (CBP) and altered the mRNA levels of the various isoforms of the histone deacetylases (HDACs). Concurrently, the acetylated H3-K9 levels and the binding of CBP at the NPY promoter in the Arc of the rMTBI-exposed rats were reduced. However, the treatment with sodium butyrate corrected the rMTBI-induced deficits in the H3-K9 acetylation levels and CBP occupancy at the NPY promoter, restoring both the NPY expression and the food intake. Conclusions: These findings suggest that histone deacetylation at the NPY promoter persistently controls NPY function in the Arc after rMTBI. This study also demonstrates the efficacy of HDAC inhibitors in mitigating trauma-induced neuroendocrine maladaptations in the hypothalamus.

show abstract

“…Several psychological and physiological disturbances following TBI are known to be sufficient to alter the balance of gut homeostasis maintained by the microbial ecosystem and host immune system, with results including traumatic stress 5 , dysfunctional intestinal contractility and motility 6,7 , and increased gut permeability 8 . Accordingly, animal studies have revealed profound changes in gut microbiota after various nerve injuries 6,7 , such as stroke spinal cord injury 8 and TBI 4,5 . More importantly, gut dysbiosis in animal models is not merely a byproduct of injury but an essential contributory factor to TBI-related neuropathology and impaired behavioral outcomes 9 .…”

Section: Introductionmentioning

confidence: 99%

Association of gut microbiota with cerebral cortex and cerebrovascular abnormality in human mild traumatic brain injury

Bai

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Key roles of the gut−brain axis in brain injury development have been suggested in various mouse models; however, little is known about its functional significance in human mild traumatic brain injury (TBI). Here, we deciphered this axis by profiling the gut microbiota in 98 acute mild TBI patients and 62 matched controls, and subgroup of them also measured circulating mediators and applied neuroimaging. Mild TBI patients had increased α−diversity and different overall microbial compositions compared with controls. 25−microbial genus classifiers distinguished patients from controls with an area under the receiver operating characteristic curve (AUC) of 0.889, while adding serum mediators and neuroimaging features further improved performance even in a small sample size (AUC = 0.969). Numerous correlations existed between gut bacteria, aberrant cortical thickness and cerebrovascular injury. Co−occurrence network analysis revealed two unique gut−brain axes in patients: 1) altered intestinal Lachnospiraceae_NK4A136_group and Eubacterium_ruminantium_group-increased serum GDNF−subcallosal hypertrophy and cerebrovascular injury; 2) decreased intestinal Eubacterium_xylanophilum_group−upregulated IL−6−thinned anterior insula. Our findings provided a new integrated mechanistic understanding and diagnostic model of mild TBI.

show abstract

Traumatic stress‐induced persistent changes in DNA methylation regulate neuropeptide Y expression in rat jejunum

Abstract: These results suggest that DNA demethylation could be operative as an epigenetic mechanism in the long-term regulation of NPY gene expression to alter the gut motility during traumatic stress.

Cited by 12 publications

References 54 publications

Effect of Prolong-life-with-nine-turn-method (Yan Nian Jiu Zhuan) Qigong on fatigue and gastrointestinal function in patients with chronic fatigue syndrome: Study protocol for a randomized controlled trial

Effect of Prolong-life-with-nine-turn-method (Yan Nian Jiu Zhuan) Qigong on fatigue and gastrointestinal function in patients with chronic fatigue syndrome: Study protocol for a randomized controlled trial

Role for Histone Deacetylation in Traumatic Brain Injury-Induced Deficits in Neuropeptide Y in Arcuate Nucleus: Possible Implications in Feeding Behavior

Association of gut microbiota with cerebral cortex and cerebrovascular abnormality in human mild traumatic brain injury

Contact Info

Product

Resources

About