Traumatic Wound Microbiome Workshop

Kirkup, Benjamin C.; Craft, David W.; Palys, Thomas J.; Black, Chad; Heitkamp, Rae A; Li, C.; Lu, Y.; Matlock, N.; McQueary, Christin N.; Michels, Aretha; Peck, George W.; Si, Yuanzheng; Summers, Amy; Thompson, Mitchell G.; Zurawski, Daniel V.

doi:10.1007/s00248-012-0070-6

Cited by 2 publications

(4 citation statements)

References 44 publications

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“…Tissue injury compromises barrier function, and increases risk of infection and sepsis. Recent observations from our laboratory indicate that ubiquitous MSCs can modulate systemic responses to bacterial infection and support tissue repair and healing when recruited at sites of injury [2,5,9,[14][15][16][17][18]. However, these "compensatory" responses of MSCs can be skewed and suppressed after irradiation.…”

Section: Resultsmentioning

confidence: 99%

“…The data presented in Fig. 1 indicate that, despite radiation-produced damage and suppression of proliferative activity, MSCs demonstrated substantial radioresistance and absence of significant apoptotic and necrotic transformations in a wide range of radiation doses, i.e., [1][2][3][4][5][6][7][8][9][10][11][12] Gy. Interactive investigation of the stress-response factors implicated in cell survival may be important for the development of effective therapies for radiation injury (RI).…”

Section: Alterations In the Msc Stress-response-proteins Following Irmentioning

confidence: 99%

“…Thus, mesenchymal stroma protects the body from infections [1][2][3][4][5][6][7][8]. A breach of immune and structural integrity of tissue barriers under patho-physiological conditions such as complicated injury can lead to translocation of bacteria from different host-associated microbiomes and colonization of vital organs that can ultimately result in multiple organ failure and sepsis [9].…”

Section: Introductionmentioning

confidence: 99%

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Up-Regulation of Autophagy Defense Mechanisms in Mouse Mesenchymal Stromal Cells in Response to Ionizing Irradiation Followed by Bacterial Challenge

Gorbunov¹,

Elliott²,

McDaniel³

et al. 2013

Autophagy - A Double-Edged Sword - Cell Survival or Death?

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Section: Resultsmentioning

confidence: 99%

Section: Alterations In the Msc Stress-response-proteins Following Irmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Up-Regulation of Autophagy Defense Mechanisms in Mouse Mesenchymal Stromal Cells in Response to Ionizing Irradiation Followed by Bacterial Challenge

Gorbunov¹,

Elliott²,

McDaniel³

et al. 2013

Autophagy - A Double-Edged Sword - Cell Survival or Death?

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“…Polymicrobial infection caused by multiple species of microorganisms belonging to markedly different taxonomic groups is a common occurrence in severely immunocompromised patients [1-5] as well as in individuals suffering from persistent diabetic wounds [6-9], chronic pulmonary obstructive disease [10-13], cystic fibrosis patients suffering from chronic infections [14-20] and lung transplant recipients [21-23]. The microorganisms more commonly isolated from mixed microbial infections are pathogenic bacteria and fungi.…”

Section: Introductionmentioning

confidence: 99%

Development and antimicrobial susceptibility studies of in vitro monomicrobial and polymicrobial biofilm models with Aspergillus fumigatus and Pseudomonas aeruginosa

2014

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BackgroundMixed microbial infections of the respiratory tracts with P. aeruginosa and A. fumigatus capable of producing biofilms are commonly found in cystic fibrosis patients. The primary objective of this study was to develop an in vitro model for P. aeruginosa and A. fumigatus polymicrobial biofilm to study the efficacy of various antimicrobial drugs alone and in combinations against biofilm-embedded cells. Simultaneous static cocultures of P. aeruginosa and sporelings were used for the development of in vitro P. aeruginosa-A. fumigatus polymicrobial biofilm in SD broth in 24-well cell culture plates at 35°C, and the biofilm formation was monitored microscopically and spectrophotometrically. Using P. aeruginosa-A. fumigatus sporelings cocultures we examined the effects of various antimicrobial drugs alone and in combination against polymicrobial biofilm by CFU and tetrazolium reduction assays.ResultsIn simultaneous static cocultures P. aeruginosa cells killed A. fumigatus conidia, whereas the bacterial cells showed no substantial fungicidal effect on sporelings grown for 12 h or longer at 35°C. Monospecies cultures of P. aeruginosa produced loosely adhered monomicrobial biofilm and addition of 10% bovine serum to the growth medium inhibited the formation of monomicrobial biofilm by P. aeruginosa whereas it produced tightly adhered polymicrobial biofilm in the presence of A. fumigatus mycelial growth. A. fumigatus produced firmly adherent monomicrobial and polymicrobial biofilms. A comparison of CFU and MTT assays showed that the latter is unsuitable for studying the effectiveness of antimicrobial treatment against polymicrobial biofilm. Tobramycin alone and in combination with posaconazole was highly effective against monomicrobial and polymicrobial biofilms of P. aeruginosa whereas cefepime alone and in combination with posaconazole showed excellent activity against monomicrobial biofilm of P. aeruginosa but was less effective against polymicrobial biofilm. Monomicrobial and polymicrobial biofilms of A. fumigatus showed similar susceptibility to posaconazole with and without the antibacterial drug.ConclusionsSimultaneous static coculture of A. fumigatus sporelings grown for 12 h or longer was superior to ungerminated conidia with P. aeruginosa for the development of A. fumigatus-P. aeruginosa biofilm. P. aeruginosa-A. fumigatus polymicrobial biofilm shows differential susceptibility to antimicrobial drugs whereas the susceptibility of A. fumigatus to antimicrobial drugs was unchanged.

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Traumatic Wound Microbiome Workshop

Cited by 2 publications

References 44 publications

Up-Regulation of Autophagy Defense Mechanisms in Mouse Mesenchymal Stromal Cells in Response to Ionizing Irradiation Followed by Bacterial Challenge

Up-Regulation of Autophagy Defense Mechanisms in Mouse Mesenchymal Stromal Cells in Response to Ionizing Irradiation Followed by Bacterial Challenge

Development and antimicrobial susceptibility studies of in vitro monomicrobial and polymicrobial biofilm models with Aspergillus fumigatus and Pseudomonas aeruginosa

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