Traumatized muscle-derived multipotent progenitor cells recruit endothelial cells through vascular endothelial growth factor-A action

Supanc, Heidi R. H.; Gorman, Shannon; Tuan, Rocky S.

doi:10.1002/term.2205

Cited by 2 publications

(1 citation statement)

References 60 publications

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“…Notably, intercellular communication supports simultaneous osteogenesis and angiogenesis in bone tissue engineering in a positive signal feedback loop [9][10][11]. In fact, human mesenchymal stem cells (hMSCs) secrete vasculogenic growth factors, such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor, that enhance angiogenesis, whereas endothelial cells (ECs) produce bone morphogenic proteins, which promote osteoblastic differentiation of precursor bone cells [1,[12][13][14][15]. However, co-culture approaches are complex and parameters such as cell types, culture media, microenvironment, seeding methodology, and scaffold architecture and material will have a significant effect on the final outcome.…”

Section: Introductionmentioning

confidence: 99%

Endothelial cells support osteogenesis in an in vitro vascularized bone model developed by 3D bioprinting

et al. 2020

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Bone is a highly vascularized tissue, in which vascularization and mineralization are concurrent processes during skeletal development. Indeed, both components should be included in any reliable and adherent in vitro model platform for the study of bone physiology and pathogenesis of skeletal disorders. To this end, we developed an in vitro vascularized bone model, using a gelatin-nanohydroxyapatite (gel-nHA) three-dimensional (3D) bioprinted scaffold. First, we seeded human mesenchymal stem cells (hMSCs) on the scaffold, which underwent osteogenic differentiation for 2 weeks. Then, we included lentiviral-GFP transfected human umbilical vein endothelial cells (HUVECs) within the 3D bioprinted scaffold macropores to form a capillary-like network during 2 more weeks of culture. We tested three experimental conditions: condition 1, bone constructs with HUVECs cultured in 1:1 osteogenic medium (OM): endothelial medium (EM); condition 2, bone constructs without HUVECs cultured in 1:1 OM:EM; condition 3: bone construct with HUVECs cultured in 1:1 growth medium:EM. All samples resulted in engineered bone matrix. In conditions 1 and 3, HUVECs formed tubular structures within the bone constructs, with the assembly of a complex capillary-like network visible by fluorescence microscopy in the live tissue and histology. CD31 immunostaining confirmed significant vascular lumen formation. Quantitative real-time PCR was used to quantify osteogenic differentiation and endothelial response. Alkaline phosphatase and runt-related transcription factor 2 upregulation confirmed early osteogenic commitment of hMSCs. Even when OM was removed under condition 3, we observed clear osteogenesis, which was notably accompanied by upregulation of osteopontin, vascular endothelial growth factor, and collagen type I. These findings indicate that we have successfully realized a bone model with robust vascularization in just 4 weeks of culture and we highlighted how the inclusion of endothelial cells more realistically supports osteogenesis. The approach reported here resulted in a biologically inspired in vitro model of bone vascularization, simulating de novo morphogenesis of capillary vessels occurring during tissue development.

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Section: Introductionmentioning

confidence: 99%

Endothelial cells support osteogenesis in an in vitro vascularized bone model developed by 3D bioprinting

et al. 2020

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Enhanced healing activity of burn wound infection by a dextran-HA hydrogel enriched with sanguinarine

et al. 2018

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Burn wounds are associated with a series of risks, such as infection and pathologic scar tissue formation, which significantly delay wound healing and lead to complications. In this study, we successfully fabricated a dextran-hyaluronic acid (Dex-HA) hydrogel enriched with sanguinarine (SA) incorporated into gelatin microspheres (GMs), which had high porosity, good swelling ratio, enhanced NIH-3T3 fibroblast cell proliferation, and sustained SA release profile. The in vitro degradation results indicate that the SA/GMs/Dex-HA hydrogel can be degraded. The in vitro antibacterial tests showed that the SA/GMs/Dex-HA hydrogel can inhibit methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). We evaluated the wound-healing effects and antibacterial properties of SA/GMs/Dex-HA hydrogels in a rat full-thickness burn infection model. The hematoxylin-eosin (H&E) and Masson's trichrome staining results of the SA/GMs/Dex-HA hydrogel showed that it improved re-epithelialization and enhanced extracellular matrix remodeling, and immunohistochemistry results showed that the expression of TGF-β1 and TNF-α was decreased, while the TGF-β3 expression was increased. Our findings suggest that the SA/GMs/Dex-HA hydrogel provides a potential way for infected burn treatment with high-quality and efficient scar inhibition.

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Traumatized muscle-derived multipotent progenitor cells recruit endothelial cells through vascular endothelial growth factor-A action

Cited by 2 publications

References 60 publications

Endothelial cells support osteogenesis in an in vitro vascularized bone model developed by 3D bioprinting

Endothelial cells support osteogenesis in an in vitro vascularized bone model developed by 3D bioprinting

Enhanced healing activity of burn wound infection by a dextran-HA hydrogel enriched with sanguinarine

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