Traveling-Wave-Based Electrodynamic Switch for Concurrent Dual-Polarity Ion Manipulations in Structures for Lossless Ion Manipulations

Attah, Isaac K.; Nagy, Gabe; Garimella, Sandilya; Norheim, Randolph V.; Anderson, Gordon A.; Ibrahim, Yehia; Smith, Richard

doi:10.1021/acs.analchem.9b03987

Cited by 9 publications

(4 citation statements)

References 57 publications

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“…These ions were collected, stored, and released from the same trap at 2.8 s (Figure S5B). Although it has not yet been demonstrated in a SLIM device, the array of ion traps could be implemented using a dual polarity SLIM configuration 41 to enable ion−ion reactions, such as charge reduction or dissociation. 42 Ions collected in different traps do not need to be released in the order they were collected.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Development of a Structure for Lossless Ion Manipulations (SLIM) High Charge Capacity Array of Traps

et al. 2023

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Enhancing the sensitivity of low-abundance ions in a complex mixture without sacrificing measurement throughput is highly desirable. This work demonstrates a way to greatly improve the sensitivity of ion mobility (IM)-selected ions by accumulating them in an array of high-capacity ion traps located inside a novel structures for lossless ion manipulations ion mobility spectrometer (SLIM-IMS) module. The array of ion traps used in this work consisted of seven independently controllable traps. Each trap was 386 mm long and possessed a charge capacity of ∼4.5 × 10 8 charges, with a linear range extending to ∼2.5 × 10 8 charges. Each ion trap could be used to extract a peak (or ions over a mobility range) from an ion mobility separation based on arrival time. Ions could be stored without losses for long times (>100 s) and then released all at once or one trap at a time. It was possible to accumulate large ion populations by extracting and storing ions over repeated IM separations. Enrichment of up to seven individual ion distributions could be performed using the seven independently controllable ion traps. Additionally, the ion trapping process effectively compressed ion populations into narrow peaks, which provides a greatly improved basis for subsequent ion manipulations. The array of high charge capacity ion traps provides a flexible addition to SLIM and a powerful tool for IMS-MS applications requiring high sensitivity.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Development of a Structure for Lossless Ion Manipulations (SLIM) High Charge Capacity Array of Traps

et al. 2023

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“…Cyclic IMS (cIMS) and structures for lossless ion manipulations (SLIM) offer the ability to resolve multiple gas‐phase conformations with extended path lengths compared to traditional IMS techniques. cIMS and SLIM platforms utilize printed circuit boards (PCBs), consisting of closely aligned RF and DC electrodes, that apply electric fields to manipulate ion movement (Attah et al, 2019; Tolmachev et al, 2014). TWIMS, operating at low voltages, is utilized in both cIMS and SLIM devices to separate ions based on mobility differences.…”

Section: Ion Mobilitymentioning

confidence: 99%

Metal adduction in mass spectrometric analyses of carbohydrates and glycoconjugates

Gass

Quintero

Hatvany

et al. 2022

Mass Spectrometry Reviews

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Glycans, carbohydrates, and glycoconjugates are involved in many crucial biological processes, such as disease development, immune responses, and cell–cell recognition. Glycans and carbohydrates are known for the large number of isomeric features associated with their structures, making analysis challenging compared with other biomolecules. Mass spectrometry has become the primary method of structural characterization for carbohydrates, glycans, and glycoconjugates. Metal adduction is especially important for the mass spectrometric analysis of carbohydrates and glycans. Metal‐ion adduction to carbohydrates and glycoconjugates affects ion formation and the three‐dimensional, gas‐phase structures. Herein, we discuss how metal‐ion adduction impacts ionization, ion mobility, ion activation and dissociation, and hydrogen/deuterium exchange for carbohydrates and glycoconjugates. We also compare the use of different metals for these various techniques and highlight the value in using metals as charge carriers for these analyses. Finally, we provide recommendations for selecting a metal for analysis of carbohydrate adducts and describe areas for continued research.

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“…When considering strategies to limit the time ions spend in SLIM, it is important to recall that ions often spend significant time within SLIM undergoing on-board accumulation as a means of boosting sensitivity. Though the benefits of this approach are well-documented, ,, those analyzing labile or highly dynamic species may consider alternative approaches such as the use of ion “dumps” or multiplexing techniques. Approaches that can inject ions via the redirection of a continuous ion beam as opposed to a more traditional gating would not only reduce the time ions spend on the board but would also remove some ambiguity regarding the total time an ion spends within SLIM …”

Section: Discussionmentioning

confidence: 99%

Altering Conformational States of Dynamic Ion Populations using Traveling Wave Structures for Lossless Ion Manipulations

Kinlein,

Clowers

2024

Anal. Chem.

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With its capacity to store and translate ions across considerable distances and times, traveling wave structures for lossless ion manipulations (TW-SLIM) provide the foundation to expand the scope of ion mobility spectrometry (IMS) experiments. While promising, the dynamic electric fields and consequential ionneutral collisions used to realize extensive degrees of separation have a considerable impact on the empirical results and the fundamental interpretation of observed arrival time distributions. Using a custom-designed set of TW-SLIM boards (∼9 m) coupled with a time-of-flight mass spectrometer (SLIM-ToF), we detail the capacity to systematically alter the gas-phase distribution of select peptide conformers. In addition to discussing the role chargetransfer may play in TW-SLIM experiments that occur at extended time scales, the ability of the SLIM-ToF to perform tandem IMS was leveraged to confirm that both the compact and elongated conformers of bradykinin 2+ undergo interconversion within the SLIM. Storage experiments in which ions are confined within SLIM using static potential wells suggest that factors aside from TW-induced ion motion contribute to interconversion. Further investigation into this matter suggests that the use of radio frequency (RF) fields to confine ions within SLIM may play a role in ion heating. Aside from interconversion, storage experiments also provide insight into charge transfer behavior over the course of extended periods. The results of the presented experiments suggest that considerations should be taken when analyzing labile species and inform strategies for the TW-SLIM design and method development.

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Traveling-Wave-Based Electrodynamic Switch for Concurrent Dual-Polarity Ion Manipulations in Structures for Lossless Ion Manipulations

Cited by 9 publications

References 57 publications

Development of a Structure for Lossless Ion Manipulations (SLIM) High Charge Capacity Array of Traps

Development of a Structure for Lossless Ion Manipulations (SLIM) High Charge Capacity Array of Traps

Metal adduction in mass spectrometric analyses of carbohydrates and glycoconjugates

Altering Conformational States of Dynamic Ion Populations using Traveling Wave Structures for Lossless Ion Manipulations

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