Treatment and prevention of inflammatory responses and oxidative stress in patients with obstructive sleep apnea hypopnea syndrome using Chinese herbal medicines

Chen, Qin; Lin, Rong; Hong, Xuchu; Ye, Lin; Lin, Qi‐Chang

doi:10.3892/etm.2016.3484

Cited by 11 publications

(9 citation statements)

References 38 publications

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“…Clinical data pointed out that OSAHS, as one kind of common disease which influence the quality of life for the patients [20]. The current study was designed to explore further about the mechanism of OSAHS in order to search better treatment approaches for this disease.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA ROR mitigates cobalt chloride-induced hypoxia injury through regulation of miR-145

Ge¹,

Liu²,

Liu³

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Obstructive sleep apnoea-hypopnoea syndrome (OSAHS) is a condition causing apnea and hypopnea. LncRNA-ROR revealed properties in regulating hypoxia response. Our study explored the roles of ROR in CoCl 2-induced hypoxia injury in HK-2 cells. HK-2 cells were treated with CoCl 2 to induce hypoxia injury. Cell viability, cell apoptosis and apoptotic proteins were detected using CCK-8, flow cytometry and western blot, respectively. The alter expression of ROR and miR-145 was achieved through transfection. Moreover, the expressions of HIF-a and ERK and MAPK related factors were examined using western blot. We found that CoCl 2 decreased cell viability and increased apoptosis as well as increased the expression of ROR. ROR overexpression increased cell viability, decreased cell apoptosis. ROR overexpression upregulated anti-apoptotic proteins Bcl-2 and decreased p53, Bax and cleaved-Caspase-3. ROR overexpression also increased the expression of HIF-a. On the opposite, ROR silence led to the opposite results as relative to ROR overexpression. ROR overexpression decreased expression of miR-145. Co-transfection with ROR overexpression and miR-145 impaired the promoting effects of ROR in CoCl 2 treated cells. ROR increased phosphorylation of ERK while decreased phosphorylation of MAPK. In conclusion, lncRNA ROR alleviated CoCl 2-induced hypoxia injury through regulation of miR-145 as well as modulating ERK and MAPK signalling. HIGHLIGHTS 1. CoCl 2 induces ROR upregulation; 2. Overexpression of ROR reduces CoCl 2-induced HK-2 cell injury; 3. Silence of ROR promotes CoCl 2-induced HK-2 cell injury; 4. Overexpression of ROR decreases miR-145 expression; 5. ROR overexpression modulates ERK and MAPK signalling pathways through regulation of miR-145.

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Section: Discussionmentioning

confidence: 99%

Long non-coding RNA ROR mitigates cobalt chloride-induced hypoxia injury through regulation of miR-145

Ge¹,

Liu²,

Liu³

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…[28] And PHT is defined by an imbalance with an increase in pulmonary vascular resistance and pulmonary artery pressures. [29] This study explored the effect of miR-130a in OSAHS-associated PHT by targeting the GAX gene, our findings validate that miR-130a might limited the expression of GAX and induced OSAHS-associated PHT.…”

Section: Discussionmentioning

confidence: 99%

“…Evidence showed that the main clinical characteristic of OSAHS is defined by the disturbances in sleep structure and nocturnal hypoxemia and hypercapnia, which may lead to excessive sleepiness in the daytime, cardio-cerebrovascular events, and multiple organ injury that may gravely affect the quality of life (QOL). [ 28 ] And PHT is defined by an imbalance with an increase in pulmonary vascular resistance and pulmonary artery pressures. [ 29 ] This study explored the effect of miR-130a in OSAHS-associated PHT by targeting the GAX gene, our findings validate that miR-130a might limited the expression of GAX and induced OSAHS-associated PHT.…”

Section: Discussionmentioning

confidence: 99%

Role of microRNA-130a in the pathogeneses of obstructive sleep apnea hypopnea syndrome-associated pulmonary hypertension by targeting the GAX gene

Wang

Yang

et al. 2017

Medicine

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Objective:The purpose of this study was to elucidate the role of microRNA-130a (miR-130a) in obstructive sleep apnea hypopnea syndrome (OSAHS)-associated pulmonary hypertension (PHT) by targeting the growth arrest-specific homeobox (GAX) gene.Methods:A total of 108 patients with OSAHS-associated PHT were recruited as the OSAHS-associated PHT group and 110 healthy individuals were randomly selected as the normal control group. Human umbilical vein endothelial cells (HUVECs) were selected and divided into the control, miR-130a mimic, mimic negative control (NC), miR-130a inhibitor, and inhibitor-NC groups. The dual luciferase reporter gene assay was used to identify the relationship between miR-130a and the GAX gene. The quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were applied for the relative expressions of miR-130a and the mRNA and protein expressions of GAX. Serum levels of endothelin-1 (ET-1), vascular endothelial growth factor (VEGF), nitric oxide (NO), and super oxide dismutase (SOD) were detected. Cell apoptosis and angiogenic activity were analyzed by flow cytometry and cell tube formation assay.Results:GAX was a target gene of miR-130a. Compared with the normal control group, the relative expression of miR-130a and the serum levels of ET-1 and VEGF were increased, whereas the mRNA expression of GAX and the serum levels of NO and SOD were decreased in the OSAHS-associated PHT group. Compared with the control, mimic-NC, and inhibitor-NC groups, the relative expressions of miR-130a in the miR-130a mimic group were enhanced, whereas the expression of miR-130a in the miR-130a inhibitor group was reduced. However, the mRNA and protein expressions of GAX showed an opposite trend in the miR-130a mimic and miR-130a inhibitor groups. In comparison to the control, mimic-NC, and inhibitor-NC groups, the miR-130a mimic group had an increase of ET-1 and VEGF expressions, whereas the expressions of NO and SOD were reduced. However, the miR-130a inhibitor group exhibited an opposite trend. The apoptosis rate and tube formation number in the miR-130a mimic group were obviously increased, whereas the miR-130a inhibitor group showed an obvious decrease.Conclusion:These data provided strong evidence that miR-130a may be involved in the progression of OSAHS-associated PHT by down-regulating GAX gene.

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“…Traditional herbal medicine has continued to be widely used for the treatment of oxidative stress [9,10]. Many previous studies have shown that the activity of herbal medicine can relieve oxidative stress through exerting their antioxidation potentials.…”

Section: Introductionmentioning

confidence: 99%

[Retracted] Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide

Zhou

Tang³

et al. 2020

BioMed Research International

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Lonicera japonica is a traditional Chinese herbal medicine with antioxidation, anti-inflammatory, antibacterial, and immunoregulation functions. A method to isolate polysaccharides from Lonicera japonica (LJP) has been reported previously by our group. We also reported previously that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides. In this study, we investigated the protective effect of LJP on cardiomyocytes of mice injured by hydrogen peroxide (H2O2). The results showed that LJP can increase the cardiomyocyte viability and the activities of the enzyme (SOD, CAT, GSH-Px, AST, CPK, and LDH) in cardiomyocytes of mice injured by hydrogen peroxide. The results of intracellular ROS contents showed that a high dose (40 μg mL-1) of LJP had the best effects on protecting the cardiomyocytes of mice injured by H2O2. In addition, the measurement results of the cardiomyocyte apoptosis and the activity of caspase-3, caspase-8, and caspase-9 in cardiomyocytes confirmed this conclusion from another perspective.

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Treatment and prevention of inflammatory responses and oxidative stress in patients with obstructive sleep apnea hypopnea syndrome using Chinese herbal medicines

Cited by 11 publications

References 38 publications

Long non-coding RNA ROR mitigates cobalt chloride-induced hypoxia injury through regulation of miR-145

Long non-coding RNA ROR mitigates cobalt chloride-induced hypoxia injury through regulation of miR-145

Role of microRNA-130a in the pathogeneses of obstructive sleep apnea hypopnea syndrome-associated pulmonary hypertension by targeting the GAX gene

[Retracted] Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide

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