2015
DOI: 10.1016/j.ygyno.2015.03.002
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Treatment monitoring of patients with epithelial ovarian cancer using invasive circulating tumor cells (iCTCs)

Abstract: Goals Contemporary management of epithelial ovarian cancer (EOC) uses biomarkers to monitor response to therapy. This study evaluates the role of invasive circulating tumor cells (iCTCs) in monitoring EOC treatment in comparison with serum cancer antigen 125 (CA125). Methods Molecular and microscopic analyses were used to identify seprase and CD44 as tumor progenitor (TP) markers. The iCTC flow cytometry assay was optimized using blood donated by 64 healthy donors, 49 patients with benign abdominal diseases … Show more

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Cited by 60 publications
(71 citation statements)
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“…The same group also investigated the predictive value of CTC levels in a small group of 31 ovarian cancer patients that received standard taxol/carboplatin chemotherapy, where blood specimens were obtained at different time points, before and after surgery and up to 24 months after chemotherapy treatment. Using the same assay [27], they showed a statistically significant association between CTC levels and disease progression [26].…”
Section: Circulating Tumor Cells (Ctcs)mentioning
confidence: 95%
“…The same group also investigated the predictive value of CTC levels in a small group of 31 ovarian cancer patients that received standard taxol/carboplatin chemotherapy, where blood specimens were obtained at different time points, before and after surgery and up to 24 months after chemotherapy treatment. Using the same assay [27], they showed a statistically significant association between CTC levels and disease progression [26].…”
Section: Circulating Tumor Cells (Ctcs)mentioning
confidence: 95%
“…Blood collection and transport were previously described. 14 Briefly, 2-20 mL of blood was collected from patients using Vacutainer® tubes (Becton Dickinson; green top, sodium heparin as anticoagulant) and processed within 48 hours from collection. Blood was kept at 2-8°C when storage longer than 4 hours was needed.…”
Section: Patients Blood Ascites and Tissue Collection And Tumor mentioning
confidence: 99%
“…To address the low number and heterogeneity of CTCs under investigation, here we used the functional collagen‐based adhesion matrix (CAM) enrichment method capable of enriching both viable bulk tumor cells and TP cells in various cellular sources from patients with high sensitivity to develop a novel patient‐derived CTC model for predicting metastatic progression and treatment response. We identified TP cells by their invasive phenotype including expression of functional CAM uptake and markers for epithelial stem cells such as CD44 and invasiveness (seprase), the method identical to that identifying iCTCs in blood of patients with both metastatic and early stage cancers of the breast, ovary, gastrointestines, lung, pancreas, and prostate . We demonstrated potential roles of patient‐derived iCTCs in metastatic progression and therapeutic response in experimental mice using appearance of mouse iCTCs and micrometastases in the lung or liver as well as after inoculating experimental mice with cells with altered gene expression via RNAi, peptides, and monoclonal antibodies respectively, against seprase.…”
Section: Introductionmentioning
confidence: 99%
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“…The first assay was described approximately 10 years ago and is based on the ability of tumor cells to invade a collagen matrix in vitro. This assay has been used for enumeration and molecular characterization of CTCs in ovarian and prostate cancer (16,17 ), but to our knowledge, its efficiency to culture CTCs remains to be demonstrated.…”
Section: Enrichment Of Live Ctcsmentioning
confidence: 99%