Treatment of Acute Hepatic Failure in Mice by Transplantation of Mixed Microencapsulation of Rat Hepatocytes and Transgenic Human Fetal Liver Stromal Cells

Teng, Yue; Wang, Yunfang; Li, Shuangyan; Wang, Wei; Gu, Ruolan; Guo, Xin; Nan, Xue; Ma, Xiaojun; Pei, Xuetao

doi:10.1089/ten.tec.2009.0374

Cited by 21 publications

(26 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In animal models, serum albumin decreased after acute liver failure induction, but stably returned to normal level when microencapsulated hepatocytes are transplanted [50]. This therapeutic effect, associated to a better survival rate, can be enhanced by transplantation of co-microencapsulated hepatocytes and islets of Langerhans, human umbilical vein endothelial cells or stromal cells [51], [52].…”

Section: Discussionmentioning

confidence: 96%

Impact of Alginate Composition: From Bead Mechanical Properties to Encapsulated HepG2/C3A Cell Activities for In Vivo Implantation

et al. 2013

View full text Add to dashboard Cite

Recently, interest has focused on hepatocytes’ implantation to provide end stage liver failure patients with a temporary support until spontaneous recovery or a suitable donor becomes available. To avoid cell damage and use of an immunosuppressive treatment, hepatic cells could be implanted after encapsulation in a porous biomaterial of bead or capsule shape. The aim of this study was to compare the production and the physical properties of the beads, together with some hepatic cell functions, resulting from the use of different material combinations for cell microencapsulation: alginate alone or combined with type I collagen with or without poly-L-lysine and alginate coatings. Collagen and poly-L-lysine increased the bead mechanical resistance but lowered the mass transfer kinetics of vitamin B12. Proliferation of encapsulated HepG2/C3A cells was shown to be improved in alginate-collagen beads. Finally, when the beads were subcutaneously implanted in mice, the inflammatory response was reduced in the case of alginate mixed with collagen. This in vitro and in vivo study clearly outlines, based on a systematic comparison, the necessity of compromising between material physical properties (mechanical stability and porosity) and cell behavior (viability, proliferation, functionalities) to define optima hepatic cell microencapsulation conditions before implantation.

show abstract

Section: Discussionmentioning

confidence: 96%

Impact of Alginate Composition: From Bead Mechanical Properties to Encapsulated HepG2/C3A Cell Activities for In Vivo Implantation

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Microencapsulation of cells as a useful technique for the delivery of bioactive molecules in vivo has been applied successfully in allogeneic or xenogeneic cell implants for several diseases, such as diabetes, liver failure and hypoparathyroidism [17,20,23]. The main advantage of microcapsules is to protect cells from a host’s immune system [24,25,26].…”

Section: Resultsmentioning

confidence: 99%

“…Microencapsulation using alginate hydrogel has been widely used to overcome immune rejection and prevent the need for immunosuppressive therapy [17,18,19]. Although there is a physical separation of the transplanted cells from the host, small molecules such as erythropoietin and fibroblast growth factor can easily pass through the alginate gel barrier [20,21]. In a previous study, we have shown that met-enkephalin and norepinephrine in the CSF significantly increased following intrathecal implantation of microencapsulated PC12 cells [11].…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain

et al. 2014

IJMS

View full text Add to dashboard Cite

Human pheochromocytoma cells, which are demonstrated to contain and release met-enkephalin and norepinephrine, may be a promising resource for cell therapy in cancer-induced intractable pain. Intrathecal injection of alginate-poly (l) lysine-alginate (APA) microencapsulated human pheochromocytoma cells leads to antinociceptive effect in a rat model of bone cancer pain, and this effect was blocked by opioid antagonist naloxone and alpha 2-adrenergic antagonist rauwolscine. Neurochemical changes of cerebrospinal fluid are in accordance with the analgesic responses. Taken together, these data support that human pheochromocytoma cell implant-induced antinociception was mediated by met-enkephalin and norepinephrine secreted from the cell implants and acting at spinal receptors. Spinal implantation of microencapsulated human pheochromocytoma cells may provide an alternative approach for the therapy of chronic intractable pain.

show abstract

“…Therapies relying on microencapsulated cells could therefore result in the reduction or even avoidance of the administration of immunosuppressive drugs on the one hand and, on the other hand, permit the transplantation of non-human cells, which is a promising alternative considering the limited availability of donor organs [6,7]. The therapeutic potential of the transplantation of microencapsulated cells has been reported for the treatment of a variety of diseases, including liver failure [8][9][10], renal failure [11][12][13], cancer [14], and diabetes mellitus [15][16][17][18].…”

Section: Cell Microencapsulationmentioning

confidence: 99%

Contribution of polymeric materials to progress in xenotransplantation of microencapsulated cells: a review

Mahou

Passemard

Carvello

et al. 2016

Xenotransplantation

View full text Add to dashboard Cite

Cell microencapsulation and subsequent transplantation of the microencapsulated cells require multidisciplinary approaches. Physical, chemical, biological, engineering, and medical expertise has to be combined. Several natural and synthetic polymeric materials and different technologies have been reported for the preparation of hydrogels, which are suitable to protect cells by microencapsulation. However, owing to the frequent lack of adequate characterization of the hydrogels and their components as well as incomplete description of the technology, many results of in vitro and in vivo studies appear contradictory or cannot reliably be reproduced. This review addresses the state of the art in cell microencapsulation with special focus on microencapsulated cells intended for xenotransplantation cell therapies. The choice of materials, the design and fabrication of the microspheres, as well as the conditions to be met during the cell microencapsulation process, are summarized and discussed prior to presenting research results of in vitro and in vivo studies. Overall, this review will serve to sensitize medically educated specialists for materials and technological aspects of cell microencapsulation.

show abstract

Treatment of Acute Hepatic Failure in Mice by Transplantation of Mixed Microencapsulation of Rat Hepatocytes and Transgenic Human Fetal Liver Stromal Cells

Cited by 21 publications

References 26 publications

Impact of Alginate Composition: From Bead Mechanical Properties to Encapsulated HepG2/C3A Cell Activities for In Vivo Implantation

Impact of Alginate Composition: From Bead Mechanical Properties to Encapsulated HepG2/C3A Cell Activities for In Vivo Implantation

Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain

Contribution of polymeric materials to progress in xenotransplantation of microencapsulated cells: a review

Contact Info

Product

Resources

About