Treatment of acute otitis media with an antiadhesive oligosaccharide: a randomised, double-blind, placebo-controlled trial

Ukkonen, Pentti; Varis, Kaapo; Jernfors, Mia; Herva, Elja; Jokinen, Jukka; Ruokokoski, Esa; Zopf, David; Kilpi, Terhi

doi:10.1016/s0140-6736(00)02843-9

Cited by 53 publications

(31 citation statements)

References 25 publications

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“…The antiadhesive therapy was safe and well tolerated but did not suppress or cure H. pylori colonization. Interestingly, a similar compound, 3′-sialyllacto-N-neotetraose, was the subject of a clinical trial for prophylaxis of acute otitis media and used to investigate its effect on nasopharyngeal carriage of bacteria (S. pneumoniae, Haemophilus influenzae, Moraxella catarrhalis) [155]. The compound was given intranasally to 507 children in a randomized, double-blind, placebo-control trial, but did not have a beneficial effect.…”

Section: H Pylori Adhesionmentioning

confidence: 99%

“…The importance of multivalency has been clearly described in the minireview by Pieters [60], which highlights the problems of working with low-affinity interactions between monovalent ligands and may explain the failures of trials such as those with 3′-sialyllactose [154] and 3′-sialyllacto-neotetraose [155]. The dendrimeric scaffolds, described by Pieters [60], offer a future approach to the rational design of high-affinity blockers of bacterial binding.…”

Section: Future Strategies For Carbohydrate-based Antiadhesivesmentioning

confidence: 99%

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Stopping Bacterial Adhesion: A Novel Approach to Treating Infections

Bavington

Page²

2005

Respiration

109

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Adhesion and colonization are prerequisites for the establishment of bacterial pathogenesis. The prevention of adhesion is an attractive target for the development of new therapies in the prevention of infection. Bacteria have developed a multiplicity of adhesion mechanisms commonly targeting surface carbohydrate structures, but our ability to rationally design effective antiadhesives is critically affected by the limitations of our knowledge of the human ‘glycome’ and of the bacterial function in relation to it. The potential for the future development of carbohydrate-based antiadhesives has been demonstrated by a significant number of in vitro and in vivo studies. Such therapies will be particularly relevant for infections of mucosal surfaces where topical application or delivery is possible.

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Section: H Pylori Adhesionmentioning

confidence: 99%

Section: Future Strategies For Carbohydrate-based Antiadhesivesmentioning

confidence: 99%

Stopping Bacterial Adhesion: A Novel Approach to Treating Infections

Bavington

Page²

2005

Respiration

109

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“…Pneumococcus (Streptococcus pneumoniae), Haemophilus influenzae, and Moraxella catarrhalis are part of normal nasopharyngeal (NP) microbial flora and are important causes of acute otitis media in children (4,7). Pneumococcus and H. influenzae serotype b are also leading causes of serious infections, such as pneumonia, meningitis, and bacteremia in children.…”

mentioning

confidence: 99%

“…One milliliter of STGG medium was distributed in screw-cap 3.6-ml tubes, autoclaved at 121°C, and kept at 4°C. For viability subcultures, we used sheep blood agar (BA) without and with gentamicin (5 g/ml) (GBA) plates for S. pneumoniae, enriched chocolate agar (CA) plates for H. influenzae, and BA plates for M. catarrhalis (6,7).…”

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confidence: 99%

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Survival of Streptococcus pneumoniae , Haemophilus influenzae , and Moraxella catarrhalis Frozen in Skim Milk- Tryptone-Glucose-Glycerol Medium

Kaijalainen

Ruokokoski²,

Ukkonen³

et al. 2004

J Clin Microbiol

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In STGG (skim milk, tryptone, glucose, glycerol) medium at ؊80°C, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates survived for at least 3 years, and the same species have survived in nasopharyngeal swabs for at least 1.5 years. At ؊20°C, S. pneumoniae and M. catarrhalis survived for 1.5 years, but H. influenzae survived for only 2 months.Pneumococcus (Streptococcus pneumoniae), Haemophilus influenzae, and Moraxella catarrhalis are part of normal nasopharyngeal (NP) microbial flora and are important causes of acute otitis media in children (4, 7). Pneumococcus and H. influenzae serotype b are also leading causes of serious infections, such as pneumonia, meningitis, and bacteremia in children. Vaccines against pneumococcus and H. influenzae serotype b are serotype specific, and the prevalence of the different types in different geographic areas should be known. Reliable methods are needed for the collection, transport, and storage of both NP samples and bacterial isolates from study sites to specialized laboratories even in developing country settings. STGG (skim milk, tryptone, glucose, glycerol) medium (1, 2) has been used successfully for storage at Ϫ70 to Ϫ80°C of isolates of S. pneumoniae and H. influenzae (2) and of NP swabs for detection of the same bacteria (3, 4).In this study, we used STGG medium (1, 2) as described by O'Brien and coworkers (4). One milliliter of STGG medium was distributed in screw-cap 3.6-ml tubes, autoclaved at 121°C, and kept at 4°C. For viability subcultures, we used sheep blood agar (BA) without and with gentamicin (5 g/ml) (GBA) plates for S. pneumoniae, enriched chocolate agar (CA) plates for H. influenzae, and BA plates for M. catarrhalis (6, 7).We first tested survival of S. pneumoniae (ATCC 6305), M. catarrhalis (ATCC 25238), and H. influenzae (a clinical isolate) isolates in 1 ml of STGG in 3.6-ml screw-cap tubes. For S. pneumoniae, three STGG tubes were prepared for each temperature (Ϫ20°C and Ϫ80°C) by inoculation with (i) five colonies lightly touched with a 1-l loop, (ii) a 1-l loopful, or (iii) plenty of growth. For H. influenzae and for M. catarrhalis, one tube for each temperature was inoculated with plenty of growth. One further tube for each temperature was inoculated with plenty of all the three bacteria. The tubes were kept at Ϫ20 and Ϫ80°C, and subcultures for viability testing were performed 18 times over a period of 3 years at variable time intervals (see Fig. 1 and Results).For subculturing, the STGG tubes were taken from the freezers and kept on the bench (at room temperature) until thawed, the contents were mixed by vortexing, and 10 l of each bacterial suspension was streaked on the appropriate plate. The tubes were transferred immediately into freezers. The plates were incubated at 37°C for 18 to 24 h. Bacterial colonies were counted, and the results of this process were expressed as follows: no growth (Ͻ1 colony), 1 to 20 colonies, 21 to 100 colonies, and Ͼ100 colonies.The S. pneumoniae and M. catarrhalis isolates survi...

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Bioactivity of Human Milk Oligosaccharides

Kunz

Rudloff

2014

Food Oligosaccharides

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Treatment of acute otitis media with an antiadhesive oligosaccharide: a randomised, double-blind, placebo-controlled trial

Cited by 53 publications

References 25 publications

Stopping Bacterial Adhesion: A Novel Approach to Treating Infections

Stopping Bacterial Adhesion: A Novel Approach to Treating Infections

Survival of Streptococcus pneumoniae , Haemophilus influenzae , and Moraxella catarrhalis Frozen in Skim Milk- Tryptone-Glucose-Glycerol Medium

Bioactivity of Human Milk Oligosaccharides

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