Treatment of Acute Wounds With Recombinant Human-Like Collagen and Recombinant Human-Like Fibronectin in C57BL/6 Mice Individually or in Combination

Abstract

Wound repair is accomplished by the interaction between the cells involved in the repair and the extracellular matrix (ECM). Collagen is the main component of ECM, which is involved in transduction of signal, transportation of growth factors and cytokines. Fibronectin (FN) is also an important ECM, which participates in the initiation of fibroblast cell (FC) and promotes adhesion, migration, proliferation and differentiation of target cells. Compared with natural protein, the recombinant protein prepared by artificial method has the advantages of poor immunogenicity, wide range of sources, low cost and high activity. In this study, we used recombinant human-like collagen (RHC) and recombinant human-like fibronectin (rhFN) to treat acute wounds in C57BL/6 mice individually or in combination, and explored their effects on wound healing. Our study confirmed that these two recombinant proteins could effectively promote the proliferation, migration and adhesion of FCs. Meanwhile, it could positively regulate the healing speed and quality of acute wounds, re-epithelialization, collagen deposition, inflammation and angiogenesis. Moreover, we proved that the combination of the two was better than the treatment alone. Consequently, it has a good prospect as a new tissue material in the field of skin repair.

Combination of fractional carbon dioxide laser with recombinant human collagen in periocular skin rejuvenation

“…This can offer more consistent and biochemically identical human collagen in a factory mode 5 . The rhCOLI and rhCOLIII promote human dermal fibroblasts infiltration, neovascularization, stimulating collagen synthesis and elastin production, and modulate the inflammatory response by downregulating pro‐inflammatory cytokines (TNF‐α, IL‐1β, and IL‐6) and upregulating anti‐inflammatory cytokines (TGF‐β1), thus allowing it to withstand or resist the damaging effects of inflammation, and leading to ECM assembly and wound healing 16–20 . However, the large molecular size of the recombinant collagens limits their topical application because of the innate barrier property of the skin.…”

“…5 The rhCOLI and rhCOLIII promote human dermal fibroblasts infiltration, neovascularization, stimulating collagen synthesis and elastin production, and modulate the inflammatory healing. [16][17][18][19][20] However, the large molecular size of the recombinant collagens limits their topical application because of the innate Note: Income per month: 1: less than ¥5, 000; 2: ¥5000-10 000; 3: ¥10 000-20 000; 4: more than ¥20 000.…”

Recombinantly expressed rhFEB remodeled the skin defect of db/db mice

“…The dynamic imbalance between the synthesis and decomposition of collagen is an important factor leading to the slow healing of diabetic wounds in the inflammatory stage (Dinh et al 2012 ). In the later stages of wound healing, collagen facilitates the migration, re-epithelization, and deposition of keratinocytes (Chen et al 2019 ; Dong et al 2022 ) as well as the synthesis of FN in the wound tissue (Lai et al 2020 ). Wound healing is accompanied by the conversion of COL3A1 to COL1A1, which is the result of FN recognizing and binding to COL3A1 during the proliferative phase (Beyeler et al 2019 ).…”