Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency

Uno, Narumi; Satofuka, Hiroyuki; Miyamoto, Hitomaru; Honma, Kazuhisa; Suzuki, Tomohiko; Yamazaki, Kyotaro; Ito, Ryo; Moriwaki, Takashi; Hamamichi, Shusei; Tomizuka, Kazuma; Oshimura, Mitsuo; Kazuki, Yasuhiro

doi:10.1016/j.omtn.2023.07.002

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…Taxol is a microtubule stabilizer while reversine is an inhibitor of the spindle assembly checkpoint. The combination of 400 nM Taxol and 500-1500 nM reversine was shown to be superior to colcemid and also to the combination of two mitotic spindle disruptors (TN-16 and griseofulvin) in several cell lines and with different fusogens [23]. In addition, Liskovykh and coworkers showed that the substitution of Cytochalasin B with Latrunculin B significantly increased (p < 0.0001) the efficiency of MMCT [24].…”

Section: Step 1: Donor Cell Line and Micronucleationmentioning

confidence: 98%

“…cell lines and with different fusogens [23]. In addition, Liskovykh and coworkers showed that the substitution of Cytochalasin B with Latrunculin B significantly increased (p < 0.0001) the efficiency of MMCT [24].…”

Section: Step 2: Fusion and Selectionmentioning

confidence: 99%

“…The classical protocol makes use of colcemid, a microtubule destabilizer, followed by disarrangement of the actin cytoskeleton using Cytochalasin B, to promote microcell formation. Recently, Uno and coworkers have shown that both microcell production and chromosome transfer efficiency can be improved by substituting colcemid with an association of taxol (paclitaxel) and reversine [23]. Taxol is a microtubule stabilizer while reversine is an inhibitor of the spindle assembly checkpoint.…”

Section: Step 1: Donor Cell Line and Micronucleationmentioning

confidence: 99%

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Chromosome Transplantation: Opportunities and Limitations

La Grua,

Rao,

Susani

et al. 2024

Cells

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There are thousands of rare genetic diseases that could be treated with classical gene therapy strategies such as the addition of the defective gene via viral or non-viral delivery or by direct gene editing. However, several genetic defects are too complex for these approaches. These “genomic mutations” include aneuploidies, intra and inter chromosomal rearrangements, large deletions, or inversion and copy number variations. Chromosome transplantation (CT) refers to the precise substitution of an endogenous chromosome with an exogenous one. By the addition of an exogenous chromosome and the concomitant elimination of the endogenous one, every genetic defect, irrespective of its nature, could be resolved. In the current review, we analyze the state of the art of this technique and discuss its possible application to human pathology. CT might not be limited to the treatment of human diseases. By working on sex chromosomes, we showed that female cells can be obtained from male cells, since chromosome-transplanted cells can lose either sex chromosome, giving rise to 46,XY or 46,XX diploid cells, a modification that could be exploited to obtain female gametes from male cells. Moreover, CT could be used in veterinary biology, since entire chromosomes containing an advantageous locus could be transferred to animals of zootechnical interest without altering their specific genetic background and the need for long and complex interbreeding. CT could also be useful to rescue extinct species if only male cells were available. Finally, the generation of “synthetic” cells could be achieved by repeated CT into a recipient cell. CT is an additional tool for genetic modification of mammalian cells.

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Section: Step 1: Donor Cell Line and Micronucleationmentioning

confidence: 98%

Section: Step 2: Fusion and Selectionmentioning

confidence: 99%

Section: Step 1: Donor Cell Line and Micronucleationmentioning

confidence: 99%

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Chromosome Transplantation: Opportunities and Limitations

La Grua,

Rao,

Susani

et al. 2024

Cells

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Microcell-mediated chromosome transfer between non-identical human iPSCs

Uno,

Miyamoto,

Yamazaki

et al. 2024

Molecular Therapy - Nucleic Acids

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Diving into paclitaxel: isolation and screening content from Taxus sumatrana at Singgalang Conservation Center, West Sumatra

Kurniawan,

Sukrasno,

Ashari

et al. 2024

Natural Product Research

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The report features the first isolation of paclitaxel from wood of conservated T. sumatrana. The T. sumatrana is a nationally protected endemic plant that has been successfully cultivated outside its natural habitat at the Singgalang Conservation Centre in West Sumatra. The paclitaxel was utilized as a reference standard for evaluating its presence in different parts of T.sumatrana. This analysis exhibits that the acetone extract from T. sumatrana bark contained the highest paclitaxel concentration, measuring about 0.473 ± 0.031 ppm. The isolated paclitaxel demonstrated potent cytotoxic activity against A549, HeLa, and MCF7 cancer cells, by IC50 values of 3.26 ± 0.334, 2.85 ± 0.257, and 3.81 ± 0.013 μM, respectively. This outcome provides scientific support for conservation programs and campaigns for the community to engage in conservation efforts.

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Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency

Cited by 6 publications

References 40 publications

Chromosome Transplantation: Opportunities and Limitations

Chromosome Transplantation: Opportunities and Limitations

Microcell-mediated chromosome transfer between non-identical human iPSCs

Diving into paclitaxel: isolation and screening content from Taxus sumatrana at Singgalang Conservation Center, West Sumatra

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