Treatment of controlled pore glass with poly(ethylene oxide) to prevent adsorption of rabies virus

Hiatt, C. W.; Shelokov, Alexis; Rosenthal, Edward J.; Galimore, James M.

doi:10.1016/s0021-9673(00)97825-7

Cited by 78 publications

(9 citation statements)

References 5 publications

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“…However, viruses have been shown to elute in wide bands from these columns (Hiatt et al 1971), supporting our findings that CPG does not have the resolution necessary to separate complex assemblages of aquatic viruses.…”

Section: Column Chromatographysupporting

confidence: 86%

“…In previous studies, controlled-pore glass has been used to separate viruses from debris in lysates by eluting the viruses in the exclusion volume (Gschwender et al 1969), and two viruses differing widely in size and shape have previously been separated in this way (Haller 1965). However, viruses have been shown to elute in wide bands from these columns (Hiatt et al 1971), supporting our findings that CPG does not have the resolution necessary to separate complex assemblages of aquatic viruses.…”

Section: V) Size-exclusion Chromatography With Sephacrylsupporting

confidence: 79%

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Physical fractionation of aquatic viral assemblages

Brum

Steward

2011

Limnology & Ocean Methods

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Aquatic viruses are extraordinarily diverse and have major influences on plankton ecology and evolution, but the majority of these viruses remain uncharacterized. Most viruses have not been cultivated and cultivationindependent means to explore viral diversity have provided only a fragmented view of viral genomic information. In this study, ultracentrifugation and chromatographic methods were evaluated for their ability to fractionate aquatic viruses based on their differing physical properties with the aim of physically enriching subsets of aquatic viruses for further study. Centrifugation in continuous cesium chloride gradients, strong and weak anion‐exchange chromatography, and size‐exclusion chromatography with Sephacryl S‐1000 were found to separate aquatic viruses based on their differing buoyant densities, surface charges, and sizes, respectively. Sucrose density gradients, cation‐exchange chromatography, and size‐exclusion chromatography using controlled‐pore glass were found to have insufficient resolution to effectively separate viral assemblages. Using the successful methods listed above, we were able to separate complex assemblages of viruses into fractions that had distinguishable subsets of the viruses present in the original community as determined by pulsed‐field gel electrophoresis. Strong anion‐exchange chromatography was particularly effective at separating viruses in the samples and resulted in one to six dominant viral genome bands in most fractions. Our results suggest that individual populations of viruses may be physically enriched, and possibly isolated, from complex assemblages without cultivation. These fractionation methods are expected to improve our ability to characterize the genotypes and phenotypes of the uncultivated majority of viruses in aquatic environments.

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Section: Column Chromatographysupporting

confidence: 86%

Section: V) Size-exclusion Chromatography With Sephacrylsupporting

confidence: 79%

Physical fractionation of aquatic viral assemblages

Brum

Steward

2011

Limnology & Ocean Methods

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“…Using the surface force technique, Luckam [22,23] observed that steric repulsion with PEO is mainly due to osmotic repulsion between interdigitated PEO chains. Jeon et al [24,25] Interfacial properties of PEO-treated biomaterials PEO-rich surfaces have been prepared by physical adsorption of PEO [26,27]. Only high molecular weight PEO (Mw > 100000) can be effectively adsorbed on hydrophobic surfaces [28].…”

Section: Steric Repulsion With Terminally Attached Peomentioning

confidence: 99%

Surface modification of polymeric biomaterials with poly(ethylene oxide), albumin, and heparin for reduced thrombogenicity

Amiji

Park

1993

Journal of Biomaterials Science, Polymer Edition

327

142

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Appropriate surface modification has significantly improved the blood compatibility of polymeric biomaterials. This article reviews methods of surface modification with water-soluble polymers, such as polyethylene oxide (PEO), albumin, and heparin. PEO is a synthetic, neutral, water-soluble polymer, while albumin and heparin are a natural globular protein and an anionic polysaccharide, respectively. When grafted onto the surface, all three macromolecules share a common feature to reduce thrombogenicity of biomaterials. The reduced thrombogenicity is due to the unique hydrodynamic properties of the grafted macromolecules. In aqueous medium, surface-bound water-soluble polymers are expected to be highly flexible and extend into the bulk solution. Biomaterials grafted with either PEO, albumin, or heparin are able to resist plasma protein adsorption and platelet adhesion predominantly by a steric repulsion mechanism.

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“…PEO surfaces have but for other areas as well, such as membranes for been prepared by physical adsorption of PEO onto exseparation processes, biosensors, chromatographic isting substrates. [18][19][20][21] Only high-molecular-weight PEO supports, contact lenses, immunoassay, blood, and (mol. wt.…”

Section: Introductionmentioning

confidence: 99%

Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces

Lee¹,

Jeong

Lee

1997

J. Biomed. Mater. Res.

146

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Comb-like polyethylene oxide (PEO) surfaces were prepared on low-density polyethylene (PE). The comb-like PEO chain density was changed gradually along the sample lengths by corona discharge treatment with gradually increasing power and the following graft copolymerization of poly(ethylene glycol) monomethacrylate macromers (PEO-MA). The macromers with different PEO repeat unit, 1, 5, and 10, were used. The prepared comb-like PEO gradient surfaces were characterized by water contact angle, Fourier transform infrared spectroscopy in the attenuated total reflectance mode, and electron spectroscopy for chemical analysis. All these measurements indicated that the PEO chains are grafted on the PE surface with gradually increasing density of PEO. Plasma protein adsorption and platelet adhesion on the PEO gradient surfaces decreased with increasing PEO chain length and surface density. As observed by scanning electron microscopy, PEO10-MA-grafted surface with high PEO density was very effective in preventing protein adsorption and platelet adhesion and did not activate the platelets.

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Treatment of controlled pore glass with poly(ethylene oxide) to prevent adsorption of rabies virus

Cited by 78 publications

References 5 publications

Physical fractionation of aquatic viral assemblages

Physical fractionation of aquatic viral assemblages

Surface modification of polymeric biomaterials with poly(ethylene oxide), albumin, and heparin for reduced thrombogenicity

Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces

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