This study evaluates the efficacy of e-beam irradiation in anchovy drying from an initial moisture content of 78 ± 1% wb to 20 ± 1% wb. The anchovy was irradiated with a 10 MeV energy level and doses ranging from 3 to 7 kGy, with air temperatures of around 30°C. Microbial quality was assessed at the Intertek laboratory before and after irradiation. Results showed that parameters such as Clostridium perfringens, Coliforms, E. coli, Salmonella spp., Staphylococcus aureus, Vibrio parahaemolyticus Bacteria, Yeasts and Moulds, and TPC (30°C) met the expected results at a dose of 7 kGy, demonstrating the quality of anchovy products for export. This study highlights the potential of e-beam irradiation in the inactivation of pathogenic microorganisms without compromising food quality in anchovy products.