Measurements are reported for the radiation pressure and gas drag forces acting on a single melamine-formaldehyde microsphere. The radiation pressure force coefficient q, which would be unity if all incident photons were absorbed, has the value qϭ0.94Ϯ0.11. For argon, the Epstein gas drag force coefficient ␦, which would be unity if impinging molecules underwent specular reflection, has the value ␦ϭ1.26Ϯ0.13 as measured with our single-particle laser acceleration method, or ␦ϭ1.44Ϯ0.19 as measured using the vertical resonance method.