Treatment of hepatocellular carcinoma: Steps forward but still a long way to go

Mlynarsky, Liat; Menachem, Yoram; Shibolet, Oren

doi:10.4254/wjh.v7.i3.566

Cited by 45 publications

(31 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…And the result of qRT-PCR showed that the osteogenic differentiation marker genes, including Runx2, OCN, and BMP-2, were significantly increased in pCMV-sh-DANCR group. We performed ALP activity and alizarin red staining [15] assays to measure cell osteogenic differentiation, and the results show that both ALP and alizarin red staining levels were higher in pCMV-sh-DANCR group. All these results suggested that downregulation of lncRNA DANCR promotes osteogenic differentiation of PDLSCs.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells

Wang

Huang

Tan

2019

Preprint

View full text Add to dashboard Cite

Backgrounds: Long non-coding RNAs (lncRNAs) have been widely known to have an appreciable effect in physiology and pathology. In tooth regeneration, periodontal ligament stem cells (PDLSCs) are regarded as a key effector, whereas, how lncRNA acts in the osteogenic differentiation of PDLSCs have not been completely understood. This study aims to find out the relationship between lncRNA DANCR and the proliferation and osteogenic differentiation of PDLSCs. Method: Microarray was used to observe the different expression of lncRNAs in differentiated and undifferentiated PDLSCs. And then osteogenic-related lncRNA, DNACR was screened out. To explore its effects on proliferation and osteogenic differentiation by constructing an overexpression and inhibition model. qRT-PCR was used to detect the mRNA expression of osteogenesis related genes. MTT assay was performed to assess the effects of DNACR on cell growth curve. To quantify the effects of DNACR on osteogenic differentiation of PDLCs, ALP staining and alizarin red was performed in basic culture medium and osteogenic medium. Data were statistically processed. Results: Compared with the undifferentiated PDLSCs, the alizarin red staining level was higher in differentiated PDLSCs. And the expressions of osteogenic differentiation marker genes Runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and bone morphogenetic protein (BMP-2) were significantly increased in the differentiated PDLSCs. Furthermore, we noticed that comparing with control groups, the expression of LncRNA DANCR decreases markedly in osteogenically induced PDLSCs. DANCR promoted proliferation of PDLSCs, as evidenced by cell viability. Further investigation has proven that the downregulation of DANCR shows in the calcium sediment forming, alkaline phosphatase (ALP) activation and some osteogenic-related gene markers’ upregulation including Runx2, OCN and BMP-2, which finally results in the osteogenic differentiation of PDLSCs following the transfection and induction. Conversely, DANCR upregulation was shown to repress the osteogenic differentiation potential of PDLSCs. Conclusions: The osteogenic differentiation of PDLSCs has proven to related to the down regulation of lncRNA DANCR. And this paper throws light on the effects of DANCR in the process of PDLSCs’ osteogenic differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%

Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells

Wang

Huang

Tan

2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Hepatocellular carcinoma (HCC) is one of the most aggressive malignant tumors with respect to the disease course and mortality rate (1) Although numerous therapeutic strategies have been introduced, the prognosis of patients with HCC remains poor due to the frequent metastasis and recurrence (2,3). Therefore, it is crucial to identify specific molecular targets and provide the basis for early diagnosis of HCC and targeted therapy, in order to achieve optimal outcomes and improve the quality of life and life expectancy of HCC patients.…”

Section: Introductionmentioning

confidence: 99%

miRNA expression profiling regulates necroptotic cell death in hepatocellular carcinoma

et al. 2018

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is one of the most aggressive types of cancer and is among the leading causes of cancer-related mortality worldwide. Although the dysregulation of microRNAs (miRNAs or miRs) has often been reported in HCC, the precise molecular mechanisms by which miRNAs modulate the process of tumorigenesis and the behavior of cancer cells are not yet clearly understood. In this study, we identified a novel three‑miRNA signature, including miR‑371-5p, miR‑373 and miR‑543, that appears to orchestrate programmed cell necrosis in HCC by directly targeting the caspase‑8 gene (Casp‑8). Our results demonstrated that miR‑371-5p, miR‑373 and miR‑543 were overexpressed in HCC tissues compared with paired adjacent normal tissues. The upregulation of these miRNAs specifically and markedly downregulated the expression of Casp‑8, as well as significantly enhanced the Z-VAD/TNF‑α-induced necroptosis of HCC cells. By contrast, the selective knockdown of miRNA expression led to a significant increase in Casp‑8 levels and a marked reduction in programmed cell necrosis. Intriguingly, the sustained overexpression of Casp‑8 reversed the pro‑necroptotic effects exerted by miRNA mimics. Finally, a strong inverse association between the level of miR‑223 and the expression levels of nucleotide-binding oligomerization domain-like receptor family, pyrin domain-containing-3 inflammasome was observed in our HCC specimens. On the whole, the present study revealed a molecular link between the three‑miRNA signature, comprising miR‑371-5p, miR‑373 and miR‑543, and the negative necroptotic regulator Casp‑8, and presents evidence for its employment as a novel potential diagnostic, prognostic and therapeutic target in HCC.

show abstract

“…Due to the lack of effective diagnostic and screening strategies, most of the HCC patients are diagnosed late. Depending on the stage of the cancer, multiple different treatment modalities like transplantation, partial hepatectomy, chemo-embolization, systemic chemotherapy could be given [5,6] . Among these approaches, the only potentially available curative therapy is resection and liver transplantation which can only be applied in the early stages of HCC before metastasis are detected [5] .…”

Section: Introductionmentioning

confidence: 99%

T cell immunotherapy in hepatitis B Virus related hepatocellular carcinoma

Hafezi¹,

Bertoletti²,

Tan³

2018

View full text Add to dashboard Cite

Chronic hepatitis B virus (HBV) infection is a major risk factor for hepatocellular carcinoma (HCC). While multiple treatment modalities are available, liver transplantation remains the sole curative treatment for advanced stages of HCC, and hence new treatment approaches are required to fulfill this unmet need of curative HCC therapy. Our first-in-man proof-of-concept adoptive T-cell immunotherapy against HBV related hepatocellular carcinoma metastases has shown promising results. Here, we review the development of T-cell immunotherapy targeting HBV antigens for the treatment of HBV-HCC and discuss the practical considerations for the safe and effective use in clinics.

show abstract

Treatment of hepatocellular carcinoma: Steps forward but still a long way to go

Cited by 45 publications

References 61 publications

Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells

Downregulation of lncRNA DANCR promotes osteogenic differentiation of periodontal ligament stem cells

miRNA expression profiling regulates necroptotic cell death in hepatocellular carcinoma

T cell immunotherapy in hepatitis B Virus related hepatocellular carcinoma

Contact Info

Product

Resources

About