Treatment of Intervertebral Disc Degeneration in Wistar Rats with Mesenchymal Stem Cells

Лыков, А. П.; Bondarenko, N. A.; Повещенко, О. В.; Kim, I. I.; Суровцева, М. А.; Sadykova, J B; Semin, P A; Zavjalov, Evgenii L.; Кривошапкин, А. Л.; Vi, Konenkov

doi:10.1007/s10517-020-04756-2

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…The recruitment and activation of endogenous stem cells to repair degenerated intervertebral discs avoid the iatrogenic injury of intervertebral discs that cannot be avoided by the transplantation of exogenous cells 20,27 . Wang et al transplanted nucleus pulposus, mesenchymal stem cell, a rabbit model of intervertebral disc degeneration was made by placing algae cells and bone marrow stem cells on alginate scaffolds; the results showed that CESCs had the best regeneration ability, and in vitro differentiation, and they also found that CESCs overmatched bone marrow stem cells, NPSCs and AFSCs in cartilage and osteogenesis; the results showed that CESCs as seed cells showed better application potential in intervertebral disc tissue engineering 11,28 . Transplanted CESCs compound hydroxyapatite subcutaneously into nude mice.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus

Guan

Sun

Zou

et al. 2021

J Cellular Molecular Medi

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To investigate the regulatory effect of carbohydrate sulfotransferase 3 (CHST3) in cartilage endplate‐derived stem cells (CESCs) on the molecular mechanism of intervertebral disc degeneration after nucleus pulposus repair in rats. We performed GO and KEGG analysis of GSE15227 database to select the differential genes CHST3 and CSPG4 in grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration, IHC and WB to detect the protein profile of CHST3 and CSPG4, Co‐IP for the interaction between CHST3 and CSPG4. Then, immunofluorescence was applied to measure the level of CD90 and CD105, and flow cytometry indicated the level of CD73, CD90 and CD105 in CESCs. Next, Alizarin red staining, Alcian blue staining and TEM were performed to evaluate the effects of CESCs into osteoblasts and chondroblasts, respectively, CCK8 for the cell proliferation of osteoblasts and chondroblasts after induction for different times; cell cycle of osteoblasts or chondroblasts was measured by flow cytometry after induction, and WB for the measurement of specific biomarkers of OC and RUNX in osteoblasts and aggrecan, collagen II in chondroblasts. Finally, colony formation was applied to measure the cell proliferation of CESCs transfected with ov‐CHST3 or sh‐CHST3 when cocultured with bone marrow cells, WB for the protein expression of CHST3, CSPG4 and ELAVL1 in CSECs, transwell assay for the migration of CESCs to bone marrow cells, TEM image for the cellular characteristics of bone marrow cells, and WB for the protein profile of VCAN, VASP, NCAN and OFD1 in bone marrow cells. CHST3 and CSPG4 were differentially expressed and interacted in grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration; CD73, CD90 and CD105 were lowly expressed in CESCs, osteogenic or chondroblastic induction changed the characteristics, proliferation, cell cycle and specific biomarkers of osteoblasts and chondroblasts after 14 or 21 days,; CHST3 affected the cell proliferation, protein profile, migration and cellular features of cocultured CESCs or bone marrow cells. CHST3 overexpression promoted CESCs to regulate bone marrow cells through interaction with CSPG4 to repair the grade Ⅱ, Ⅲ and Ⅳ intervertebral disc degeneration.

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Section: Discussionmentioning

confidence: 99%

Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus

Guan

Sun

Zou

et al. 2021

J Cellular Molecular Medi

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“…Терапевтический потенциал МСК показан и при критической ишемии нижних конечностей -улучшение перфузии крови, стимуляция ангиогенеза, уменьшение аутоампутаций, мышечной дистрофии и фиброза [80][81][82][83]. МСК нашли свое применение при лечении заболеваний опорно-двигательной системы [84][85][86][87], способствуют сохранности клеток пульпозного ядра межпозвонкового диска, снижению воспалительной реакции. МСКориентированные технологии применяются для лечения дефектов кожи (длительно незаживающие язвенные дефекты различного генеза, ожоги).…”

Section: терапевтический потенциал мскunclassified

Mesenchymal stem cells: properties and clinical application

Лыков

2023

Sibirskij nauchnyj medicinskij zhurnal

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Mesenchymal stem cells (MSCs) are multipotent stromal cells that can diﬀerentiate into various cell types, including adipocytes, osteocytes, chondrocytes, etc. MSCs can be isolated from various human and animal tissues. MSCs are characterized by high proliferative capacity, diﬀerentiation in the connective-tissue direction, paracrine and trophic activity (they produce a wide range of biologically active molecules), are capable of migrating to the zone of organ and tissue damage, and exhibit immunosuppression. There are similarities and diﬀerences between human and mammalian MSCs in phenotype and functional activity. The therapeutic potential of MSCs has been shown on experimental models and in clinical trials, which allows us to consider the MSC-oriented cell technologies as an alternative to traditional methods of treatment. The article presents a review and analysis of the literature devoted to the study of MSCs properties, signaling pathways involved in the regulation of cell activity, the prospects for the use of MSCs in the treatment of inﬂammatory and degenerative diseases. During preparation of the review full-text, free access articles for the period from 2006 to 2022 were taken from eLibrary and National Center for Biotechnology Information (NCBI) databases.

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“…After a period of time, both imaging and histological findings showed that the IVD height and the composition of NP cells were improved. It was also confirmed that EPO could inhibit MSC migration under oxidative stress and stimulate MSC regeneration through local Table 2 The application of BMSCs in vitro ADMSCs adipose mesenchymal stem cells, AF annulus fibrosus, BMSCs bone marrow mesenchymal stem cells, BMSCs-exos bone marrow mesenchymal stem cells exsomes, COL I collagen type I, COL II collagen type II, ECM extracellular matrix, MAPK mitogen activated protein kinase, MMPs matrix metalloproteinases, MSCs mesenchymal stem cells, NP nucleus pulposus, NP-exos nucleus pulposus exsomes, TGF transforming growth factor, TNF tumor necrosis factor accumulation [53]. Yi et al [54] obtained transgenic rabbit BMSCs (TBTs) by transfection with a recombinant adenovirus vector carrying human metalloproteinase tissue inhibitor (hTIMP)-1, and implanted them into the rabbit IDD model.…”

Section: Bmscsmentioning

confidence: 99%

Application of stem cells in the repair of intervertebral disc degeneration

et al. 2022

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Intervertebral disc degeneration (IDD) is a common disease that increases with age, and its occurrence is stressful both psychologically and financially. Stem cell therapy for IDD is emerging. For this therapy, stem cells from different sources have been proven in vitro, in vivo, and in clinical trials to relieve pain and symptoms, reverse the degeneration cascade, delay the aging process, maintain the spine shape, and retain mechanical function. However, further research is needed to explain how stem cells play these roles and what effects they produce in IDD treatment. This review aims to summarize and objectively analyse the current evidence on stem cell therapy for IDD.

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Treatment of Intervertebral Disc Degeneration in Wistar Rats with Mesenchymal Stem Cells

Cited by 9 publications

References 13 publications

Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus

Carbohydrate sulfotransferase 3 (CHST3) overexpression promotes cartilage endplate‐derived stem cells (CESCs) to regulate molecular mechanisms related to repair of intervertebral disc degeneration by rat nucleus pulposus

Mesenchymal stem cells: properties and clinical application

Application of stem cells in the repair of intervertebral disc degeneration

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